Treatment of vitiligo in children and adolescents by autologous cultured pure melanocytes transplantation with comparison of efficacy to results in adults

Hong, Weisong; Hu, Dan‐Ning; Qian, Guoqing; McCormick, Susan; Xu, Ai-e

doi:10.1111/j.1468-3083.2010.03824.x

Cited by 29 publications

(27 citation statements)

References 27 publications

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“…Clinically, transplantation of autologous melanocytes therapy is proven to be the most effective therapy option for the vitiligo patients [14], [15], [16]. Groups including ours have treated vitiligo patients using this method with the cure rate above 53% [14], [15].…”

Section: Introductionmentioning

confidence: 93%

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Dermal Mesenchymal Stem Cells (DMSCs) Inhibit Skin-Homing CD8+ T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation Efficiency

Zhou

Zhang

et al. 2013

PLoS ONE

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We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells (DMSCs) on CD8+ T cell activities in vitro. Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients’ autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.

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Section: Introductionmentioning

confidence: 93%

“…Groups including ours have treated vitiligo patients using this method with the cure rate above 53% [14], [15]. Studies showed that the number of cytotoxic CD8+ T cells was higher in active disease than in stable disease [17].…”

Section: Introductionmentioning

confidence: 97%

Dermal Mesenchymal Stem Cells (DMSCs) Inhibit Skin-Homing CD8+ T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation Efficiency

Zhou

Zhang

et al. 2013

PLoS ONE

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“…Melanocytes were isolated and cultured by methods based on those described previously (16,17). Primary melanocytes (passage 3–5) from vitiligo patients or normal controls were seeded into 6-well plates at a density of 1×10 5 cells/well and kept in an incubator for 4 h to allow them to attach.…”

Section: Methodsmentioning

confidence: 99%

CD8+ T cells from vitiligo perilesional margins induce autologous melanocyte apoptosis

Wu¹,

Zhou²,

Wan³

et al. 2012

Molecular Medicine Reports

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Cell-mediated autoimmunity has been suggested to be involved in the melanocyte apoptosis that occurs in vitiligo. We investigated the cytotoxicity to autologous melanocytes of CD8+ T cells from the perilesional margins and peripheral blood samples of vitiligo patients. CD8+ T cells isolated from skin biopsied from the edges of depigmented skin patches of vitiligo patients or from peripheral blood samples of the same donors were proliferated in culture medium. The primary cultures of CD8+ T cells and autologous melanocytes were mixed at ratios of 1:1, 1:2 or 1:5 and incubated for 3 days. The apoptosis of the melanocytes was analyzed by flow cytometry. Secreted cytokines in selected samples were measured by cytokine arrays. The results show that the CD8+ T cells were successfully isolated from the vitiligo perilesional margins. This cell population showed a significantly higher percentage of CD69 expression (56.13±3.55 versus 29.93±2.35%, p<0.01) and CD137 expression (41.74±1.06 versus 25.97±1.63%, p<0.01) compared with CD8+ T cells in peripheral blood from the same donors. The co-culturing of CD8+ T cells from lesional skin with autologous melanocytes induced apoptosis in the melanocytes (16.63±1.21, 16.71±0.63 and 18.32±1.60% for CD8+ T cells and autologous melanocytes at ratios of 1:1, 1:2 and 1:5, respectively). IL-6 levels were much higher in the co-culture (3.01-fold higher than in a melanocyte monoculture and 17.32-fold higher than in a CD8+ T-cell monoculture). The CD8+ T cells were also demonstrated to secrete more IL-13. Taken together, our data demonstrate that the infiltration of active CD8+ T cells takes place in the vitiligo perilesional margins. Those CD8+ T cells present significantly higher activation levels and higher cytotoxicity to autologous melanocytes than their counterparts from peripheral blood samples. These data suggest that CD8+ T cells are likely to be involved in the pathogenesis of vitiligo.

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“…A number of dermatosurgery techniques are available to promote repigmentation of vitiligo in adults and children, such as mini- or punch grafts, split-thickness skin grafts, cultured epidermal sheets, cultured melanocyte suspensions, follicular grafts and suction blister grafts. [7–18] Among these methods, the highest success rates have been achieved with split-thickness skin grafts and epidermal blister grafts. For better results, phototherapy or photochemotherapy of donor sites can also be performed after or before grafting.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of suction blister epidermal graft without phototherapy for locally stable and resistant vitiligo

2012

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Introduction:Surgical methods for treatment of vitiligo include punch grafts, blister grafts, follicular grafts and cultured melanocyte grafts. The aim of this study was to determine the efficacy of suction blister grafts for treatment of vitiligo, without the use of phototherapy.Materials and Methods:This clinical trial study was conducted on 10 patients with vitiligo that was resistant to usual treatments and with limited involvement in the affected sites. We used cryotherapy and a manual suction device for blistering at the recipient and donor sites, respectively. The blister was separated and fixed with sutures and a dressing to the recipient site. Repigmentation of lesions was evaluated monthly for 6 months after treatment. Repigmentation rates higher than 90%, between 71%–90%, from 51%–70%, and less than 50% were graded as complete, good, moderate, and poor, respectively.Results:Ten patients (five females with a mean age of 23.2±3.96 years and five males with a mean age of 30.60±4.15 years) were enrolled in the study. Reponses to treatment after a 6-month follow-up were ‘complete,’ ‘good,’ and ‘moderate’ in 7 (70%), 1 (10%), and 2 (20%) patients, respectively.Conclusion:With this technique, patients with restricted sites of involvement, that did not respond to the usual treatments showed very good repigmentation without any additional phototherapy over a 6-month follow-up; moreover, there were no side effects such as scarring.

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Treatment of vitiligo in children and adolescents by autologous cultured pure melanocytes transplantation with comparison of efficacy to results in adults

Cited by 29 publications

References 27 publications

Dermal Mesenchymal Stem Cells (DMSCs) Inhibit Skin-Homing CD8+ T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation Efficiency

Dermal Mesenchymal Stem Cells (DMSCs) Inhibit Skin-Homing CD8+ T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation Efficiency

CD8+ T cells from vitiligo perilesional margins induce autologous melanocyte apoptosis

Efficacy of suction blister epidermal graft without phototherapy for locally stable and resistant vitiligo

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