Treatment of Deep Cartilage Defects of the Knee with Autologous Chondrocyte Transplantation: Long-Term Results

Komárek, Jan; Vališ, Petr; Repko, Martin; Chaloupka, Richard; Krbec, Martin

doi:10.55095/achot2010/054

Cited by 28 publications

(2 citation statements)

References 19 publications

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“…Through the isolation and culture of cells, the scaffold provides temporary space and pre-constructed three-dimensional shape for cell growth, and finally forms regenerated cartilage. 18 However, the technique remains limited by several factors such as stress, microgravity, oxygen concentration and cytokines. 19–21 According to a previous study, 22 there was no significant difference in the effectiveness of three existing methods for the treatment of cartilage injury.…”

Section: Discussionmentioning

confidence: 99%

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Levels of matrix metalloproteinase-2 in committed differentiation of bone marrow mesenchymal stem cells induced by kartogenin

Wang

Liu

et al. 2019

J Int Med Res

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Objective To measure the inductive effect of kartogenin on matrix metalloproteinase-2 levels during the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes in vitro. Methods In vitro cultured bone marrow hMSCs were grown to the logarithmic phase and then divided into three groups: control group (0 µM kartogenin), 1 µM kartogenin group and 10 µM kartogenin group. After 72 h of culture, cell proliferation and differentiation were observed microscopically. Matrix metalloproteinase-2 (MMP-2) in the cell supernatant and type II collagen levels in the cells were detected by enzyme linked immunosorbent assay and immunofluorescence staining, respectively. Results Kartogenin induced the proliferation and differentiation of hMSCs. With the increase of kartogenin concentration, the level of type II collagen was increased, while the level of MMP-2 decreased. Conclusion These findings indicate that kartogenin can induce hMSCs to differentiate into chondrocytes, and with the increase of kartogenin concentration, degeneration of the cartilage extracellular matrix may be inhibited.

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Section: Discussionmentioning

confidence: 99%

“…In vitro and in vivo experiments have shown that KGN promotes chondrocyte differentiation and proliferation. 18 With a series of encouraging research results attracting the attention of scholars, 23–25 the role of KGN in the process of cartilage repair has been described as a ‘game changer’. 26…”

Section: Discussionmentioning

confidence: 99%