Trehalose suppresses lipopolysaccharide-induced osteoclastogenesis bone marrow in mice

Trehalose Ingestion Modifies Mucosal Immune Responses of the Small Intestine in Mice.

“…10) Although the elevation of glucose and insulin in the serum has been found to diminish TNF-α production induced after LPS injection, 14) the levels of these parameters were within the normal range in the sera of mice administered trehalose just before LPS injection. We also reported that oral administration of trehalose decreased excess IL-6 secretion by bone marrow cells, leading to prevention of bone loss in OVX-mice.…”

“…9) In an endotoxin-induced bone resorption model in mice, trehalose significantly suppressed the elevation of serum tumor necrosis factor (TNF)-α induced by lipopolysaccharide (LPS)-injection, resulting in the inhibition of increased numbers of osteoclasts in the femurs. 10) However, we have not yet thoroughly established why trehalose ingestion leads to a down-regulation of the cytokines associated with bone resorption, such as IL-6 and TNF-α overexpressed in our osteoporosis models, nor have we identified the cells responsible for the changes in cytokine production.…”

“…1) This gave us the opportunity, to investigate the multiple functions of trehalose and to apply it in various areas such as 1) stabilization of protein and nucleic acids under dried conditions or at low temperatures, 2,3) 2) protection from stress induced by freezing, heating, and drying, [4][5][6][7] 3) prevention of osteoporosis. [8][9][10] As regards the third function, we have found that orally administered trehalose inhibited osteoclast differentiation in ovariectomized (OVX)-mice, 8) and showed that this effect was the result of inhibition of excess interleukin (IL)-6 production by bone marrow cells under conditions of estrogen-deficiency. 9) In an endotoxin-induced bone resorption model in mice, trehalose significantly suppressed the elevation of serum tumor necrosis factor (TNF)-α induced by lipopolysaccharide (LPS)-injection, resulting in the inhibition of increased numbers of osteoclasts in the femurs.…”

Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition

“…28 Moreover, trehalose was found to significantly suppress excessive increases in the quantity of osteoclasts stimulated by LPS. 29 Collectively, these findings suggest the feasibility of functionalizing TiO 2 nanotube surfaces by combining trehalose and lyophilization treatment of lentiviral vectors encoding BMP-2, to produce nanotube layers with enhanced osteogenic and anti-inflammatory properties.…”

“…Furthermore, a previous study also reported that trehalose suppresses osteoclast formation, which may be explained by the suppressive effects of trehalose on LPS-induced inflammatory cytokine production. 29 Although some researchers have studied their functional relationships, 28 the exact mechanism whereby trehalose suppresses inflammatory cytokine production remains unclear, and further studies on this subject are necessary. In comparison with the most common approaches for suppressing bacterial infections of implants using inorganic agents such as Cu, Zn, and Ag, 43 trehalose is a naturally occurring, nontoxic disaccharide that can preserve the activity of biomolecules and promote their sustained release from TiO 2 nanotubes.…”