Treatment of gastric ulcers and diarrhea with the Amazonian herbal medicine sangre de grado

Miller, Mark J.S.; MacNaughton, Wallace K.; Zhang, Xiaojing; Thompson, Jane H.; Charbonnet, Randi M; Bobrowski, Paul; Lao, Juan; Trentacosti, Ann Marie; Sandoval, Manuel

doi:10.1152/ajpgi.2000.279.1.g192

Cited by 55 publications

(53 citation statements)

References 23 publications

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“…In addition to these well-known effects on sensory nerves, capsaicin has been used as a diarrhea-inducing reagent in in vitro systems (Moriarty et al, 2001). This latter effect of capsaicin may be independent of its action on nerve cells (Miller et al, 2000). The topology of VR1 shows a characteristic transmembrane domain that contains six putative ␣-helices.…”

Section: ϩmentioning

confidence: 98%

“…Although those complications are mainly associated with steatorrhea resulting from the deficiency of pancreatic enzyme secretion to digest fat (Rubinstein et al, 1986), the impaired fluid secretion caused by defects of Cl Ϫ transport in the intestinal epithelia also may play a pathophysiological role in gastrointestinal symptoms of CF patients (Gabriel et al, 1994;Binkovitz et al, 1999). On the other hand, capsaicin has been reported to cause diarrhea by increasing fluid secretion accompanied with stimulation of intestinal ion transport (Miller et al, 2000). It has been suggested that the mechanism for capsaicin-activated ion transport may involve stimulations of the sensory neurons to release neurotransmitters such as substance P and neurokinin (Vanner and MacNaughton, 1995;Moriarty et al, 2001).…”

Section: Capsaicin On Cftr Chloride Channel 1421mentioning

confidence: 99%

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Capsaicin Potentiates Wild-Type and Mutant Cystic Fibrosis Transmembrane Conductance Regulator Chloride-Channel Currents

Ai¹,

Bompadre²,

Wang³

et al. 2004

Mol Pharmacol

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To examine the effects of capsaicin on cystic fibrosis transmembrane conductance regulator (CFTR), we recorded wildtype and mutant CFTR chloride-channel currents using patchclamp methods. The effects of capsaicin were compared with those of genistein, a well-characterized CFTR activator. In whole-cell experiments, capsaicin potentiates cAMP-stimulated wild-type CFTR currents expressed in NIH 3T3 cells or Chinese hamster ovary cells in a dose-dependent manner with a maximal response ϳ60% of that with genistein and an apparent K d of 48.4 Ϯ 6.8 M. In cell-attached recordings, capsaicin alone fails to activate CFTR in cells that show negligible basal CFTR activity, indicating that capsaicin does not stimulate the cAMP cascade. The magnitude of potentiation with capsaicin depends on the channel activity before drug application; the lower the prestimulated P o , the higher the potentiation. Single-channel kinetic analysis shows that capsaicin potentiates CFTR by increasing the opening rate and decreasing the closing rate of the channel.

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Section: ϩmentioning

confidence: 98%

Section: Capsaicin On Cftr Chloride Channel 1421mentioning

confidence: 99%

Capsaicin Potentiates Wild-Type and Mutant Cystic Fibrosis Transmembrane Conductance Regulator Chloride-Channel Currents

Ai¹,

Bompadre²,

Wang³

et al. 2004

Mol Pharmacol

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“…1 Extracts (or infusions) of the different parts of the plant of several species of the genus Croton as C. lechleri, C. palanostigma, C. draconoides and C. urucurana are traditional remedies well known for their healing powers and are extensively used by indigenous cultures of the Amazon River for the treatment of infected wounds and to hasten wound healing. [2][3][4][5][6][7][8] Its stem bark, when slashed, releases a blood-red sap and, for this reason, C. urucurana and other species of the genus Croton are known as Dragon's Blood (or Sangra d'água in Brazil).…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and evaluation of antibacterial and antioxidant activities of the essential oil of Croton urucurana Baillon (Euphorbiaceae) stem bark

