Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer

Mizutani, Eiji; Wakayama, Sayaka; Wakayama, Teruhiko

doi:10.1007/978-1-4939-1594-1_8

Cited by 8 publications

(7 citation statements)

References 41 publications

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“…Excess histone acetylation may account in part for increased rates of aneuploidy seen in cloned constructs made by transferring somatic cell nuclei to oocytes (Mizutani et al, 2012; Nolen et al, 2005). Although histone deacetylase inhibitors (HDACis) may help program some genes for expression in clones to improve early development (Laguna‐Barraza, Sanchez‐Calabuig, Gutierrez‐Adan, Rizos, & Perez‐Cerezales, 2018; Mizutani, Wakayama, & Wakayama, 2015), a potential downside could be disruption of spindle formation and possibly aneuploidy. Histone deacetylation may be sensitive to exogenous factors such as excessive follicle stimulating hormone or stress, which can also induce oocyte aneuploidy (Roberts et al, 2005; Salvador et al, 2001; Xu et al, 2011).…”

Section: Oocyte Histones and Histone Modifiersmentioning

confidence: 99%

Listening to mother: Long‐term maternal effects in mammalian development

Ruebel

Latham

2020

Molecular Reproduction Devel

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The oocyte is a complex cell that executes many crucial and unique functions at the start of each life. These functions are fulfilled by a unique collection of macromolecules and other factors, all of which collectively support meiosis, oocyte activation, and embryo development. This review focuses on the effects of oocyte components on developmental processes that occur after the initial stages of embryogenesis. These include long-term effects on genome function, metabolism, lineage allocation, postnatal progeny health, and even subsequent generations.Factors that regulate chromatin structure, genome programming, and mitochondrial function are elements that contribute to these oocyte functions. K E Y W O R D S chromatin, embryo gene regulation, maternal effect, oocyte 400 |

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Section: Oocyte Histones and Histone Modifiersmentioning

confidence: 99%

Listening to mother: Long‐term maternal effects in mammalian development

Ruebel

Latham

2020

Molecular Reproduction Devel

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“…This may be due to incomplete reprogramming of SCNT embryos, leading to the abnormality of cloned pigs [ 1 ]. Thus, methods to improve embryo quality would be applied in the further pig cloning [ 27 ]. Importantly, it is observed that surrogate ovulation status is associated with the overall cloning efficiency, so, the strategy to coordinate the synchronization between cloned embryos and surrogates in the further pig cloning, such as ovulation detection by ultrasonography, would be particularly important.…”

Section: Discussionmentioning

confidence: 99%

Ovulation Statuses of Surrogate Gilts Are Associated with the Efficiency of Excellent Pig Cloning

Xie

et al. 2015

PLoS ONE

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Somatic cell nuclear transfer (SCNT) is an assisted reproductive technique that can produce multiple copies of excellent livestock. However, low cloning efficiency limits the application of SCNT. In this study, we systematically investigated the major influencing factors related to the overall cloning efficiency in pigs. Here, 13620 cloned embryos derived from excellent pigs were transferred into 79 surrogate gilts, and 119 live cloned piglets were eventually generated. During cloning, group of cloned embryos derived from excellent Landrace or Large white pigs presented no significant differences of cleavage and blastocyst rates, blastocyst cell numbers, surrogate pregnancy and delivery rates, average numbers of piglets born and alive and cloning efficiencies, and group of 101–150, 151–200 or 201–250 cloned embryos transferred per surrogate also displayed a similar developmental efficiency. When estrus stage of surrogate gilts was compared, group of embryo transfer on Day 2 of estrus showed significantly higher pregnancy rate, delivery rate, average number of piglets born, average alive piglet number or cloning efficiency than group on Day 1, Day 3, Day 4 or Day 5, respectively (P<0.05). And, in comparison with the preovulation and postovulation groups, group of surrogate gilts during periovulation displayed a significantly higher overall cloning efficiency (P<0.05). Further investigation of surrogate estrus stage and ovulation status displayed that ovulation status was the real factor underlying estrus stage to determine the overall cloning efficiency. And more, follicle puncture for preovulation, not transfer position shallowed for preovulation or deepened for postovulation, significantly improved the average number of piglets alive and cloning efficiency (P<0.05). In conclusion, our results demonstrated that ovulation status of surrogate gilts was the fundamental factor determining the overall cloning efficiency of excellent pigs, and follicle puncture, not transfer position change, improved cloning efficiency. This work would have important implications in preserving and breeding excellent livestock and improving the overall cloning efficiency.

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“…The nuclear donor cell is a crucial factor affecting SCNT embryo quality and the efficiency of SCNT, and many efforts have been made to improve them by improving donor-cell status Mizutani et al, 2015). Furthermore, miR-101 can regulate cell proliferation, the cell cycle, and apoptosis in different cell types (Wang et al, 2014b;Zhou et al, 2015;Qian et al, 2016).…”

Section: Effects Of Mir-101-2 Overexpression On Apoptosis Cell Cyclementioning

confidence: 99%

Overexpression of miR-101-2 in donor cells improves the early development of Holstein cow somatic cell nuclear transfer embryos

Chang

Xie

Zhang

et al. 2019

Journal of Dairy Science

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Accumulating studies have suggested that microRNA play a part in regulating multiple cellular processes, such as cell proliferation, apoptosis, the cell cycle, and embryo development. This study explored the effects of miR-101-2 on donor cell physiological status and the development of Holstein cow somatic cell nuclear transfer (SCNT) embryos in vitro. Holstein cow bovine fetal fibroblasts (BFF) overexpressing miR-101-2 were used as donor cells to perform SCNT; then, cleavage rate, blastocyst rate, inner cell mass-to-trophectoderm ratio, and the expression of some development-and apoptosis-related genes in different groups were analyzed. The miR-101-2 suppressed the expression of inhibitor of growth protein 3 (ING3) at mRNA and protein levels, expedited cell proliferation, and decreased apoptosis in BFF, suggesting that ING3, a target gene of miR-101-2, is a potential player in this process. Moreover, by utilizing donor cells overexpressing miR-101-2, the development of bovine SCNT embryos in vitro was significantly enhanced; the apoptotic rate in SCNT blastocysts was reduced, and the inner cell mass-totrophectoderm ratio and SOX2, POU5F1, and BCL2L1 expression significantly increased, whereas BAX and ING3 expression decreased. Collectively, these findings suggest that miR-101-2 promotes BFF proliferation and vitality, reduces their apoptosis, and improves the early development of SCNT embryos.

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Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer

Cited by 8 publications

References 41 publications

Listening to mother: Long‐term maternal effects in mammalian development

Listening to mother: Long‐term maternal effects in mammalian development

Ovulation Statuses of Surrogate Gilts Are Associated with the Efficiency of Excellent Pig Cloning

Overexpression of miR-101-2 in donor cells improves the early development of Holstein cow somatic cell nuclear transfer embryos

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