Treatment with paeoniflorin increases lifespan of Pseudomonas aeruginosa infected Caenorhabditis elegans by inhibiting bacterial accumulation in intestinal lumen and biofilm formation

Wang, Yuxing; Zhang, Le; Yuan, Xiaoan; Wang, Dayong

doi:10.3389/fphar.2023.1114219

Cited by 9 publications

(9 citation statements)

References 90 publications

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“…Recognized as one of the most formidable nosocomial pathogens, the treatment of P. aeruginosa infection poses a challenge due to the restricted array of available antibiotics ( Bassetti et al, 2018 ). C. elegans has established itself as a robust invertebrate model for investigating host-pathogen interactions ( Aballay and Ausubel, 2002 ; Yu et al, 2018 ; Martineau et al, 2021 ; Wang et al, 2023 ). CIEO and its main component, CID, have been confirmed to possess various biological activities, including antiplatelet activity, anti-hyperglycemic and lipid-lowering applications, inhibition of cancer cell proliferation, and antibacterial properties ( Ka et al, 2003 ; Tognolini et al, 2006 ; Subash Babu et al, 2007 ; Lu et al, 2020 ; Abeysekera et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Post-seeding, the lethality-inducing plates underwent incubation at 37°C for a duration of 24 h, followed by an additional 24 h of incubation at 25°C. Young nematodes were translocated onto lethality-inducing plates to commence P. aeruginosa PA14 infection, maintained at 25°C for a duration of 24 h ( Wang et al, 2023 ). Three independent replicates were executed.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial activity of cinnamon essential oil and its main component of cinnamaldehyde and the underlying mechanism

Shu,

Ge,

et al. 2024

Front. Pharmacol.

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Background: Plant essential oils have long been regarded as repositories of antimicrobial agents. In recent years, they have emerged as potential alternatives or supplements to antimicrobial drugs. Although literature reviews and previous studies have indicated that cinnamon essential oil (CIEO) and its major component, cinnamaldehyde (CID), possess potent antibacterial activities, their antibacterial mechanisms, especially the in vivo antibacterial mechanisms, remain elusive.Methods: In this study, we utilized the in vivo assessment system of Caenorhabditis elegans (C. elegans) to investigate the effects and mechanisms of high dose (100 mg/L) and low dose (10 mg/L) CIEO and CID in inhibiting Pseudomonas aeruginosa (P. aeruginosa). In addition, we also examined the in vitro antibacterial abilities of CIEO and CID against other common pathogens including P. aeruginosa and 4 other strains.Results: Our research revealed that both high (100 mg/L) and low doses (10 mg/L) of CIEO and CID treatment significantly alleviated the reduction in locomotion behavior, lifespan, and accumulation of P. aeruginosa in C. elegans infected with the bacteria. During P. aeruginosa infection, the transcriptional expression of antimicrobial peptide-related genes (lys-1 and lys-8) in C. elegans was upregulated with low-dose CIEO and CID treatment, while this trend was suppressed at high doses. Further investigation suggested that the PMK-1 mediated p38 signaling pathway may be involved in the regulation of CIEO and CID during nematode defense against P. aeruginosa infection. Furthermore, in vitro experimental results also revealed that CIEO and CID exhibit good antibacterial effects, which may be associated with their antioxidant properties.Conclusion: Our results indicated that low-dose CIEO and CID treatment could activate the p38 signaling pathway in C. elegans, thereby regulating antimicrobial peptides, and achieving antimicrobial effects. Meanwhile, high doses of CIEO and CID might directly participate in the internal antimicrobial processes of C. elegans. Our study provides research basis for the antibacterial properties of CIEO and CID both in vivo and in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of cinnamon essential oil and its main component of cinnamaldehyde and the underlying mechanism

Shu,

Ge,

et al. 2024

Front. Pharmacol.

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“…Recently, it was found that paeoniflorin treatment could attenuate polystyrene nanoparticleinduced damage on reproductive capacity and germline development (Hua et al, 2023a). In addition, paeoniflorin treatment was helpful for nematodes against Pseudomonas aeruginosa infection and biofilm formation (Wang et al, 2023a).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it was found that paeoniflorin treatment could attenuate polystyrene nanoparticle-induced damage on reproductive capacity and germline development ( Hua et al, 2023a ). In addition, paeoniflorin treatment was helpful for nematodes against Pseudomonas aeruginosa infection and biofilm formation ( Wang et al, 2023a ). In this study, we further observed that treatment with 16–64 mg/L paeoniflorin could inhibit the glucose toxicity in reducing lifespan in nematodes ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin mitigates high glucose-induced lifespan reduction by inhibiting insulin signaling in Caenorhabditis elegans

