Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Abstract

The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred to tobacco (Nicotiana tabacum) to evaluate its function in thermotolerance. Sequence alignments and phylogenetic analysis revealed that HmHsp70 may be located in the mitochondria region. qPCR analysis revealed that the transcription level of hmhsp70 in H. marmoreus mycelia increased after heat shock treatment in high temperature (42 °C) compared with untreated mycelia (at 25 °C). Transgenic tobaccos expressing hmhsp70 gene showed enhanced resistance to lethal temperature compared with the wild type (WT) plants. Nearly 30% of the transgenic tobaccos survived after treated at a high temperature (50 °C and 52 °C for 4 h); however, almost all the WT tobaccos died after treated at 50 °C and no WT tobacco survived after heat shock at 52 °C. This study firstly showed the function of a hsp70 gene from H. marmoreus.

Label-Free Comparative Proteomics Analysis Revealed Heat Stress Responsive Mechanism in Hypsizygus marmoreus

“…In our previous report, one Hsp70 (A0A369K2K3) gene, hmHsp70, was cloned from H. marmoreus and cloned into tobacco. The transgenic tobacco showed enhanced resistance to lethal temperature (Xu et al, 2020). Similar result was observed in this study, the expression of HmHsp70 increased by 8.805 fold and then decreased to 0.747 fold after recovery cultivation.…”

Meta-Analysis as a Tool to Identify Candidate Genes Involved in the Fagus sylvatica L. Abiotic Stress Response

“…In plants, it has been observed that initial exposure from medium to high heat stress provides resistance against a subsequent lethal dose. This phenomenon is called acquired tolerance [38,39]. The heat stress response is characterised by an elevated synthesis of a specific set of heat shock proteins (Hsps) associated with the development of thermotolerance [40][41][42].…”

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“…Nevertheless, the signal transduction processes and the regulation mechanism of HSPs in H. marmoreus under heat stress remain unclear. The key proteins identified by proteomics analysis have been successfully used to rebuild and enhance the thermotolerance of mushrooms (Wang et al, 2018b;Xu et al, 2020). Therefore, this study may be helpful for the breeding of heat-resistance H. marmoreus strains.…”

“…strain G12 used in this study was provided by Shandong Provincial Key Laboratory of Applied Mycology. H. marmoreus mycelia were cultivated in potato dextrose agar (PDA: extract from 200 g potato, 20 g dextrose, 20 g agar) plate at 25 • C in darkness as previously described (Wang et al, 2019;Xu et al, 2020). A total of 60 plates were divided randomly into three groups: Con group, HS group, and RHS group.…”

“…H. marmoreus is a low-temperature fruiting mushroom. The mycelia grow vegetatively at 23 • C to 25 • C and transform into fruiting process at 13 • C to 17 • C. The fruiting process of H. marmoreus is sensitive to temperature, and high temperature can lead to the death of mycelia and malformation of the fruiting body (Zhang et al, 2019;Xu et al, 2020). Therefore, thermotolerance is important for H. marmoreus during both vegetative growth and fruiting-body formation.…”