Trefoil factors

Baus-Lončar, Mirela; Giraud, Andrew S.

doi:10.1007/s00018-005-5480-x

Cited by 56 publications

(24 citation statements)

References 117 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The interferon regulatory factor (IRF)-1 is also an immune active molecule and inflammatory process regulator, the activation of IRF-1 and NF-κB was found to be concurrently activated in melanoma [27]. In addition, polymorphisms of the trefoil factor 3( TFF3 ) promoter were associated with gastric cancer susceptibility [28] and TFF3 was regulated by both HIF-1 and NFκB [29]. Overexpression of TFF3 was an independent indicator for overall survival of gastric cancer patients [30].…”

Section: Resultsmentioning

confidence: 99%

Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

Wang

Duan

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Tissue hypoxia induces reprogramming of cell metabolism and may result in normal cell transformation and cancer progression. Hypoxia-inducible factor 1-alpha (HIF-1α), the key transcription factor, plays an important role in gastric cancer development and progression. This study aimed to investigate the underlying regulatory signaling pathway in gastric cancer using gastric cancer tissue specimens. The integration of gene expression profile and transcriptional regulatory element database (TRED) was pursued to identify HIF-1α ↔ NFκB1 → BRCA1 → STAT3 ← STAT1 gene pathways and their regulated genes. The data showed that there were 82 differentially expressed genes that could be regulated by these five transcription factors in gastric cancer tissues and these genes formed 95 regulation modes, among which seven genes (MMP1, TIMP1, TLR2, FCGR3A, IRF1, FAS, and TFF3) were hub molecules that are regulated at least by two of these five transcription factors simultaneously and were associated with hypoxia, inflammation, and immune disorder. Real-Time PCR and western blot showed increasing of HIF-1α in mRNA and protein levels as well as TIMP1, TFF3 in mRNA levels in gastric cancer tissues. The data are the first study to demonstrate HIF-1α-regulated transcription factors and their corresponding network genes in gastric cancer. Further study with a larger sample size and more functional experiments is needed to confirm these data and then translate into clinical biomarker discovery and treatment strategy for gastric cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

Wang

Duan

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Studies of corresponding enhancer regions or nuclear proteins in non-intestinal TFF3-expressing tissues have not been published. Thus, different tissues may have individual mechanisms for the regulation of TFF expression, as seen for TFF1, which is upregulated in response to estrogen in the mammary gland but not in the stomach [14,78]. One way the tissue-specific expression of the individual TFFs seems to be regulated is by epigenetic regulation, e.g.…”

Section: Regulation Of Expressionmentioning

confidence: 96%

“…What drives the expression of the TFFs is not yet fully understood, but several mechanisms seem to be involved (reviewed in [132,133,78]). This section will address the following issues: 1) How is the strict tissue-specific and often complementary expression of TFFs in normal tissues, achieved?…”

Section: Regulation Of Expressionmentioning

confidence: 99%

“…Outside the GI tract, varying levels of TFF expression have been described, especially in tissues containing mucus-secreting cells, i.e. respiratory and ocular tissues [49, 52, 73 -76], salivary glands [52,53,70], prostate [52] and female reproductive organs [52,54,77,78] as well as in milk [79]. Interestingly, marked fluctuations in the TFF3 serum levels have been noted during pregnancy [80].…”

Section: Tissue Expression and Detection In Fluidsmentioning

confidence: 99%

See 1 more Smart Citation

The trefoil factor family – small peptides with multiple functionalities

Kjellev¹

2008

Cell. Mol. Life Sci.

230

252

View full text Add to dashboard Cite

The trefoil factor family (TFF) comprises a group of small peptides which are highly expressed in tissues containing mucus-producing cells - especially in the mucosa lining the gastrointestinal tract. The peptides seem crucial for epithelial restitution and may work via other pathways than the conventional factors involved in restitution. In vitro studies have shown that the TFFs promote restitution using multiple mechanisms. The peptides also have other functionalities including interactions with the immune system. Moreover, therapeutic effects of the TFFs have been shown in several animal models of gastrointestinal damage. Still it is not clear which of their in vitro properties are involved in the in vivo mode of action. This review describes the TFF family with emphasis on their biological properties and involvement in mucosal protection and repair.

show abstract

“…Dense methylation of DNA has been associated with transcriptional repression of chromatin characterized by under-acetylated histones [35]. On the other hand, histone acetyltransferases acetylate histones and cause a weakening of the bonds between histones and DNA, promoting exposure of DNA to the transcriptional machinery [36]. Thus, independent of alterations in the DNA sequence, epigenetic mechanisms enable transient response to environmental variance through changes in gene expression [34].…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Control of Trefoil Factor Family (TFF) Peptide Expression in Human Retinoblastoma Cell Lines

Philippeit¹,

Busch²,

Dünker³

2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Recent studies demonstrated that epigenetic mechanisms are involved in the regulation of trefoil factor family (TFF) peptide expression in cancer. In human tissues with endogenous TFF1, TFF2 or TFF3 gene expression, the corresponding promoter is unmethylated and in organs without TFF expression, the promoter of the three genes is highly methylated. Methods: Retinoblastoma (Rb) cell lines were treated with the DNA methyltransferase inhibitor 5-Aza-2`deoxycytidine (5-Aza-dC), the histone deacetylase inhibitor 4-Phenylbutyric acid (PBA) or both and analyzed for changes (i) in TFF mRNA expression by Real-time PCR and (ii) in the methylation status of the TFF promoters by genomic bisulfite sequencing. Results: The degree of promoter methylation correlates with endogenous TFF expression in the retinoblastoma cell lines analyzed. Nearly all Rb cell lines exhibiting high endogenous TFF1 expression displayed low methylation of the CpGs in the corresponding promoter region. Low expression of TFF3 in Rb cell lines is linked with high density methylation of the TFF3 promoter. 5-Aza-dC treatment induced TFF1 and TFF3 expression in nearly all cell lines investigated and combined treatment with PBA further increased this effect. The number of methylated CpG dinucleotides of the TFF promoter is clearly reduced upon treatment with 5-Aza-dC and combined treatment with PBA further extended the degree of demethylation. Conclusion: Our data clearly show that the expression of TFF3 in retinoblastoma cell lines is epigenetically regulated, whereas the level of TFF1 and TFF2 seems to be regulated by other or additional mechanisms.

show abstract

Trefoil factors

Cited by 56 publications

References 117 publications

Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

The trefoil factor family – small peptides with multiple functionalities

Epigenetic Control of Trefoil Factor Family (TFF) Peptide Expression in Human Retinoblastoma Cell Lines

Contact Info

Product

Resources

About