Overexpression of miR-101-2 in donor cells improves the early development of Holstein cow somatic cell nuclear transfer embryos

Chang, Haoya; Xie, Rongxia; Zhang, Long; Fu, Liangzheng; Zhang, C.T.; Chen, H.H.; Wang, Z.Q.; Zhang, Yong; Quan, Fusheng

doi:10.3168/jds.2018-15072

Cited by 13 publications

(10 citation statements)

References 41 publications

(44 reference statements)

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“…The TUNEL assay was performed using the DeadEnd Fluorometric TUNEL system (Promega, Madison, WI) as previously described, with minor modifications (Chang et al, ). Blastocysts were washed in PBS containing 0.2% polyvinyl acetate (PVA‐PBS) and fixed with 4% paraformaldehyde for 2 hr at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Chang

Chen

Zhang

et al. 2019

Molecular Reproduction Devel

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As an assisted reproduction technology, vitrification has been widely used for oocyte and embryo cryopreservation. Many studies have indicated that vitrification affects ultrastructure, gene expression, and epigenetic status. However, it is still controversial whether oocyte vitrification could induce DNA damage in metaphase II (MII) oocytes and the resulting early embryos. This study determined whether mouse oocytes vitrification induce DNA damage in MII oocytes and the resulting preimplantation embryos, and causes for vitrification‐induced DNA damage. The effects of oocyte vitrification on reactive oxygen species (ROS) levels, γ‐H2AX accumulation, apoptosis, early embryonic development, and the expression of DNA damage‐related genes in early embryos derived by in vitro fertilization were examined. The results indicated that vitrification significantly increased the number of γ‐H2AX foci in zygotes and two‐cell embryos. Trp53bp1 was upregulated in zygotes, two‐cell embryos and four‐cell embryos in the vitrified group, and Brca1 was increased in two‐cell embryos after vitrification. Vitrification also increased the ROS levels in MII oocytes, zygotes, and two‐cell embryos and the apoptotic rate in blastocysts. Resveratrol (3,5,4′‐trihydroxystilbene) treatment decreased the ROS levels and the accumulation of γ‐H2AX foci in zygotes and two‐cell embryos and the apoptotic rate in blastocysts after vitrification. Overall, vitrification‐induced abnormal ROS generation, γ‐H2AX accumulation, an increase in the apoptotic rate and the disruption of early embryonic development. Resveratrol treatment could decrease ROS levels, γ‐H2AX accumulation, and the apoptotic rate and improve early embryonic development. Vitrification‐associated γ‐H2AX accumulation is at least partially due to abnormal ROS generation.

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Section: Methodsmentioning

confidence: 99%

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Chang

Chen

Zhang

et al. 2019

Molecular Reproduction Devel

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“…For miR-10a, targeting APAF1 in the embryo-development-related network, anti-apoptotic effects have been shown after delivery by exosomes to follicular cells [73]. Interestingly, miR-101 has been shown recently to improve the early development of bovine somatic cell nuclear transfer embryos when overexpressed in the donor cells by increasing proliferation and vitality, and improving the early embryonic development [74].…”

Section: Unveiling the Potential Of Oev Rna Components To Regulate Emmentioning

confidence: 99%

The Oviductal Extracellular Vesicles’ RNA Cargo Regulates the Bovine Embryonic Transcriptome

Bauersachs

Mermillod

Almiñana

2020

IJMS

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Oviductal extracellular vesicles (oEVs) are emerging as key players in the gamete/embryo–oviduct interactions that contribute to successful pregnancy. Various positive effects of oEVs on gametes and early embryos have been found in vitro. To determine whether these effects are associated with changes of embryonic gene expression, the transcriptomes of embryos supplemented with bovine fresh (FeEVs) or frozen (FoEVs) oEVs during in vitro culture compared to controls without oEVs were analyzed by low-input RNA sequencing. Analysis of RNA-seq data revealed 221 differentially expressed genes (DEGs) between FoEV treatment and control, 67 DEGs for FeEV and FoEV treatments, and minor differences between FeEV treatment and control (28 DEGs). An integrative analysis of mRNAs and miRNAs contained in oEVs obtained in a previous study with embryonic mRNA alterations pointed to direct effects of oEV cargo on embryos (1) by increasing the concentration of delivered transcripts; (2) by translating delivered mRNAs to proteins that regulate embryonic gene expression; and (3) by oEV-derived miRNAs which downregulate embryonic mRNAs or modify gene expression in other ways. Our study provided the first high-throughput analysis of the embryonic transcriptome regulated by oEVs, increasing our knowledge on the impact of oEVs on the embryo and revealing the oEV RNA components that potentially regulate embryonic development.

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“…The low full-term development rate of cloned embryos from somatic cell nuclear transfer (SCNT) is the result of inefficient reprogramming, as reflected by abnormal DNA hypermethylation and histone modifications. Although many efforts were applied to improve the success rate of SCNT in different species, the efficiency still remains low (Wakayama et al 1998, Ogura et al 2000, Wakayama & Yanagimachi 2001, Zhou et al 2003, Zhao et al 2009, Kim et al 2012, Chen et al 2015, Huang et al 2016, Wang et al 2017, Gao et al 2018, Chang et al 2019. However, when the cloned embryos were treated with the histone deacetylase inhibitor trichostatin A (TSA) after nuclear transfer, their preimplantation development as measured by the blastocyst rate, and the full-term development of these embryos was dramatically improved, indicating an important role of TSA in correcting reprogramming of the acetylation pattern, and converting a somatic nucleus from a differentiated state into a totipotent state (Kishigami et al 2006a, Rybouchkin et al 2006, Hai et al 2011, Saini et al 2014, Inoue et al 2015, Qiu et al 2017, Azuma et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of Trichostatin A on Mouse Embryogenesis and Development

Peng

Hai

et al. 2021

Reproduction

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Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, can significantly improve the reprogramming efficiency of somatic cells. However, whether TSA has detrimental effect on other kinds of embryos is largely unknown because of the lack of integrated analysis of TSA effect on natural fertilized embryos. To investigate the effect of TSA on mouse embryo development, we analyzed preimplantation and post-implantation development of in vivo, in vitro fertilized, and parthenogenetic embryos treated with TSA at different concentrations and durations. In vivo fertilized embryos appeared to be the most sensitive to TSA treatment among the three groups, and the blastocyst formation rate decreased sharply as TSA concentration and treatment time increased. TSA treatment also reduced the livebirth rate for in vivo fertilized embryos from 56.59% to 38.33%, but did not significantly affect postnatal biological functions such as the pups’ reproductive performance and their ability for spatial learning and memory. Further analysis indicated that the acetylation level of H3K9 and H4K5 was enhanced by TSA treatment at low concentrations, while DNA methylation appeared to be also disturbed by TSA treatment only at high concentration. Thus, our data indicates that TSA has different effects on preimplantation embryonic development depending on the nature of the embryo’s reproductive origin, the TSA concentration and treatment time, whereas the effect of TSA at indicated concentration on postnatal function was minor.

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Overexpression of miR-101-2 in donor cells improves the early development of Holstein cow somatic cell nuclear transfer embryos

Cited by 13 publications

References 41 publications

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

The Oviductal Extracellular Vesicles’ RNA Cargo Regulates the Bovine Embryonic Transcriptome

Differential Effects of Trichostatin A on Mouse Embryogenesis and Development

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