Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition

Zhang, Xiaochen; Zhang, Zhiyuan; Shen, Gang; Zhao, Jun

doi:10.2147/ijn.s93177

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…In our study, BMP patients had lower mean total morphine usage (8 mg) in the immediate postoperative period when compared to the non-BMP group. One possible explanation may be an anti-inflammatory effect of BMP for these patients who were significantly older as well has having longer fusion levels [22]. At 6 months, patients in the BMP group had lower back and leg pain, which may be explained by earlier fusion in this group compared to the non-BMP group.…”

Section: Discussionmentioning

confidence: 99%

Bone Morphogenic Protein Is a Viable Adjunct for Fusion in Minimally Invasive Transforaminal Lumbar Interbody Fusion

et al. 2016

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Study DesignComparison of prospectively collected data of patients undergoing minimally invasive surgery transforaminal lumbar interbody fusion (MIS-TLIF) with and without recombinant human bone morphogenic protein 2 (BMP).PurposeTo compare the clinical, radiological outcome and complications of patients undergoing MIS-TLIF with and without BMP.Overview of LiteratureBMP is an effective fusion enhancer with potential complications. Direct comparison of MIS-TLIF with and without BMP is limited to retrospective studies with short follow-up.MethodsFrom June 2005 to February 2011, consecutive cases of MIS-TLIF performed by a single surgeon were included. North American Spine Society (NASS) score, Oswestry disability index (ODI), Short Form-36 (SF-36), and visual analogue score (VAS) were assessed preoperatively and at 6 and 24 months postoperatively. Fusion rates and complications were noted.ResultsThe 252 cases comprised 104 non-BMP and 148 BMP cases. The BMP group was significantly older (mean age, 60.2 vs. 53.9; p<0.01). Preoperative scores were similar. Immediate postoperative morphine usage was significantly lower in the BMP group (12.4 mg vs. 20.1 mg, p<0.01). At 6 months, the BMP group had lower VAS back and leg pain scores (p<0.01). At 2 years, the BMP group had better leg pain scores (p<0.01), ODI (15.4 vs. 20.3, p=0.04) and NASS scores (8.8 vs. 15.8, p<0.01). Both groups showed significant clinical improvement compared to their preoperative levels. The BMP group attained a significantly higher rate of fusion at 6 months follow-up (88.4% vs. 76.8%, p=0.016) with no difference at 2 years. The non-BMP and BMP group had 12 (11.5%) and 9 (6.1%) complications and 5 (4.8%) and 2 (1.4%) reoperations, respectively.ConclusionsThe use of BMP to augment fusion in MIS-TLIF is an acceptable alternative that has potential benefits of less pain in early and intermediate postoperative follow-up.

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Section: Discussionmentioning

confidence: 99%

Bone Morphogenic Protein Is a Viable Adjunct for Fusion in Minimally Invasive Transforaminal Lumbar Interbody Fusion

et al. 2016

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“…In line with this, Zhang et al [273,274] proposed a new strategy to functionalize TiO 2 NTs by loading a lentiviral vector encoding BMP2 (Lenti-BMP2) by lyophilization following trehalose addition. The obtained TiO 2 -Lyo-Tre-BMP2 nanoplatform ensured a slow and prolonged BMP2 release and promoted the highest extent osteogenic differentiation of bone marrow stromal cells as well as anti-inflammatory activity.…”

Section: In Vivo Drug Delivery Approaches Using Anodic Tio 2 Nanotube Implantsmentioning

confidence: 98%

Anodic TiO2 Nanotubes: Tailoring Osteoinduction via Drug Delivery

et al. 2021

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TiO2 nanostructures and more specifically nanotubes have gained significant attention in biomedical applications, due to their controlled nanoscale topography in the sub-100 nm range, high surface area, chemical resistance, and biocompatibility. Here we review the crucial aspects related to morphology and properties of TiO2 nanotubes obtained by electrochemical anodization of titanium for the biomedical field. Following the discussion of TiO2 nanotopographical characterization, the advantages of anodic TiO2 nanotubes will be introduced, such as their high surface area controlled by the morphological parameters (diameter and length), which provides better adsorption/linkage of bioactive molecules. We further discuss the key interactions with bone-related cells including osteoblast and stem cells in in vitro cell culture conditions, thus evaluating the cell response on various nanotubular structures. In addition, the synergistic effects of electrical stimulation on cells for enhancing bone formation combining with the nanoscale environmental cues from nanotopography will be further discussed. The present review also overviews the current state of drug delivery applications using TiO2 nanotubes for increased osseointegration and discusses the advantages, drawbacks, and prospects of drug delivery applications via these anodic TiO2 nanotubes.

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“…Oligonucleotide delivery to nuclei, found to be a cell division phase-dependent process, can happen during the interphase activity of cells, and the prophase condition mostly inhibits the internalization [176]. A sustained release of viral vector encoding proteins from the TiO 2 tubular implants can diminish disadvantages of local delivery systems (i.e., short effective time, large dose requirement, repetitive administration, and poor distribution) [177]. To overcome these disadvantages, lentiviral vectors encoding BMP-2 loaded into the nanotubes by the lyophilization were released over 8 days and promoted osteogenic differentiation.…”

Section: Dox-tio 2 Nts D Ox-tio 2 Ncsmentioning

confidence: 99%

“…This delivery system (lyophilization of the loaded vectors) has shown an advantage because of maintaining the stability of the vectors over the therapeutic period. Moreover, the sustained release of the lentiviral vector encoding BMP-2 improved the local cell accumulation 1 3 by recruiting circulating bone marrow stromal cells around the TiO 2 nanotubes and facilitated the differentiation into osteoblasts [177].…”

Section: Dox-tio 2 Nts D Ox-tio 2 Ncsmentioning

confidence: 99%

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Kafshgari

Goldmann

2020

Nano-Micro Lett.

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HIGHLIGHTS • Multifunctional TiO 2 nanostructures hold promise for advancing a wide range of biomedical applications due to a feasible integration of distinct theranostic features. • Fabrication and post-fabrication strategies implemented to generate multifunctional TiO 2 nanostructures for a broad range of biomedical applications are briefly outlined. The opportunities and challenges of TiO 2 nanomaterials are highlighted in order to open the possibility of clinical translation. ABSTRACT Titanium dioxide (TiO 2 ) nanostructures exhibit a broad range of theranostic properties that make them attractive for biomedical applications. TiO 2 nanostructures promise to improve current theranostic strategies by leveraging the enhanced quantum confinement, thermal conversion, specific surface area, and surface activity. This review highlights certain important aspects of fabrication strategies, which are employed to generate multifunctional TiO 2 nanostructures, while outlining post-fabrication techniques with anemphasis on their suitability for nanomedicine. The biodistribution, toxicity, biocompatibility, cellular adhesion, and endocytosis of these nanostructures, when exposed to biological microenvironments, are examined in regard to their geometry, size, and surface chemistry. The final section focuses on recent biomedical applications of TiO 2 nanostructures, specifically evaluating therapeutic delivery, photodynamic and sonodynamic therapy, bioimaging, biosensing, tissue regeneration, as well as chronic wound healing.

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Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition

Cited by 7 publications

References 47 publications

Bone Morphogenic Protein Is a Viable Adjunct for Fusion in Minimally Invasive Transforaminal Lumbar Interbody Fusion

Bone Morphogenic Protein Is a Viable Adjunct for Fusion in Minimally Invasive Transforaminal Lumbar Interbody Fusion

Anodic TiO2 Nanotubes: Tailoring Osteoinduction via Drug Delivery

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

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