EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae

Ayeni, Augustine O.; Omoleye, James; Hymore, F. K.; Pandey, R.A.

doi:10.1590/0104-6632.20160331s20140258

Cited by 14 publications

(5 citation statements)

References 41 publications

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“…Increasing the amount of celluclast and hydrolysis time will be proportional to the increase in the amount of glucose produced. Ayeni et al [20], reported the highest reducing sugar on shea tree sawdust in 4 day hydrolysis time, 50 FPU/g dry biomass, 45 o C, pH 4.8, 40 g/L substrate concentration was 482.40 mg/g dry biomass. In variations of the use of enzymes cellucase SHF process will affect the content of reducing sugar produced, can be seen in graph A, where the variation of cellulase enzymes 20 FPU/g produces (17.423 g/L for 72 h), more reduced sugar content than 10 FPU/g variation (11.423 g/L for 72 h), while the non treatment produced 1.718 g/L for 72 h. To effect substrate amount of raw materials also affect the content of reducing sugar obtained when the hydrolysis process is carried out on raw materials.…”

Section: Correlation Between Reducing Sugar and Ethanol In Shf And Ssmentioning

confidence: 96%

“…The variation of 20 g substrate could produced 19.233 g/L than 10 g substrate was 17.423 g/L and non treatment was obtained 1.718 g/L for 72 h. In this stage of saccharification enzyme, modified cellulose into cellobiose and further into simple sugars such as glucose, saccharification process using enzymes celluclast, enzyme hydrolysis process takes place at pH 4.8 and the temperature of 45-50 o C [21]. From Ganguly et al [22], and Aveni et al [20], the addition of biomass loading and hydrolysis time influences increase in reducing sugar, the maximum substrate loading to produces highest reducing sugar is 7% for 27.78 mg/g while the 40 g/L substrate concentration could produce maximum sugar yield for 42.4%.…”

Section: Correlation Between Reducing Sugar and Ethanol In Shf And Ssmentioning

confidence: 99%

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Pretreatment of Starch-Free Sugar Palm Trunk (Arenga pinnata) to Enhance Saccharification in Bioethanol Production

et al. 2018

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Starch-Free Sugar Palm Trunk (Arenga pinnata) can be utilized to produce bioethanol because of their high lignocellulosic contents. Production of bioethanol from lignocellulosic materials consist of pre-treatment, saccharification and fermentation processes. In this work, conversion of starch-free sugar palm trunk (Arenga pinnata) to fermentable sugar and bioethanol was carried out through g pretreatment, saccharification and fermentation processes. The pretreatment was carried out by addition of 1% (v/v) HNO3 and NH4OH for 30 min and 60 min, respectively. The saccharification was carried out at enzyme celullase loadings of 10 and 20 FPU/g and substrate loadings of 10 and 20 g for NH4OH pretreated samples. Fermentation was carried out using two methods i.e. separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) techniques. The results showed that pretreatment using NH4OH was more effective than HNO3 for 60 minutes. IFurthermore, the results also presented the reduction of the lignin content of 9.44% and the increase of cellulose content to 18.56% for 1% (v/v) NH4OH 60 min of pretreatment. The increase of enzyme cellulase (20 FPU/g substrate) and substrate loading (20 g) could produce more reducing sugar (17.423 g/L and 19.233 g/L) than that at 10 FPU/g substrate and 10 g substrate (11.423 g/L and 17.423 g/L), respectively. The comparison of SHF and SSF showed that SHF process yielded higher ethanol (8.11 g/L) as compared to SSF (3.95 g/L) and nontreatment process (0.507 g/L) for 72 h..

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Section: Correlation Between Reducing Sugar and Ethanol In Shf And Ssmentioning

confidence: 96%

Section: Correlation Between Reducing Sugar and Ethanol In Shf And Ssmentioning

confidence: 99%

Pretreatment of Starch-Free Sugar Palm Trunk (Arenga pinnata) to Enhance Saccharification in Bioethanol Production

et al. 2018

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“…The activation energy, in this case, can be defined as the minimum energy that is required by the molecules of pre-treatment solution to initiate delignification. 𝐾 𝐿 can be related to temperature following the Arrhenius law [2]:…”

Section: Delignification Parameters and Kineticsmentioning

confidence: 99%

“…Upstream delignification of biomass is the most crucial and rate limiting step of the whole process of depolymerisation of sugar polymers for obtaining biofuels [2]. There are a number of pre-treatment methods available to delignify biomasses; from the perspective of economy and environment friendliness alkaline peroxide based pre-treatment can be considered the most promising chemical method to delignify a biomass effectively without producing much inhibitors for the following steps of enzymatic hydrolysis [3,4].…”

