Trends in recombinant protein use in animal production

Abstract

Recombinant technologies have made possible the production of a broad catalogue of proteins of interest, including those used for animal production. The most widely studied proteins for the animal sector are those with an important role in reproduction, feed efficiency, and health. Nowadays, mammalian cells and fungi are the preferred choice for recombinant production of hormones for reproductive purposes and fibrolytic enzymes to enhance animal performance, respectively. However, the development of low-cost products is a priority, particularly in livestock. The study of cell factories such as yeast and bacteria has notably increased in the last decades to make the new developed reproductive hormones and fibrolytic enzymes a real alternative to the marketed ones. Important efforts have also been invested to developing new recombinant strategies for prevention and therapy, including passive immunization and modulation of the immune system. This offers the possibility to reduce the use of antibiotics by controlling physiological processes and improve the efficacy of preventing infections. Thus, nowadays different recombinant fibrolytic enzymes, hormones, and therapeutic molecules with optimized properties have been successfully produced through cost-effective processes using microbial cell factories. However, despite the important achievements for reducing protein production expenses, alternative strategies to further reduce these costs are still required. In this context, it is necessary to make a giant leap towards the use of novel strategies, such as nanotechnology, that combined with recombinant technology would make recombinant molecules affordable for animal industry.

Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

“…Stimulation of ovarian activity was widely explored through nutrition (Downing et al, 1995;Gifre et al, 2017), hormonal administration (Mandiki et al, 1993;Zarazaga et al, 1996), both nutritional and hormonal supplementation (Mohammed and Attaai, 2011). Because of the negative impacts of increased protein and energy in the diet on health and fertility (McEvoy et al, 1997;Dawuda et al, 2002) and the high cost of ration, it is important to search for non-traditional supplements with beneficial character-istics for body.…”

Aggregation-prone peptides modulate activity of bovine interferon gamma released from naturally occurring protein nanoparticles

“…Interestingly, recombinant products for animal health must not only be stable and safely and effectively delivered on a large scale and under standard conditions but also present obvious advantages over existing products to prove its commercial viability [47].…”

Engineered chromosome-based T7 RNA polymerase in Escherichia coli W3110 for orthogonal T7 promoter circuit as a cell factory

“…It is indispensable to develop the recombinant technology which enables enhanced enzyme expression to endorse high value chemicals production in recent years. The recombinant technology inspired the scienti c studies of enzymes, protein and protein interactions and the in vivo chemical production for industrial applications [1][2][3]. In addition, pharmaceutical and biotechnology companies applied recombinant technology to produce miscellaneous therapeutic proteins with the advantages of high output of the desired proteins and reduced cost due to the simpli ed puri cation processes.…”