Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

Wang, Jihan; Ni, Zhaohui; Duan, Zipeng; Wang, Guoqing; Фан, Ли

doi:10.1371/journal.pone.0099835

Cited by 52 publications

(44 citation statements)

References 30 publications

(34 reference statements)

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“…Although no definite receptor or binding molecule has been identified, it has been hypothesized that TFF3 may act in a receptor‐mediated manner . The HK‐2 cultured human cell line (proximal tubular epithelial cells) can synthesize and excrete more TFF3 after exposure to damage such as nutrient starvation, hypoxia and immunoglobulin λ light chain . Hypoxia induces the transcription of HIF‐1 and TFF3 mRNA in vitro, and it is known that TFF3 is regulated by HIF‐1α .…”

Section: Discussionmentioning

confidence: 99%

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy

et al. 2018

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AimTrefoil factor 3 (TFF3) is a small peptide that is involved in mucosal protection. TFF3 is widely expressed in multiple tissues including kidney tissue. Previous studies have reported that the levels of urinary TFF3 are significantly increased in patients with chronic kidney disease. The aim of this study is to detect the TFF3 mRNA in kidney and elucidate the relationship between renal TFF3 mRNA and tubulointerstitial fibrosis in IgA nephropathy (IgAN).MethodsWe investigated the renal mRNA expression of TFF3 by real‐time PCR analysis in biopsy specimens from patients with IgAN, other glomerulonephritis (OGN) and minor glomerular abnormalities (MGA). We also determined the renal localization of TFF3 and the levels of urinary TFF3 by immunostaining and ELISA, respectively.ResultsThe renal TFF3 mRNA expression was significantly associated with the urinary TFF3 secretion and the tubulointerstitial fibrosis score in the IgAN group alone. Immunostaining of the renal specimen of IgAN patients revealed that TFF3 is located in the renal tubular epithelial cells. The locations were almost the same as those that showed uromodulin positivity; specifically, the thick ascending limb (TAL) of the loop of Henle and the early portion of the distal tubule. The urinary TFF3 levels were positively correlated with the levels of urinary biomarkers of tubulointerstitial injury in such patients.ConclusionRenal TFF3 mRNA is associated with renal tubulointerstitial fibrosis in IgAN patients. The TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgAN patients.

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Section: Discussionmentioning

confidence: 99%

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy

et al. 2018

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“…To identify DEGs in Ewing sarcoma after EWSR1/FLI1 knockdown, we utilized the limma algorithm package in R/Bioconductor to identify differentially expressed genes between two groups at a time [15], [16]. The dataset on 0 h was used as the control group and we compared the other five time points after EWSR1/FLI1 knockdown to the 0 h control.…”

Section: Methodsmentioning

confidence: 99%

“…The TRED (http://rulai.cshl.edu/TRED) provides an accurate and comprehensive knowledge involves transcriptional regulatory elements [16], [17]. Thus, we first obtained all the transcription factor-gene regulation modes on 36 cancer-related TF families from TRED, then constructed the dysregulated TF-genes regulatory network by integrating DEGs with transcriptional regulation modes.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, we first obtained all the transcription factor-gene regulation modes on 36 cancer-related TF families from TRED, then constructed the dysregulated TF-genes regulatory network by integrating DEGs with transcriptional regulation modes. The Cytoscape software was utilized to visualize such regulatory networks [16], [18]. For example, triangles in the network are TFs (orange for up-regulated, yellow for down-regulated), circles in the network are target genes (red for up-regulated and green for down-regulated) in A673 Ewing sarcoma cells after EWSR1/FLI1 knockdown.…”

Section: Methodsmentioning

confidence: 99%

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Knockdown of EWSR1/FLI1 expression alters the transcriptome of Ewing sarcoma cells in vitro

