Treatment of wheat straw with alkaline hydrogen peroxide in a modified extruder

Gould, J.Michael; Jasberg, B. K.; Fahey, G. C.; Berger, L. L.

doi:10.1002/bit.260330215

Cited by 14 publications

(11 citation statements)

References 7 publications

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“…A wide range of pretreatments are known that differ in chemistry and mechanism but share the same outcome of increasing the accessibility of cell wall polysaccharides to cellulolytic enzymes [5]. Alkaline hydrogen peroxide (AHP) pretreatment is one such approach that has been studied since the 1980s [6-8]. AHP results in significant improvement in the enzymatic digestibility of commelinid monocots including corn stover and wheat straw [9,10] and can generate hydrolysates with more than 100 g/L of monomeric sugars that are fermentable without detoxification (manuscript in preparation).…”

Section: Introductionmentioning

confidence: 99%

Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings

Chen

Hegg

et al. 2013

Biotechnol Biofuels

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BackgroundOne route for producing cellulosic biofuels is by the fermentation of lignocellulose-derived sugars generated from a pretreatment that can be effectively coupled with an enzymatic hydrolysis of the plant cell wall. While woody biomass exhibits a number of positive agronomic and logistical attributes, these feedstocks are significantly more recalcitrant to chemical pretreatments than herbaceous feedstocks, requiring higher chemical and energy inputs to achieve high sugar yields from enzymatic hydrolysis. We previously discovered that alkaline hydrogen peroxide (AHP) pretreatment catalyzed by copper(II) 2,2΄-bipyridine complexes significantly improves subsequent enzymatic glucose and xylose release from hybrid poplar heartwood and sapwood relative to uncatalyzed AHP pretreatment at modest reaction conditions (room temperature and atmospheric pressure). In the present work, the reaction conditions for this catalyzed AHP pretreatment were investigated in more detail with the aim of better characterizing the relationship between pretreatment conditions and subsequent enzymatic sugar release.ResultsWe found that for a wide range of pretreatment conditions, the catalyzed pretreatment resulted in significantly higher glucose and xylose enzymatic hydrolysis yields (as high as 80% for both glucose and xylose) relative to uncatalyzed pretreatment (up to 40% for glucose and 50% for xylose). We identified that the extent of improvement in glucan and xylan yield using this catalyzed pretreatment approach was a function of pretreatment conditions that included H2O2 loading on biomass, catalyst concentration, solids concentration, and pretreatment duration. Based on these results, several important improvements in pretreatment and hydrolysis conditions were identified that may have a positive economic impact for a process employing a catalyzed oxidative pretreatment. These improvements include identifying that: (1) substantially lower H2O2 loadings can be used that may result in up to a 50-65% decrease in H2O2 application (from 100 mg H2O2/g biomass to 35–50 mg/g) with only minor losses in glucose and xylose yield, (2) a 60% decrease in the catalyst concentration from 5.0 mM to 2.0 mM (corresponding to a catalyst loading of 25 μmol/g biomass to 10 μmol/g biomass) can be achieved without a subsequent loss in glucose yield, (3) an order of magnitude improvement in the time required for pretreatment (minutes versus hours or days) can be realized using the catalyzed pretreatment approach, and (4) enzyme dosage can be reduced to less than 30 mg protein/g glucan and potentially further with only minor losses in glucose and xylose yields. In addition, we established that the reaction rate is improved in both catalyzed and uncatalyzed AHP pretreatment by increased solids concentrations.ConclusionsThis work explored the relationship between reaction conditions impacting a catalyzed oxidative pretreatment of woody biomass and identified that significant decreases in the H2O2, catalyst, and enzyme loading on the biomass as well a...

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Section: Introductionmentioning

confidence: 99%

Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings

Chen

Hegg

et al. 2013

Biotechnol Biofuels

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“…Therefore, in all of the experiments reported here, WS was introduced by pouring it directly into the barrel opening. A wooden ram was used to facilitate rapid transfer of the straw through the barrel opening as described by Gould et al (1989).…”

Section: Methodsmentioning

confidence: 99%

“…NaOH (Carr and Doane 1984), NaOH ] anthraquinone (Carr and Doane 1984), alkaline hydrogen peroxide (Gould et al 1989 ;Helming et al 1989), have all been reported to improve the nutritive quality of wheat straw, though the methods to assess this improvement have varied from in situ and in vivo to chemical analysis.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Extrusion Conditions on the Fermentability of Wheat Straw and Corn Silage

Williams

Poel

Bôer

et al. 1997

J. Sci. Food Agric.

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“…Alkaline peroxide oxidation pretreatment is one method that has been discovered to improve the accessibility of lignocellulosic biomass to cellulolytic enzymes (Kumar et al, 2009). This pretreatment approach has been studied extensively (Ayeni et al, 2013b;Wei and Cheng, 1985;Gould, 1984;Gould et al, 1989;Qi et al, 2009;Li et al, 2013). Studies on cellulosic biomass delignification (lignin removal) usually involve two different approaches, assuming that the lignocellulosic material contains one type of lignin, and that the material is composed of several species (usually initial, bulk, and residual lignin) dissolving at different rates (Kleinert, 1975;Springer, 1963).…”

Section: Introductionmentioning

confidence: 99%

EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae

Ayeni

Omoleye

Hymore

et al. 2016

Braz. J. Chem. Eng.

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-Shea tree sawdust delignification kinetic data during alkaline peroxide pretreatment were investigated at temperatures of 120 °C, 135 °C, and 150 °C. The activation energy during delignification was 76.4 kJ/mol and the Arrhenius constant was calculated as 8.4 x 10 6 per min. The reducing sugar yield for the treated to the untreated biomass was about 22-fold. Enzymatic hydrolysis conditions studied were; time (72 h and 96 h), substrate concentration (20, 30, 40, and 50 g/L), and enzyme loadings (10, 25, 40, 50 FPU/g dry biomass), which showed the optimum conditions of 96 h, 40 g/L, and 25 FPU/g dry biomass at 45 °C hydrolysis temperature. At the optimized enzymatic hydrolysis conditions, the reducing sugar yield was 416.32 mg equivalent glucose/g treated dry biomass. After 96 h fermentation of treated biomass, the ethanol obtained at 2% effective cellulose loading was 12.73 g/L. Alkaline peroxide oxidation pretreatment and subsequent enzymatic hydrolysis improved the ethanol yield of the biomass.

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Treatment of wheat straw with alkaline hydrogen peroxide in a modified extruder

Cited by 14 publications

References 7 publications

Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings

Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings

The Effect of Extrusion Conditions on the Fermentability of Wheat Straw and Corn Silage

EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae

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