2023

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In organisms, high glucose can cause several aspects of toxicity, including the lifespan reduction. Paeoniflorin is the major component of Paeoniaceae plants. Nevertheless, the possible effect of paeoniflorin to suppress high glucose toxicity in reducing lifespan and underlying mechanism are largely unclear. Thus, in this study, we examined the possible effect of paeoniflorin in suppressing high glucose (50 mM)-induced lifespan reduction and the underlying mechanism in Caenorhabditis elegans. Administration with 16–64 mg/L paeoniflorin could prolong the lifespan in glucose treated nematodes. Accompanied with this beneficial effect, in glucose treated nematodes, expressions of daf-2 encoding insulin receptor and its downstream kinase genes (age-1, akt-1, and akt-2) were decreased and expression of daf-16 encoding FOXO transcriptional factor was increased by 16–64 mg/L paeoniflorin administration. Meanwhile, the effect of paeoniflorin in extending lifespan in glucose treated nematodes was enhanced by RNAi of daf-2, age-1, akt-1, and akt-2 and inhibited by RNAi of daf-16. In glucose treated nematodes followed by paeoniflorin administration, the increased lifespan caused by daf-2 RNAi could be suppressed by RNAi of daf-16, suggesting that DAF-2 acted upstream of DAF-16 to regulate pharmacological effect of paeoniflorin. Moreover, in glucose treated nematodes followed by paeoniflorin administration, expression of sod-3 encoding mitochondrial Mn-SOD was inhibited by daf-16 RNAi, and the effect of paeoniflorin in extending lifespan in glucose treated nematodes could be suppressed by sod-3 RNAi. Molecular docking analysis indicated the binding potential of paeoniflorin with DAF-2, AGE-1, AKT-1, and AKT-2. Therefore, our results demonstrated the beneficial effect of paeoniflorin administration in inhibiting glucose-induced lifespan reduction by suppressing signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16-SOD-3 in insulin signaling pathway.

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“…Locomotion behavior was assayed by head thrash and body bend [ 56 ]. A head thrash is defined as one swing of the nematode body, and a body bend refers to the crawling of one wavelength [ 57 ].…”

Section: Methodsmentioning

confidence: 99%

Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Wang

2023

Toxics

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Recently, the transgenerational toxicity of nanoplastics has received increasing attention. Caenorhabditis elegans is a useful model to assess the transgenerational toxicity of different pollutants. In nematodes, the possibility of early-life exposure to sulfonate-modified polystyrene nanoparticle (PS-S NP) causing transgenerational toxicity and its underlying mechanisms were investigated. After exposure at the L1-larval stage, transgenerational inhibition in both locomotion behavior (body bend and head thrash) and reproductive capacity (number of offspring and fertilized egg number in uterus) was induced by 1–100 μg/L PS-S NP. Meanwhile, after exposure to 1–100 μg/L PS-S NP, the expression of germline lag-2 encoding Notch ligand was increased not only at the parental generation (P0-G) but also in the offspring, and the transgenerational toxicity was inhibited by the germline RNA interference (RNAi) of lag-2. During the transgenerational toxicity formation, the parental LAG-2 activated the corresponding Notch receptor GLP-1 in the offspring, and transgenerational toxicity was also suppressed by glp-1 RNAi. GLP-1 functioned in the germline and the neurons to mediate the PS-S NP toxicity. In PS-S NP-exposed nematodes, germline GLP-1 activated the insulin peptides of INS-39, INS-3, and DAF-28, and neuronal GLP-1 inhibited the DAF-7, DBL-1, and GLB-10. Therefore, the exposure risk in inducing transgenerational toxicity through PS-S NP was suggested, and this transgenerational toxicity was mediated by the activation of germline Notch signal in organisms.

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Treatment with paeoniflorin increases lifespan of Pseudomonas aeruginosa infected Caenorhabditis elegans by inhibiting bacterial accumulation in intestinal lumen and biofilm formation

Cited by 9 publications

References 90 publications

Antibacterial activity of cinnamon essential oil and its main component of cinnamaldehyde and the underlying mechanism

Antibacterial activity of cinnamon essential oil and its main component of cinnamaldehyde and the underlying mechanism

Paeoniflorin mitigates high glucose-induced lifespan reduction by inhibiting insulin signaling in Caenorhabditis elegans

Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

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