Trehalose phosphate synthase overexpression in <i>Parachlorella kessleri</i> improves growth and photosynthetic performance under high light conditions

Rathod, Jayant Pralhad; Prakash, Gunjan; Vira, Chaitali; Lali, Arvind

doi:10.1080/10826068.2015.1135465

Cited by 15 publications

(14 citation statements)

References 32 publications

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“…In this study, we tried to detect some indexes that referred in plants under heat stress in P. pulmonarius. In addition, exogenous trehalose or overexpression of trehalose biosynthetic genes can improve abiotic stress resistance by increased trehalose accumulation in many organisms (Soto et al 1999;Garg et al 2002;Cortina and Culiáñez-Macià 2005;Kong et al 2012a;Yang et al 2014;Rathod et al 2016). The relationship between trehalose biosynthesis and thermotolerance, however, is not clear.…”

Section: Discussionmentioning

confidence: 99%

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

Liu

Gao

et al. 2019

Journal of Integrative Agriculture

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High temperature is one of the major abiotic stresses that limit edible mushroom growth and development. The understanding of physiological alterations in response to heat stress, and the corresponding mechanisms involved is vital for the breeding of heat-resistant edible mushroom strains. Although trehalose functions as a protectant against abiotic stresses in fungi, the putative role of trehalose in thermotolerance remains to be elucidated. In this study, we found heat stress inhibited the growth of two Pleurotus pulmonarius strains, heat-sensitive and less-sensitive, and the inhibition was more significant for the sensitive strain. Heat stress leads to the increase of lipid peroxidation and intracellular trehalose accumulation, with a higher level in the heat-sensitive strain, and this effect is independent of exogenous trehalose application. In addition, a lower concentration of exogenous trehalose application in sensitive strain than in less-sensitive strain was found to alleviate the inhibition of mycelium growth and further increase the intracellular trehalose concentration by heat stress. Thus, the protective effects of trehalose were more remarkable in the sensitive strain. The activities of intracellular trehalose metabolic enzymes, i.e, trehalose-6-phosphate synthase, trehalose phosphorylase and neutral trehalase, were determined, and our data indicated that the changes of these enzymes activities in the sensitive strain were more beneficial to accumulate trehalose than that in the less-sensitive strain.

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Section: Discussionmentioning

confidence: 99%

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

Liu

Gao

et al. 2019

Journal of Integrative Agriculture

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“…S1). The reactors were housed in a glass environmental chamber (Rathod et al ), which allowed study under direct sunlight along with diurnal variations (see Fig. S2).…”

Section: Methodsmentioning

confidence: 99%

Photosynthetic acclimation of Chlorella saccharophila to heat stress

Patil

Pandit

Lali

2017

Phycological Research

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Photosynthesis is one of the most important metabolic processes of algae; which is altered as a stress response. During mass cultivation of algae, temperature rise and high light are major factors that affect biomass productivity. High temperature affects photosystem II (PSII) complex irreversibly, damaging intermolecular interactions in it. However, the impact of high temperature on photosynthesis is highly variable among different algal species, depending on the prior acclimation to environmental conditions they were exposed to. The acclimation plays an important role in combating high temperature stress via regulation of photosynthetic responses. Chlorophyll a fluorescence is a highly sensitive, non-destructive and reliable tool for such measurements of photosynthetic parameters, which provides information about algal photosynthetic performance under given conditions. To understand the effect of heat stress on the responses of high light acclimated alga Chlorella saccharophila, chlorophyll a fluorescence transients were measured after heat exposure at 40 C. Our study demonstrates that rise in temperature for short duration; during open field cultivation reversibly affects the efficiency of PSII in light acclimated alga C. saccharophila. The effects of heat stress on chlorophyll a fluorescence in this alga, grown under high light (max-1600 μmol photons m −2 s −1 ) are presented here; they are used to infer changes in photosynthetic process during its exposure to heat, as well as their recovery after 72 h. We speculate that heat resistance may have been acquired due to prior exposures to high light.

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“…C. saccharophila and C. minutissima were cultivated in MB3N, whereas C. protothecoides in MN medium. The green alga Parachlorella kessleri was procured from the Indian Institute of Technology, Chennai, and grown in Walne's medium [7]. For experiment, the algal cultures were cultivated in 2,000 ml of polythene reactor in a specially designed environmental chamber (EC) (Fig.…”

Section: Algal Strains and Growth Conditionsmentioning

confidence: 99%

An efficient algae cell wall disruption methodology for recovery of intact chloroplasts from microalgae

2020

jabb

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The microalgal cell wall breakage has been identified as complex phenomenon which is highly dependent on the nature and composition of cell wall. A detailed analysis of plastids and their function requires the breaking open of cell without any damage to cellular components. To develop a rapid and universal methodology for cell wall breakage, liquid nitrogen crushing, sonication, enzymatic lysis, and homogenization procedures were applied to various microalgal species. Homogenization-based procedure for the isolation of intact chloroplast was found to be universal for all algal species under the study. The isolated chloroplasts were subjected to chloroplast integrity analysis. The intact chloroplast exhibited a positive maximum quantum yield and F v / F m values ranging from 0.1 to 0.4 as measured by pulse amplitude modulation fluorometry and was found to be suitable for further downstream applications such as isolation of protein-pigment complexes involved in photosynthetic O 2 evolution. The developed methodology is a quick and efficient technique for the isolation of intact chloroplasts across different genera of microalgae by employing minor changes in the base protocol as a species-specific characteristic.

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Trehalose phosphate synthase overexpression in Parachlorella kessleri improves growth and photosynthetic performance under high light conditions

Cited by 15 publications

References 32 publications

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

Photosynthetic acclimation of Chlorella saccharophila to heat stress

An efficient algae cell wall disruption methodology for recovery of intact chloroplasts from microalgae

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