Treatment of chemically induced oral ulcer using adipose‐derived mesenchymal stem cell sheet

Lee, Doh Young; Kim, Hee Bok; Shim, In Kyong; Kanai, Nobuo; Okano, Teruo; Kwon, Seong Keun

doi:10.1111/jop.12517

Cited by 30 publications

(26 citation statements)

References 47 publications

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“…2A ), which was consistent with the clinical symptoms. CIM is another model applied for studying oral mucositis caused by cancer therapy 20 . Similarly, the expression levels of CXCL2 mRNA and protein were substantially increased in CIM (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic modification to induce CXCR2 overexpression in mesenchymal stem cells enhances treatment benefits in radiation-induced oral mucositis

et al. 2018

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Radiation-induced oral mucositis affects patient quality of life and reduces tolerance to cancer therapy. Unfortunately, traditional treatments are insufficient for the treatment of mucositis and might elicit severe side effects. Due to their immunomodulatory and anti-inflammatory properties, the transplantation of mesenchymal stem cells (MSCs) is a potential therapeutic strategy for mucositis. However, systemically infused MSCs rarely reach inflamed sites, impacting their clinical efficacy. Previous studies have demonstrated that chemokine axes play an important role in MSC targeting. By systematically evaluating the expression patterns of chemokines in radiation/chemical-induced oral mucositis, we found that CXCL2 was highly expressed, whereas cultured MSCs negligibly express the CXCL2 receptor CXCR2. Thus, we explored the potential therapeutic benefits of the transplantation of CXCR2-overexpressing MSCs (MSCsCXCR2) for mucositis treatment. Indeed, MSCsCXCR2 exhibited enhanced targeting ability to the inflamed mucosa in radiation/chemical-induced oral mucositis mouse models. Furthermore, we found that MSCCXCR2 transplantation accelerated ulcer healing by suppressing the production of pro-inflammatory chemokines and radiogenic reactive oxygen species (ROS). Altogether, these findings indicate that CXCR2 overexpression in MSCs accelerates ulcer healing, providing new insights into cell-based therapy for radiation/chemical-induced oral mucositis.

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Section: Resultsmentioning

confidence: 99%

Genetic modification to induce CXCR2 overexpression in mesenchymal stem cells enhances treatment benefits in radiation-induced oral mucositis

et al. 2018

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“…Hence, they have a good capability to differentiate toward both the adipogenic and the osteogenic lineage (Ardeshirylajimi et al, 2015; Ghaderi et al, 2018), providing a strong rationale for their use in maxillofacial surgery. Indeed, they have been adopted both for facilitating the healing of oral ulcers and for bone reconstruction procedures (Lee et al, 2017). Moreover, both harvesting and application of BFP into alveolar cavities or onto buccal soft tissues have proven to be safe and without any risk for the patient (Khojasteh & Sadeghi, 2016; Khojasteh et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Platelet‐rich plasma counteracts detrimental effect of high‐glucose concentrations on mesenchymal stem cells from Bichat fat pad

D’Esposito

Lecce

Marenzi

et al. 2020

J Tissue Eng Regen Med

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Diabetic patients display increased risk of periodontitis and failure in bone augmentation procedures. Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) represent a relevant advantage in tissue repair process and regenerative medicine. We isolated MSCs from Bichat's buccal fat pad (BFP) and measured the effects of glucose and PRP on cell number and osteogenic differentiation potential. Cells were cultured in the presence of 5.5-mM glucose (low glucose [LG]) or 25-mM glucose (high glucose [HG]). BFP-MSC number was significantly lower when cells were cultured in HG compared with those in LG. Following osteogenic differentiation procedures, calcium accumulation, alkaline phosphatase activity, and expression of osteogenic markers were significantly lower in HG compared with LG. Exposure of BFP-MSC to PRP significantly increased cell number and osteogenic differentiation potential, reaching comparable levels in LG and in HG. Thus, high-glucose concentrations impair BFP-MSC growth and osteogenic differentiation. However, these detrimental effects are largely counteracted by PRP. K E Y W O R D S Bichat's buccal fat pad, bone reconstruction, diabetes, mesenchymal stem cells, platelet-rich plasma Vittoria D'Esposito and Manuela Lecce equally contributed to this work.

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“…Within the last two decades, cell sheets of MSCs have been used in tissue engineering/tissue regeneration of several types of tissues and/or organs including (but are not limited to) the cornea [ 39 ], skin [ 40 , 41 ], cartilage [ 42 , 43 ], meniscus [ 44 ], bone [ 31 , 45 – 53 ], tendons [ 53 ], cardiac tissue [ 54 – 62 ], periodontal tissue [ 63 , 64 ], nasal epithelium [ 65 ], tooth [ 66 ], and blood vessel [ 67 ]. Cell sheets of MSCs have also been used in conditions such as wound healing [ 41 ], oral ulcers [ 68 ], digestive fistula [ 69 ], and spinal cord defects [ 70 , 71 ]. It has also been demonstrated that MSC sheets can induce angiogenesis in ischemic and/or wound tissues [ 41 , 72 ].…”

Section: Mesenchymal Stem Cells and Their Use In Cell Sheet-based mentioning

confidence: 99%

A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

Yörükoğlu

Kiter

Akkaya

et al. 2017

Stem Cells International

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The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.

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Treatment of chemically induced oral ulcer using adipose‐derived mesenchymal stem cell sheet

Abstract: Cell sheet technology using MSC can be an alternative treatment for oral ulcerations in that it can decrease healing time without invasive properties.

Cited by 30 publications

References 47 publications

Genetic modification to induce CXCR2 overexpression in mesenchymal stem cells enhances treatment benefits in radiation-induced oral mucositis

Genetic modification to induce CXCR2 overexpression in mesenchymal stem cells enhances treatment benefits in radiation-induced oral mucositis

Platelet‐rich plasma counteracts detrimental effect of high‐glucose concentrations on mesenchymal stem cells from Bichat fat pad

A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

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