Treatment of donor cells with trichostatin A improves in vitro development and reprogramming of buffalo (Bubalus bubalis) nucleus transfer embryos

Luo, Chen; Lu, Fenghua; Wang, Xiaoli; Wang, Zhiqiang; Li, Xiangping; Gong, Fangqiang; Jiang, Jianrong; Liu, Qingyou; Shi, Dongquan

doi:10.1016/j.theriogenology.2013.07.013

Cited by 31 publications

(17 citation statements)

References 38 publications

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“…Recently, Luo et al . (2013) found that treatment of donor cells with 150 or 300 μM TSA increased cleavage and blastocyst formation rates of swamp buffalo cloned embryos [29]. Treatment with 500–1000 nM scriptaid, another histone deacetylase inhibitor, has been shown to improve the developmental potential of handmade river buffalo cloned embryos [30].…”

Section: Discussionmentioning

confidence: 99%

Effects of Trichostatin A on In Vitro Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (Bubalus bubalis) Cloned Embryos

Srirattana

Ketudat-Cairns

Nagai

et al. 2014

Journal of Reproduction and Development

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Trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. This brings our attention to investigation of the effects of TSA on developmental potential of swamp buffalo cloned embryos. Swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes. After fusion, reconstructed oocytes were treated with 0, 25 or 50 nM TSA for 10 h. The results showed that there was no significant difference in the rates of fusion (82–85%), cleavage (79–84%) and development to the 8-cell stage (59–65%) among treatment groups. The highest developmental rates to the morula and blastocyst stages of embryos were found in the 25 nM TSA-treated group (42.7 and 30.1%, respectively). We also analyzed the DNA methylation level in the satellite I region of donor cells and in in vitro fertilized (IVF) and cloned embryos using the bisulfite DNA sequencing method. The results indicated that the DNA methylation levels in cloned embryos were significantly higher than those of IVF embryos but approximately similar to those of donor cells. Moreover, there was no significant difference in the methylation level among TSA-treated and untreated cloned embryos. Thus, TSA treatments at 25 nM for 10 h could enhance the in vitro developmental potential of swamp buffalo cloned embryos, but no beneficial effect on the DNA methylation level was observed.

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Section: Discussionmentioning

confidence: 99%

Effects of Trichostatin A on In Vitro Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (Bubalus bubalis) Cloned Embryos

Srirattana

Ketudat-Cairns

Nagai

et al. 2014

Journal of Reproduction and Development

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“…Moreover, histone hyperacetylation in donor cells and/or SCNT embryos could improve their further developmental competence (Enright, Kubota, Yang, & Tian, ; Li et al., ; Wang, Xiong, et al., ). Currently, research reports showed that the level of histone acetylation and cloning development can be increased by histone deacetylase inhibitors (HDACis) such as trichostatin A (TSA) (Iager et al., ; Kishigami et al., ; Luo et al., ; Yamanaka, Sugimura, Wakai, Kawahara, & Sato, ), scriptaid (Van Thuan et al., ; Wang, Zhang, et al., ; Zhao et al., ) and valproic acid (VPA) (Costa‐Borges, Gonzalez, Santalo, & Ibanez, ; Miyoshi et al., ; Xu et al., ). These HDACis such as TSA and scriptaid, with their hydroxamic acid group and five‐carbon atom linker to the phenyl group, had the optimal conformation to fit into the active site which specifically binds zinc‐dependent hydrolases of histone deacetylases (HDACs) (Finnin et al., ).…”

Section: Introductionmentioning

confidence: 99%

Suberoylanilide hydroxamic acid, a novel histone deacetylase inhibitor, improves the development and acetylation level of miniature porcine handmade cloning embryos

Sun

Cui

et al. 2017

Reprod Domestic Animals

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Histone deacetylase inhibitors (HDACis) can change the histone acetylation and significantly enhance the developmental competence of the pre-implantation SCNT embryo. To select a proper histone deacetylase inhibitor to improve the success rate and potentially developmental ability of handmade cloning (HMC) embryos of miniature porcine, we compared the effect of two histone deacetylase inhibitors (SAHA vs. VPA) on HMC embryo development, their histone acetylation level and the expression level of relevant genes. The blastocyst rate and number of blastocyst cells of HMC embryos treated with SAHA (SAHA-HMC) or VPA (VPA-HMC) were significantly higher than those of control (Control-HMC), respectively, but there were no significant difference between SAHA-HMC and VPA-HMC groups. In addition, the acetylation level (AcH4K8) of Control-HMC and VPA-HMC embryos at the blastocyst stage, respectively, was significantly lower than that of in vitro fertilized (IVF) and SAHA-HMC embryos. However, the acetylation H4K8 of the blastocysts had no significant difference between SAHA-HMC and the IVF groups. The SAHA-HMC blastocysts indicated comparative expression levels of Oct4 and HDAC1 (histone deacetyltransferase gene) with those of IVF blastocysts. In contrast, the expression levels of Oct4 were lower and those of HDAC1 were higher in the VPA-HMC and Control-HMC blastocysts, respectively, compared to those of the IVF blastocysts. Our results demonstrated that the HMC embryos treated by SAHA could promote the pre-implantation development and increase the levels of histone H4K8 acetylation and the expression of the OCT4 gene, yet decrease the expression of the HDAC1 gene to the comparable level of the IVF embryos. Our results proved that SAHA may be a better histone deacetylase inhibitor for porcine HMC compared to VPA, and furthermore, it may indicate that SAHA can effectively correct the abnormal histone acetylation during the HMC embryo development and subsequently improve the full-term developmental potential of the HMC embryos after embryo transplantation.

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“…Aza-dC acts as an analogue of the cytosine base with the loss of methylation ability. However, the most efficient drugs for reprogramming donor cells for nuclear transfer are targeting histone acetylation, by way of HDAC (histone deacetylase) inhibitors (Biran and Meshorer, 2012; Gaspar-Maia et al, 2011) with trichostatin A (TSA) as the most widely tested molecule (Enright et al, 2003; Luo et al, 2013). Another reprogramming strategy in mammal is to use oocyte extracts, mainly from Xenopus (Liu et al, 2014).…”

Section: Somatic Cells: Strength and Pitfalls Of Preservation And mentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Treatment of donor cells with trichostatin A improves in vitro development and reprogramming of buffalo (Bubalus bubalis) nucleus transfer embryos

Cited by 31 publications

References 38 publications

Effects of Trichostatin A on <i>In Vitro</i> Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (<i>Bubalus bubalis</i>) Cloned Embryos

Effects of Trichostatin A on <i>In Vitro</i> Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (<i>Bubalus bubalis</i>) Cloned Embryos

Suberoylanilide hydroxamic acid, a novel histone deacetylase inhibitor, improves the development and acetylation level of miniature porcine handmade cloning embryos

Cryobanking of aquatic species

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