“…Heat treatment could also affect the intracellular and extracellular metabolites of mushroom mycelia, such as ganoderic acid and volatile components (Tereshina et al, 2011;Liu et al, 2018c;Zhang et al, 2019). To counteract the detrimental effects of heat, mushroom cells evolved multiple adaption systems by changing proteins expression including (1) heat stress signal responding transduction system (Guo et al, 2016;Loshchinina and Nikitina, 2016;Zhang et al, 2016;Liu et al, 2018b); (2) the induction of heat stress factors and heat shock proteins (HSPs) (Lee et al, 2006;Kurahashi et al, 2014;Zhang et al, 2016;Chen et al, 2017;Wang et al, 2018a); (3) ROS burst and significant antioxidant accumulations (Chen et al, 2017;Wang et al, 2017;Liu et al, 2018c); (4) anabolism inhibition and catabolism activation, such as trehalose accumulation (Kong et al, 2012b;Liu et al, 2016Liu et al, , 2019Chen et al, 2017). Recently, the protection effect of antioxidant kojic acid in H. marmoreus mycelia damage was studied (Zhang et al, 2017).…”