Simionatto¹,

Bonani²,

Morel³

et al. 2007

J. Braz. Chem. Soc.

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O óleo essencial obtido a partir das cascas do caule de Croton urucurana Baillon (Euphorbiaceae) foi analisado por CG e CG-EM. 83 compostos foram identificados, sendo borneol (14,7%), acetato de bornila (5,2%), 1-isopropil-7-metil-4-metileno-1,3,4,5,6,8-hexaidro-2H-naftalen-4a-ol (14,7%), sesquicineol (10,5%) e epóxido de γ-gurjuneno (5,4%) os principais componentes. Para o óleo essencial bruto, foi determinada em 3,21 mg mL -1 a EC 50 no ensaio de atividade antioxidante frente ao radical livre DPPH. A fração do óleo essencial bruto que apresentou atividade antioxidante foi purificada por CCD sobre sílica gel. Análises de CG e CG-EM revelaram que α-bisabolol (38,3%), α-eudesmol (9,3%) e guaiol (8,2%) são os principais componentes da fração antioxidante. A EC 50 medida no teste frente ao DPPH foi de 1,05 mg mL -1 para a fração bioativa. Foi determinada a atividade antimicrobiana do óleo essencial bruto frente a sete bactérias Gram-positivas e Gram-negativas e três fungos. Os valores de CIM medidos variaram de 1,25 a 10,00 mg mL -1 .The essential oil obtained from the stem bark of Croton urucurana Baillon (Euphorbiaceae) was analysed by GC and GC-MS. 83 compounds were identified and borneol (14.7%), bornyl acetate (5.2%), 1-isopropyl-7-methyl-4-methylene-1,3,4,5,6,8-hexahydro-2H-naphthalen-4a-ol (14.7%), sesquicineole (10.5%) and γ-gurjunene epoxide (5.4%) were the main components. The EC 50 value of the crude essential oil in the DPPH free radical scavenging assay was 3.21 mg mL -1 . The fraction of the crude essential oil that presented antioxidant activity was purified by prep-TLC on silica gel. GC and GC-MS analysis revealed that α-bisabolol (38.3%), α-eudesmol (9.3%) and guaiol (8.2%) were the main components of the antioxidant fraction. The EC 50 value measured for the bioactive oil fraction in the DPPH assay was 1.05 mg mL -1 . The antimicrobial activity of the crude essential oil was assayed against seven Gram-positive and Gram-negative bacteria and three yeasts. Measured MIC values ranged from 1.25 to 10.00 mg mL -1 . Keywords:Croton urucurana, essential oil, antioxidant, DPPH assay, antimicrobial acticity IntroductionCroton (Euphorbiaceae) is one of the largest genera of flowering plants, with nearly 1,300 species of herbs, shrubs, and trees that are ecologically prominent and often important elements of secondary vegetation in the tropics and subtropics worldwide. Previous works reported that the essential oils from northeastern Brazilian Croton species, C. zenhtneri, C. nepetaefolius and C. argyrophylloides, exhibited good antioxidant activities. 9 Croton urucurana Baillon (Euphorbiaceae) is a tree commonly found in Paraguay, northern Argentina, southern Brazil, and Uruguay. Three different products from this species are used primarily in folk medicine: the red sap, the stem bark, and the gum exudate. 10 Many pharmacological effects of Croton urucurana have been described in literature, which include wound and ulcer healing, antidiarrhoeic, anticancer, intestinal anti-inflammatory, antioxidant and an...

