Turning on the Antimicrobial Activity of Gold Nanoclusters Against Multidrug‐Resistant Bacteria**

Ndugire, William; Truong, Dang; Raviranga, Hasitha; Lao, Jingzhe; Ramström, Olof; Yan, Mingdi

doi:10.1002/anie.202214086

Cited by 9 publications

(9 citation statements)

References 76 publications

(157 reference statements)

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“… , The reaction was carried out at room temperature, giving Tre-Cz in 60% yield (Scheme ). The corresponding Mal-Cz was synthesized in the same manner as Tre-Cz by coupling IV with an amine-derivatized Mal ( VI , Scheme S2) − in 73% yield (Scheme ). The structure of Tre-Cz was confirmed by 1 H NMR (Figures S50 and S51), IR (Figure S52), 19 F NMR (Figure S53), 13 C NMR (Figure S54), 2D COSY NMR (Figure S55), and high-resolution mass spectrometry (Figure S56).…”

Section: Results and Discussionmentioning

confidence: 99%

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Liyanage

Raviranga

Ryan

et al. 2023

JACS Au

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A fluorescence turn-on probe, an azide-masked and trehalose-derivatized carbazole (Tre-Cz), was developed to image mycobacteria. The fluorescence turn-on is achieved by photoactivation of the azide, which generates a fluorescent product through an efficient intramolecular C–H insertion reaction. The probe is highly specific for mycobacteria and could image mycobacteria in the presence of other Gram-positive and Gram-negative bacteria. Both the photoactivation and detection can be accomplished using a handheld UV lamp, giving a limit of detection of 103 CFU/mL, which can be visualized by the naked eye. The probe was also able to image mycobacteria spiked in sputum samples, although the detection sensitivity was lower. Studies using heat-killed, stationary-phase, and isoniazid-treated mycobacteria showed that metabolically active bacteria are required for the uptake of Tre-Cz. The uptake decreased in the presence of trehalose in a concentration-dependent manner, indicating that Tre-Cz hijacked the trehalose uptake pathway. Mechanistic studies demonstrated that the trehalose transporter LpqY-SugABC was the primary pathway for the uptake of Tre-Cz. The uptake decreased in the LpqY-SugABC deletion mutants ΔlpqY, ΔsugA, ΔsugB, and ΔsugC and fully recovered in the complemented strain of ΔsugC. For the mycolyl transferase antigen 85 complex (Ag85), however, only a slight reduction of uptake was observed in the Ag85 deletion mutant ΔAg85C, and no incorporation of Tre-Cz into the outer membrane was observed. The unique intracellular incorporation mechanism of Tre-Cz through the LpqY-SugABC transporter, which differs from other trehalose-based fluorescence probes, unlocks potential opportunities to bring molecular cargoes to mycobacteria for both fundamental studies and theranostic applications.

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Section: Results and Discussionmentioning

confidence: 99%

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Liyanage

Raviranga

Ryan

et al. 2023

JACS Au

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“…, 24, 10006 10 of 21 proven through multiple modes of actions in bacteria, such as inhibiting the TrxR enzyme, depleting ATP, and interfering with copper regulation [90]. Enhanced ROS generation by AuNCs and the overcoming of poor drug penetration can be induced by light irradiation.…”

Section: Antibacterial Auncs For Mdr Bacteriamentioning

confidence: 99%

“…Furthermore, AuNCs have exhibited antibacterial mechanisms of photodynamic therapy (PDT) and photothermal therapy (PTT). Therefore, AuNCs have strong antibacterial activity against a wide range of bacterial strains, including both gram-positive and gram-negative bacteria, MDR bacteria, and even bacterial biofilms [ 90 , 91 , 92 , 93 , 94 ].…”

Section: Antibacterial Nanostructures Of Auncsmentioning

confidence: 99%

“…In addition, the gram-positive bacterium, Clostridium difficile , was inhibited by MHA-AuNCs. MHA-AuNCs (100 µM) generated a 5-fold increase in ROS levels, which drastically killed C. difficile by destroying membrane integrity without toxic effects on human cells [ 90 ]. Not only MDR gram-positive strains can be eradicated when interacting with AuNCs, but also MDR gram-negative strains.…”

Section: Antibacterial Nanostructures Of Auncsmentioning

confidence: 99%

“…AuNC-Mal/TU effectively inhibited MDR P. aeruginosa after 15 min of incubation. The antibacterial mechanisms of AuNC-Mal/TU were proven through multiple modes of actions in bacteria, such as inhibiting the TrxR enzyme, depleting ATP, and interfering with copper regulation [ 90 ]. Enhanced ROS generation by AuNCs and the overcoming of poor drug penetration can be induced by light irradiation.…”

Section: Antibacterial Nanostructures Of Auncsmentioning

confidence: 99%

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Gold-Based Nanostructures for Antibacterial Application

Mutalik

Saukani

Khafid

et al. 2023

IJMS

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Bacterial infections have become a fatal threat because of the abuse of antibiotics in the world. Various gold (Au)-based nanostructures have been extensively explored as antibacterial agents to combat bacterial infections based on their remarkable chemical and physical characteristics. Many Au-based nanostructures have been designed and their antibacterial activities and mechanisms have been further examined and demonstrated. In this review, we collected and summarized current developments of antibacterial agents of Au-based nanostructures, including Au nanoparticles (AuNPs), Au nanoclusters (AuNCs), Au nanorods (AuNRs), Au nanobipyramids (AuNBPs), and Au nanostars (AuNSs) according to their shapes, sizes, and surface modifications. The rational designs and antibacterial mechanisms of these Au-based nanostructures are further discussed. With the developments of Au-based nanostructures as novel antibacterial agents, we also provide perspectives, challenges, and opportunities for future practical clinical applications.

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Gold nanoclusters encapsulated microneedle patches with antibacterial and self‐monitoring capacities for wound management

Yi,

Yu,

Fan

et al. 2024

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The management of infected wounds is always of great significance and urgency in clinical and biomedical fields. Recent efforts in this area are focusing on the development of functional wound patches with effective antibacterial, drug delivery, and sensor properties. Here, we present novel hyaluronic acid (HA) microneedle patches with these features by encapsulating aminobenzeneboronic acid‐modified gold nanoclusters (A‐GNCs) for infected wound management. The A‐GNCs loaded microneedle patches were derived from negative‐mold replication and showed high mechanical strength to penetrate the skin. The release of the A‐GNCs was realized by the degradation of HA, and the self‐monitor of the released actives was based on the dynamic bright orange fluorescence emitted from A‐GNCs under ultraviolet radiation. As the A‐GNCs could destroy bacteria membranes, the microneedle patches were with excellent in vitro antibiosis ability. Based on these features, we have demonstrated the bacteria inhibition, residual drug self‐monitoring, and wound healing promotion abilities of the microneedle patches in Escherichia coli‐ or Staphylococcus aureus‐infected wound management. These results indicated the great potential of such A‐GNCs loaded microneedle patches for clinical applications.

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Turning on the Antimicrobial Activity of Gold Nanoclusters Against Multidrug‐Resistant Bacteria**

Cited by 9 publications

References 76 publications

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria

Gold-Based Nanostructures for Antibacterial Application

Gold nanoclusters encapsulated microneedle patches with antibacterial and self‐monitoring capacities for wound management

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