Treatment of burns and chronic wounds using a new cell transfer dressing for delivery of autologous keratinocytes

Zhu, Ning; Warner, Richard C.; Simpson, C.; Glover, Mark; Hernon, Catherine; Kelly, J.; Fraser, Sarah; Brotherston, T. M.; Ralston, David R.; MacNeil, Sheila

doi:10.1007/s00238-005-0777-4

Cited by 47 publications

(37 citation statements)

References 19 publications

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“…These cell carriers have been used successfully in the clinic to deliver autologous keratinocytes for the treatment of patients with extensive skin loss resulting from burn injury and diabetic foot ulcers. 20,[22][23][24] Here, we demonstrate for the first time that these carriers are supportive of MSC cultures. The cultures retain their phenotype and ability to support vascular tubule formation after 72 h culture on the carriers, and the carriers allow the effective transfer of MSCs to human dermis model wound beds.…”

mentioning

confidence: 57%

“…Such dressings are currently being used in the clinic to deliver cultured autologous keratinocytes to patients with extensive burn injuries 23 and chronic wounds. 20,24 Given the potential therapeutic benefits that MSCs possess with respect to wound healing, it was of interest to determine whether similar surface preparations translate to the efficient delivery of these cells without altering the properties of the cells.…”

Section: Discussionmentioning

confidence: 99%

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A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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Skin has a remarkable capacity for regeneration, but age-and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds.

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mentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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show abstract

“…Another advantage is that proliferatively active keratinocytes could be delivered to the patient with greater time flexibility (Hernon et al 2006) that cannot be achieved with confluent cultured epithelial sheet grafts. This product is indicated for the treatment of neuropathic, pressure and diabetic foot ulcers, superficial burns and skin graft donor sites with reported positive clinical outcomes (Zhu et al 2005;Moustafa et al 2007). It can also be applied to full-thickness wounds in combination with meshed skin grafts but cannot be used alone for deep-wound treatment and in this way is similar to other epithelial bioengineered constructs.…”

Section: Myskinmentioning

confidence: 99%

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

617

469

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Situations where normal autografts cannot be used to replace damaged skin often lead to a greater risk of mortality, prolonged hospital stay and increased expenditure for the National Health Service. There is a substantial need for tissue-engineered skin bioconstructs and research is active in this field. Significant progress has been made over the years in the development and clinical use of bioengineered components of the various skin layers. Off-the-shelf availability of such constructs, or production of sufficient quantities of biological materials to aid rapid wound closure, are often the only means to help patients with major skin loss. The aim of this review is to describe those materials already commercially available for clinical use as well as to give a short insight to those under development. It seeks to provide skin scientists/tissue engineers with the information required to not only develop in vitro models of skin, but to move closer to achieving the ultimate goal of an off-the-shelf, complete full-thickness skin replacement.

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“…41,112 In general, there are four techniques: keratinocytes grown into integrated cell sheets, 53 made into suspensions or injections, 166 sprayed onto wound sites , 28,34 or delivered on various support carriers, scaffolds, or dressings. 162,164 Some of them are available commercially under different name brands.…”

Section: Cell Transplantation As Regenerative Therapy For the Skin-immentioning

confidence: 99%

Bioengineering the Skin–Implant Interface: The Use of Regenerative Therapies in Implanted Devices

Peramo

Marcelo

2010

Ann Biomed Eng

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This discussion and review article focuses on the possible use of regenerative techniques applied to the interfaces between skin and medical implants. As is widely known, the area of contact between an implant and the skin--the skin-implant interface--is prone to recurrent and persistent problems originated from the lack of integration between the material of the implant and the skin. Producing a long-term successful biointerface between skin and the implanted device is still an unsolved problem. These complications have prevented the development of advanced prosthetics and the evolution of biointegrated devices with new technologies. While previous techniques addressing these issues have relied mostly on the coating of the implants or the modification of the topology of the devices, recent in vitro developed techniques have shown that is possible to introduce biocompatible and possibly regenerative materials at the skin-device interface. These techniques have also shown that the process of delivering the materials has biological effects on the skin surrounding the implant, thus converting bioinert into bioactive, dynamic interfaces. Given that the best clinical outcome is the long-term stabilization and integration of the soft tissue around the implant, this article presents the basis for the selection of regenerative materials and therapies for long-term use at the skin-device interface, with focus on the use of natural biopolymers and skin cell transplantation.

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Treatment of burns and chronic wounds using a new cell transfer dressing for delivery of autologous keratinocytes

Cited by 47 publications

References 19 publications

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Bioengineering the Skin–Implant Interface: The Use of Regenerative Therapies in Implanted Devices

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