Section: Introductionmentioning

confidence: 99%

Studies on delignification and inhibitory enzyme kinetics of alkaline peroxide pre-treated pine and deodar saw dust

Baksi

Sarkar

Saha

et al. 2019

Chemical Engineering and Processing - Process Intensification

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Delignification of lignocellulosic biomass by alkaline peroxide pre-treatment is a preliminary important step for an overall biomass fractionation process. In the present work, saw dusts are pre-treated by aqueous alkaline peroxide solution under different temperatures over a predetermined time. It is seen that Combined Pre-treatment (CP) removes a substantially higher quantity of lignin from biomass under a particular temperature. At elevated temperatures, the extent of delignification is observed much better. The % removal is: [PR:19.35%(30°C): 25.26%(50°C): 33.30%(100 °C)]; [CD: 14.64%(30°C): 23.64%(50°C):28.83%(100 °C)]. Batch kinetics is investigated with certain models and corresponding parameters are estimated. As pre-treatment severity is strongly correlated to the pre-treatment temperature, increased value of "potential degree of delignification" is observed at escalated temperatures. Kinetics of enzymatic hydrolysis of delignified biomass shows decreased product inhibition with increased substrate concentration under a particular enzyme loading.Starting with a combination of 50 g/L substrate concentration with an enzyme loading of 13.23 g/L, an optimum concentration of 17.2 g/L and 21.19 g/L of glucose are produced from Pinus roxburghii and Cedrus deodara respectively. Experimental data fit quite well with the competitive inhibition kinetics based theoretical models with r 2 ≥0.95. It is inferred that enzymes are competitively inhibited by glucose.

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“…Based on literature, the mechanisms for lignin removal from plant biomass bring about size reduction of the substrate, physical redistribution of the components, depolymerization and solubilization [11]. Shea-tree sawdust delignification kinetics has been investigated [12]. The raw material was pretreated at 120-150°C.…”

Section: Introductionmentioning

confidence: 99%

Chemical Kinetics of Alkaline Pretreatment of Napier Grass (Pennisetum purpureum) Prior Enzymatic Hydrolysis

Sanni¹,

Akinrinola²,

Yusuf³

et al. 2018

TOCENGJ

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Background:Napier grass is a naturally abundant waste material that can be cultivated over a vast area of land which makes it a viable source for sugar and bioethanol production.Introduction:The presence of lignin in the biomass makes cellulose inaccessible for conversion to useful products, however, in order to provide for efficient utilization of the waste material, reagent and energy, a study on the kinetics of lignin removal from Napier grass was carried out in this work using 1 and 3 w/w % NaOH at temperatures between 80 and 120°C.Materials & Methods:Based on the investigation, there was increased lignin removal for increased NaOH concentration. Kinetic parameters were also determined and it was observed that, the reaction of lignin in Napier grass with NaOH obeys a pseudo-zero or pseudo-fractional order kinetics. Furthermore, the orders of the reaction for the pretreatment conditions of 3 w/w% NaOH at 100°C and those of 3 and 1 w/w NaOH at 120°C gave close reaction orders of 0.2, 0.22 and 0.24 respectively after 110 minutes, which implies that, for the three cases, the residual lignin in the extract was almost the same at the pretreatment conditions while slight differences are evident in their pseudo rate constants. Also, it was observed that, the activation energy of the reaction reduced significantly as the concentration of NaOH increased from 1w/w - 3 w/w%.Conclusion:Based on the AIL and the total lignin (i.e.AIL + ASL) in the Napier grass, the recorded delignification efficiencies at the optimum pretreatment time of 17.5 h are 90 and 76% respectively. In addition, the adopted Differential Technique (DT) combined with the Ostwald Method of Isolation (OMI) can be accurately used to study the kinetics of lignin removal from Napier grass.

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EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae

Cited by 14 publications

References 41 publications

Pretreatment of Starch-Free Sugar Palm Trunk (Arenga pinnata) to Enhance Saccharification in Bioethanol Production

Pretreatment of Starch-Free Sugar Palm Trunk (Arenga pinnata) to Enhance Saccharification in Bioethanol Production

Studies on delignification and inhibitory enzyme kinetics of alkaline peroxide pre-treated pine and deodar saw dust

Chemical Kinetics of Alkaline Pretreatment of Napier Grass (Pennisetum purpureum) Prior Enzymatic Hydrolysis

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