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“…Existen varios estudios fitoquímicos (7,8) y farmacológicos realizados en el extranjero y nacionales, en los que nuestro equipo ha contribuido con investigaciones que reconocen su valor protector en la mucosa gástrica de ratas, tanto de la resina como de su fracción alcaloidea denominada taspina (9)(10)(11) ; asimismo, se ha profundizado en cierto grado sobre su probable mecanismo de acción (12)(13)(14) . También, se ha realizado evaluaciones preclínicas que han validado sus propiedades cicatrizantes en la piel de animales de experimentación (3,15,16) y se ha estudiado su potencial toxicológico (11,(17)(18)(19)(20)(21) .…”

Section: Introductionunclassified

“…También, se ha realizado evaluaciones preclínicas que han validado sus propiedades cicatrizantes en la piel de animales de experimentación (3,15,16) y se ha estudiado su potencial toxicológico (11,(17)(18)(19)(20)(21) .…”

Section: Introductionunclassified

Evaluación de la toxicidad vaginal de Croton lechleri en conejas

et al. 2011

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Resumen Objetivo: Evaluar la toxicidad vaginal de una preparación en gel de Croton lechleri en conejas. Diseño: Estudio experimental. Institución: Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón, Instituto de Investigaciones Clínicas, Instituto de Patología de la Facultad de Medicina, y Facultad de Farmacia, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Resina de Croton lechleri y conejos hembras, blancos, Nueva Zelanda. Métodos: Los animales fueron distribuidos en tres grupos (n=5): I. Control, vehículo; II, C. lechleri 1%; y III, C. lechleri 5%; los conejos recibieron estas muestras en un volumen de 1 mL por vía intravaginal, una vez/día, durante 10 días consecutivos. Se observó el estado clínico general, así como la apariencia física de la vagina y el perineo por signos de flujo, eritema y edema. Al final del experimento se realizó la exéresis quirúrgica de la vagina de cada coneja y se evaluó macroscópicamente, buscando signos de irritación, sangrado o daño de la mucosa. Luego, se fijó en formol tamponado al 10% y posteriormente se tomó tres muestras representativas de las regiones cérvico-vaginal, media-vaginal, y urovaginal, para el estudio histopatológico. Principales medidas de resultados: Irritación vaginal. Resultados: El examen macroscópico de la vagina en el grupo control mostró aspecto congestivo leve en forma difusa, en todos los casos, y de aspecto similar en las muestras del grupo Croton lechleri al 1%. En la formulación al 5%, en dos casos se observó congestión petequial parcial leve. El estudio histopatológico reveló un puntaje promedio de irritación vaginal de 3,4 a 6 y 3,6 a 8 para las preparaciones al 1% y 5%, respectivamente. Conclusión: En nuestras condiciones experimentales, las preparaciones de gel de C. lechleri evaluadas fueron no irritantes para la mucosa vaginal y resultaron aceptables. Palabras clave: Toxicidad; vagina; conejos; patología. Croton lechleri resin and female white New Zealand rabbits. Methods: The animals were divided into three groups (n=5): I. Control, vehicle; II, C. lechleri 1%, and III, C. lechleri 5%; they received samples of 1 mL volume intravaginally 1 time/day for 10 consecutive days. The general clinical condition and physical appearance of the vagina and the perineum for signs of flow, erythema and edema were observed. At the end of the experiment surgical resection of the vagina of each rabbit was performed and signs of irritation, bleeding or mucosal damage were determined macroscopically. Specimens were placed in 10% buffered formalin and cervico-vaginal, mid-vaginal, and uro-vaginal samples underwent histopathology study. Main outcome measures: Vaginal irritation. Results: Gross examination of the vagina in both the control and the Croton lechleri 1% groups showed mild diffuse congestion. Two cases of Croton lechleri 5% formulation showed mild petechial congestion. Histopathology study revealed a vaginal irritation average score of respectively 3.4 to 6, and 3.6 to 8 for the 1% and 5% preparations. Conc...

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Treatment of gastric ulcers and diarrhea with the Amazonian herbal medicine sangre de grado

Cited by 55 publications

References 23 publications

Capsaicin Potentiates Wild-Type and Mutant Cystic Fibrosis Transmembrane Conductance Regulator Chloride-Channel Currents

Capsaicin Potentiates Wild-Type and Mutant Cystic Fibrosis Transmembrane Conductance Regulator Chloride-Channel Currents

Chemical composition and evaluation of antibacterial and antioxidant activities of the essential oil of Croton urucurana Baillon (Euphorbiaceae) stem bark

Evaluación de la toxicidad vaginal de Croton lechleri en conejas

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