Wang

Jiang

Yan

et al. 2016

Journal of Bone Oncology

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Ewing sarcoma breakpoint region 1 (EWSR1) fusion with Friend leukemia integration 1 transcription factor (FLI1) induced by a translocation of chromosome 11 with 22 contributes to Ewing sarcoma development. To date, the precise molecular mechanisms about EWSR1/FLI1 involving in Ewing sarcoma development remains to be defined. This study explored the potential critical gene targets of EWSR1/FLI1 knockdown in Ewing sarcoma cells on the gene expression profile based on online dataset, performed Limma algorithm for differentially expressed genes identification, constructed the transcriptional factor (TF)-gene regulatory network based on integrate transcriptional regulatory element database (TRED). The data showed up- and down-regulation of differentially expressed genes over time and peaked at 72 h after EWSR1/FLI1 knockdown in Ewing sarcoma cells. SMAD3 were up-regulated and FLI1, MYB, E2F1, ETS2, WT1 were down-regulated with more than half of their targets were down-regulated after EWSR1/FLI1 knockdown. The Gene Ontology (GO) and pathway annotation of these differentially expressed genes showed a consistent trend in each group of samples. Totally, there were 355 differentially expressed genes occurring in all five comparison groups of different time points, in which 39 genes constructed a dysregulated TF-gene network in Ewing sarcoma cell line A673 after EWSR1/FLI1 knockdown. These data demonstrated that knockdown of EWSR1/FLI1 expression led to transcriptome changes in Ewing sarcoma cells and that Ewing sarcoma development and progression caused by altered EWSR1/FLI1 expression may be associated with more complex transcriptome changes.

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“…To obtain high yields of a recombinant protein, the protein-coding gene is usually expressed at the highest possible level (13), so improving the involved factors in gene expression can be useful. A few studies have employed qRT-PCR to study the effects of the involved factors in gene expression in eukaryotic cells (14, 15). For the first time, we utilized this technique to evaluate effective factors in the expression of synthetic hES gene, which is involved in the synthesis of rhES protein.…”

Section: Discussionmentioning

confidence: 99%

Effect of Culture Condition Variables on Human Endostatin Gene Expression in Escherichia coli Using Response Surface Methodology

Mohajeri

Pilehvar-Soltanahmadi

Abdolalizadeh

et al. 2016

Jundishapur J Microbiol

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BackgroundRecombinant human endostatin (rhES) is an angiogenesis inhibitor used as a specific drug for the treatment of non-small-cell lung cancer. As mRNA concentration affects the recombinant protein expression level, any factor affecting mRNA concentration can alter the protein expression level. Response surface methodology (RSM) based on the Box-Behnken design (BBD) is a statistical tool for experimental design and for optimizing biotechnological processes.ObjectivesThis investigation aimed to predict and develop the optimal culture conditions for mRNA expression of the synthetic human endostatin (hES) gene in Escherichia coli BL21 (DE3).Materials and MethodsThe hES gene was amplified, cloned, and expressed in the E. coli expression system. Three factors, including isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration, post-induction time, and cell density before induction, were selected as important factors. The mRNA expression level was determined using real-time PCR. The expression levels of hES mRNA under the different growth conditions were analyzed. SDS-PAGE and western blot analyses were carried out for further confirmation of interest-gene expression.ResultsA maximum rhES mRNA level of 376.16% was obtained under the following conditions: 0.6 mM IPTG, 7 hours post-induction time, and 0.9 cell density before induction. The level of rhES mRNA was significantly correlated with post-induction time, IPTG concentration, and cell density before induction (P < 0.05). The expression of the hES gene was confirmed by western blot.ConclusionsThe obtained results indicate that RSM is an effective method for the optimization of culture conditions for hES gene expression in E. coli.

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Altered Expression of Hypoxia-Inducible Factor-1α (HIF-1α) and Its Regulatory Genes in Gastric Cancer Tissues

Cited by 52 publications

References 30 publications

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy

Knockdown of EWSR1/FLI1 expression alters the transcriptome of Ewing sarcoma cells in vitro

Effect of Culture Condition Variables on Human Endostatin Gene Expression in Escherichia coli Using Response Surface Methodology

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