Trehalose-Modified Silver Nanoparticles as Antibacterial Agents with Reduced Cytotoxicity and Enhanced Uptake by Mycobacteria

Wijesundera, Samurdhi A.; Jayawardana, Kalana W.; Yan, Mingdi

doi:10.1021/acsanm.2c02047

Cited by 12 publications

(12 citation statements)

References 56 publications

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“…Tre-Cz was prepared by coupling the amine-derivatized trehalose V (Scheme S1) − with the N -hydroxysuccinimide (NHS)-functionalized carbazole IV (Scheme S3). , The reaction was carried out at room temperature, giving Tre-Cz in 60% yield (Scheme ).…”

Section: Results and Discussionmentioning

confidence: 99%

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Liyanage

Raviranga

Ryan

et al. 2023

JACS Au

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A fluorescence turn-on probe, an azide-masked and trehalose-derivatized carbazole (Tre-Cz), was developed to image mycobacteria. The fluorescence turn-on is achieved by photoactivation of the azide, which generates a fluorescent product through an efficient intramolecular C–H insertion reaction. The probe is highly specific for mycobacteria and could image mycobacteria in the presence of other Gram-positive and Gram-negative bacteria. Both the photoactivation and detection can be accomplished using a handheld UV lamp, giving a limit of detection of 103 CFU/mL, which can be visualized by the naked eye. The probe was also able to image mycobacteria spiked in sputum samples, although the detection sensitivity was lower. Studies using heat-killed, stationary-phase, and isoniazid-treated mycobacteria showed that metabolically active bacteria are required for the uptake of Tre-Cz. The uptake decreased in the presence of trehalose in a concentration-dependent manner, indicating that Tre-Cz hijacked the trehalose uptake pathway. Mechanistic studies demonstrated that the trehalose transporter LpqY-SugABC was the primary pathway for the uptake of Tre-Cz. The uptake decreased in the LpqY-SugABC deletion mutants ΔlpqY, ΔsugA, ΔsugB, and ΔsugC and fully recovered in the complemented strain of ΔsugC. For the mycolyl transferase antigen 85 complex (Ag85), however, only a slight reduction of uptake was observed in the Ag85 deletion mutant ΔAg85C, and no incorporation of Tre-Cz into the outer membrane was observed. The unique intracellular incorporation mechanism of Tre-Cz through the LpqY-SugABC transporter, which differs from other trehalose-based fluorescence probes, unlocks potential opportunities to bring molecular cargoes to mycobacteria for both fundamental studies and theranostic applications.

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Section: Results and Discussionmentioning

confidence: 99%

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Liyanage

Raviranga

Ryan

et al. 2023

JACS Au

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“… 55 Also, the functionalization of nanomaterials has been proven to be useful for the reduced cytotoxicity of nanomaterials without the hindrance of their beneficial effects. 56 Additionally, both encapsulation and functionalization could endow the nanomaterials with new properties, further broadening the practical application of the materials. Moreover, since silver has been widely applied in the biomedical field, such as antimicrobial agents, drug-delivery carriers, medical device coatings, tissue regeneration, and so on, 57 we supposed that Ag/Ag 2 O could find a way to optimize its biocompatibility for better biomedical application.…”

Section: Resultsmentioning

confidence: 99%

Ag/Ag2O with NIR-Triggered Antibacterial Activities: Photocatalytic Sterilization Enhanced by Low-Temperature Photothermal Effect

Qian¹,

Zhao²,

Hu³

et al. 2023

IJN

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Purpose A synergistic antibacterial system employing photocatalytic performance and low-temperature photothermal effect (LT-PTT) with the potential for infectious skin wound healing promotion was developed. Methods Ag/Ag 2 O was synthesized with a two-step method, and its physicochemical properties were characterized. After its photocatalytic performance and photothermal effect were evaluated under 0.5 W/cm 2 808 nm NIR laser irradiation, its antibacterial activities in both planktonic and biofilm forms were then studied in vitro targeting Staphylococcus Aureus (S. aureus) , and the biocompatibility was tested with L-929 cell lines afterward. Finally, the animal model of dorsal skin wound infection was established on Sprague-Dawley rats and was used to assess infectious wound healing promotion of Ag/Ag 2 O in vivo. Results Ag/Ag 2 O showed boosted photocatalytic performance and local temperature accumulation compared with Ag 2 O when exposed to 0.5 W/cm 2 808 nm NIR irradiation, which therefore endowed Ag/Ag 2 O with the ability to kill pathogens rapidly and cleavage bacterial biofilm in vitro. Furthermore, after treatment with Ag/Ag 2 O and 0.5 W/cm 2 808 nm NIR irradiation, infectious wounds of rats realized skin tissue regeneration from a histochemical level. Conclusion By exhibiting excellent NIR-triggered photocatalytic sterilization ability enhanced by low-temperature photothermal effect, Ag/Ag 2 O was promising to be a novel, photo-responsive antibacterial agent.

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“…Ongoing work focuses on optimization of AuNC uptake into bacteria by capping AuNCs with bacterium-specific ligands that enhance the interactions with the target bacterium. [71][72][73]…”

Section: Discussionmentioning

confidence: 99%

Turning on the Antimicrobial Activity of Gold Nanoclusters Against Multidrug‐Resistant Bacteria**

et al. 2023

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In this work, we show that the addition of thiourea (TU) initiated broad-spectrum antimicrobial activity of otherwise inactive D-maltose-capped gold nanoclusters (AuNC-Mal). For example, AuNC-Mal/TU was effective against multidrug-resistant Pseudomonas aeruginosa with a minimum inhibitory concentration (MIC) of 1 μg mL À 1 (2.5 μM [Au]) while having 30-60 times lower in vitro cytotoxicity against mammalian cells. The reaction of AuNC-Mal and TU generated the antimicrobial species of [Au(TU) 2 ] + and smaller AuNCs. TU increased the accumulation of Au in bacteria and helped maintain the oxidation state as Au I (vs. Au III ). The modes of action included the inhibition of thioredoxin reductase, interference with the Cu I regulation and depletion of ATP. Moreover, the antimicrobial activity did not change in the presence of colistin or carbonyl cyanide 3-chlorophenylhydrazone, suggesting that AuNC-Mal/TU was indifferent to the outer membrane barrier and to bacterial efflux pumps.

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Trehalose-Modified Silver Nanoparticles as Antibacterial Agents with Reduced Cytotoxicity and Enhanced Uptake by Mycobacteria

Cited by 12 publications

References 56 publications

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Ag/Ag2O with NIR-Triggered Antibacterial Activities: Photocatalytic Sterilization Enhanced by Low-Temperature Photothermal Effect

Turning on the Antimicrobial Activity of Gold Nanoclusters Against Multidrug‐Resistant Bacteria**

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