Treatment with compressed liquefied petroleum gas and ultrasound to improve cellulase activity

Silva, Juliana R.F.; Cantelli, Keli C.; Tres, Marcus V.; Rosa, Clarissa Dalla; Meirelles, M. Angela A.; Soares, Mónica Beatriz Alvarado; Oliveira, Débora de; Oliveira, J. Vladimir; Treichel, Helen; Mazutti, Márcio A.

doi:10.1016/j.bcab.2013.01.003

Cited by 14 publications

(10 citation statements)

References 16 publications

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“…System pressure also significantly affected the two processes. Nonetheless, its effect was more pronounced for US assisted-LPG hydrolysis, what is in good agreement with the study of Silva et al [17], where the effect of pressure on cellulase activity treated with US assisted-LPG was approximately 4 times higher than the effect for enzyme treated with pressurized LPG.…”

Section: Resultssupporting

confidence: 92%

“…This result can be attributed to partial deactivation of the enzyme when submitted to US-assisted LPG for long reaction time in a manner that enzyme load did not influence the hydrolysis in the studied range. On the other hand, it was demonstrated that pressure influences positively the enzyme activity for short treatment using US-assisted LPG [17]. However, it is expected that this increase in the activity occurs only for short period [11].…”

Section: Resultsmentioning

confidence: 99%

“…The range for enzyme load was based on preliminary tests (data not shown). The pressure was investigated in a range that LPG was in liquid phase and also because its effects on enzyme activity [17], whereas moisture content was chosen in a range that the bagasse is obtained in an industrial processing unit, avoiding the needs for additional unit operation before the hydrolysis.…”

Section: Experimental Apparatus and Proceduresmentioning

confidence: 99%

“…In a previous work, our research group evaluated the activity of a cellulase after treatment with pressurized liquefied petroleum gas (LPG) and US assisted-LPG [17]. After the combined treatment a 350% gain was observed in the enzyme activity.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

et al. 2015

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a b s t r a c tIn this work the enzymatic hydrolysis of sugarcane bagasse using pressurized liquefied petroleum gas (LPG) and ultrasound assisted pressurized LPG (US assisted-LPG) to obtain fermentable sugar in a unique step was investigated. For this purpose, non-treated sugarcane bagasse was used for enzymatic hydrolysis in a fixed-bed bioreactor. Several experiments were carried out to investigate the influence of moisture content, enzyme load and pressure on the yield of fermentable sugar after the hydrolysis. Maximum yield of fermentable sugar for hydrolysis carried out using pressurized LPG and US assisted-LPG was 245 and 217 g kg À1 , respectively. The main contribution of this work was to demonstrate that the use of alternative technologies (US assisted-LPG) has the potential to increase the efficiency of enzymatic hydrolysis of lignocellulosic materials without the needs of hard pre-treatment of the material and high enzyme loading.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Experimental Apparatus and Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

et al. 2015

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“…Este comportamento pode ser atribuído a maior eficiência das ondas ultrassônicas para romper a parede celular e liberar enzimas intracelulares da biomassa fúngica (MASON, 2003). O emprego de ultrassom como uma ferramenta para extração de metabólitos de origem vegetal e compostos bioativos de material vegetal ou animal (CHEMAT; HUMA; KHAN, 2011) tem sido crescente, uma vez que propicia maior eficiência de recuperação sem a necessidade de aumento do volume de extrator, o que gera menos danos ambientais e ao analista (KAPTUROWKA et al , 2012;SILVA et al, 2013).…”

Section: Resultsunclassified

Aplicação de carboxipeptidase obtida de Rhizopus na degradação de ocratoxina A

Kupski¹,

Alves²,

Garda‐Buffon³

et al. 2013

BBR - Biochem. Biotechnol.

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Mycotoxins are secondary metabolites produced by filamentous fungi in different food chain stages. Among mycotoxins, ochratoxin A (OTA) is of great importance, and presents carcinogenic, teratogenic and nephrotoxic effects. These effects have motivated the development of methods to reduce the contamination levels to the allowed levels. Biological methods involving the use of enzymes and microorganisms for OTA degradation has excelled due the specific reaction and mild conditions for detoxification. In this work we established conditions for the extraction of carboxypeptidase A from fermented biomass by Rhizopus oryzae in order to employ it in the OTA degradation, using pancreatin as control. The standardized methodology for enzymatic extraction consisted of ultrasonic agitation for 30 minutes in a fixed power of 150 W and 40 kHz. The enzyme extract of the microorganism showed a degradation capacity of 19.4%, which caused degradation higher (49%) than the one found during pancreatin action. This result confirms the advantage of using microbial enzymes over those from vegetables including micotoxycology biodegradation processes.Keywords: mycotoxin degradation, micro-organism, enzymatic hydrolysis.1 Universidade Federal do Rio Grande -Escola de Química e Alimentos. Caixa Postal 474 -CEP 96203900 Rio Grande -RS *Autor para correspondência larinekupski@yahoo.com.br RESUMOMicotoxinas são metabólitos secundários produzidos por fungos filamentosos em diferentes etapas da cadeia produtiva de alimentos. Entre micotoxinas, ocratoxina A (OTA) é de grande importância, e apresenta efeitos carcinogênicos, nefrotóxicos e teratogênicos. Estes efeitos vêm motivando o desenvolvimento de métodos capazes de diminuir esta contaminação aos níveis permitidos pela legislação. Os métodos biológicos envolvendo o uso de enzimas e microorganismos para degradação da OTA tem se destacado, em função da especificidade reacional e das condições brandas para a detoxificação. Neste trabalho foram estabelecidas condições de extração de carboxipeptidase A a partir de biomassa fermentada por Rhizopus oryzae para empregá-la na degradação de OTA, tendo pancreatina como controle. A metodologia padronizada para extração enzimática consistiu em agitação ultrassônica durante 30 minutos numa potencia fixa de 150 W e 40kHz. O extrato enzimático do microorganismo apresentou uma capacidade de degradação de 19,4%, que ocasionou uma degradação 49% superior a ação da pancreatina. Este resultado confirma a vantagem da utilização de enzimas microbianas em detrimento as de origem vegetal inclusive para processos de biodegradação micotoxicologica.Palavras-chave: degradação micotoxicológica; micro-organismo, hidrólise enzimática.

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Activity and stability of lipase from Candida Antarctica after treatment in pressurized fluids

et al. 2023

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Treatment with compressed liquefied petroleum gas and ultrasound to improve cellulase activity

Cited by 14 publications

References 16 publications

Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

Aplicação de carboxipeptidase obtida de Rhizopus na degradação de ocratoxina A

Activity and stability of lipase from Candida Antarctica after treatment in pressurized fluids

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