Controlling agricultural nonpoint source pollution from livestock grazing is a necessary step to improving the water quality of the nation's streams. The goal of enhanced stream water quality will most likely result from the implementation of an integrated system of best management practices (BMPs) linked with stream hydraulic and geomorphic characteristics. However, a grazing BMP system is often developed with the concept that BMPs will function independently from interactions among controls, climatic regions, and the multifaceted functions exhibited by streams. This paper examines the peer reviewed literature pertaining to grazing BMPs commonly implemented in the southern humid region of the United States to ascertain effects of BMPs on stream water quality. Results indicate that the most extensive BMP research efforts occurred in the western and midwestern U.S. While numerous studies documented the negative impacts of grazing on stream health, few actually examined the success of BMPs for mitigating these effects. Even fewer studies provided the necessary information to enable the reader to determine the efficacy of a comprehensive systems approach integrating multiple BMPs with pre‐BMP and post‐BMP geomorphic conditions. Perhaps grazing BMP research should begin incorporating geomorphic information about the streams with the goal of achieving sustainable stream water quality.

Due to the complex nature of pesticide transport, process-based models can be difficult to use. For example, pesticide transport can be effected by macropore flow, and can be further complicated by sorption, desorption and degradation occurring at different rates in different soil compartments. We have used the Root Zone Water Quality Model (RZWQM) to investigate these phenomena with field data that included two management conditions (till and no-till) and metribuzin concentrations in percolate, runoff and soil. Metribuzin degradation and transport were simulated using three pesticide sorption models available in RZWQM: (a) instantaneous equilibrium-only (EO); (b) equilibrium-kinetic (EK, includes sites with slow desorption and no degradation); (c) equilibrium-bound (EB, includes irreversibly bound sites with relatively slow degradation). Site-specific RZWQM input included water retention curves from four soil depths, saturated hydraulic conductivity from four soil depths and the metribuzin partition coefficient. The calibrated parameters were macropore radius, surface crust saturated hydraulic conductivity, kinetic parameters, irreversible binding parameters and metribuzin half-life. The results indicate that (1) simulated metribuzin persistence was more accurate using the EK (root mean square error, RMSE = 0.03 kg ha(-1)) and EB (RMSE = 0.03 kg ha(-1)) sorption models compared to the EO (RMSE = 0.08 kg ha(-1)) model because of slowing metribuzin degradation rate with time and (2) simulating macropore flow resulted in prediction of metribuzin transport in percolate over the simulation period within a factor of two of that observed using all three pesticide sorption models. Moreover, little difference in simulated daily transport was observed between the three pesticide sorption models, except that the EB model substantially under-predicted metribuzin transport in runoff and percolate >30 days after application when transported concentrations were relatively low. This suggests that when macropore flow and hydrology are accurately simulated, metribuzin transport in the field may be adequately simulated using a relatively simple, equilibrium-only pesticide model.

Surface mining is a common method for extracting coal in the coal fields of eastern Kentucky. Using the Forestry Reclamation Approach (FRA), which emphasizes the use of minimally compacted or loose-dumped spoil as a growth medium for trees, reclamation practitioners are successfully reestablishing forests. Yet, questions remain regarding the effects FRA has on the quality of waters discharged to receiving streams. To examine the effect of FRA on water quality, this study compared waters that were discharged from three types of spoils: predominantly brown, weathered sandstone (BROWN); predominantly gray, unweathered sandstone (GRAY); and an equal mixture of both aforementioned sandstones and shale (MIXED). The water quality parameters pH, EC, Ca, K, Mg, Na, NO-N, NH-N, SO, Cl, TC, suspended sediment concentration (SSC), settleable solids (SS), and turbidity were monitored over a 2-yr period on six 0.4-ha plots (two replications per spoil type). Generally, levels of Cl, SO, Ca, NO-N, NH-N, SS, SSC, and turbidity decreased over time. The pH for all spoils increased from about 7.5 to 8.5. The EC remained relatively level in the BROWN spoil, whereas the GRAY and MIXED spoils had downward trajectories that were approaching 500 μS cm. The value of 500 μS cm has been reported as the apparent threshold at which certain taxa such as Ephemeroptera (e.g., Mayfly) recolonize disturbed headwater streams of eastern Kentucky and adjacent coal-producing Appalachian states.

Six research plots were established on a surface mine for the purpose of evaluating the forest productivity potential and hydrological and water quality characteristics of three different loose-graded spoil types. The three spoil types were: (1) predominately brown, weathered sandstone (BROWN); (2) predominately gray, un-weathered sandstone (GRAY); and (3) mixed weathered and un-weathered sandstones and shale material (MIXED). The average area of the six plots was approximately 3,658 m 2 . The physical and chemical soil characteristics that gave the BROWN spoil type a predictably higher productivity potential and natural regeneration than the GRAY and MIXED spoil were its finer soil texture, higher CEC and P concentration, and a pH that was more suitable for native hardwood trees. Four species of tree seedlings were planted into the spoils. Growth and survival of the planted trees were evaluated for three years. As an indicator of natural succession potential, percentage ground cover of volunteer vegetation on the three spoil types was also evaluated. By the third year (2007) after planting, the BROWN spoil type had a significantly higher average tree volume index than the MIXED spoil and MIXED was significantly higher than GRAY. Ground cover from natural regeneration was found to be 66.4% on the BROWN spoil (61 different species), 5.8% on the MIXED spoil (35 different species), and less than 2.0% on the GRAY spoil (12 different species). Results showed that the loose-graded spoil in this experiment was characterized by low discharge volumes, small peak discharges, and long durations of discharge and had hydrologic characteristics of a forested watershed, even at this early stage of development. Generally, concentrations of Ca, Mg, and SO 4 2-decreased over time in GRAY and MIXED and increased in BROWN. The pH of the water discharge from all three spoil types has increased from about 7.5 to 8.5. Although the average electrical conductivity (EC) in water discharged from the BROWN spoil remained relatively level during the study period, the GRAY and MIXED appears to be on a downward trajectory from about 1500 µS cm -1 to about 500 µS cm -1 . The latter value of EC has been reported as the apparent threshold at which the benthic invertebrate community returns to drastically disturbed headwater streams of eastern Kentucky and adjacent coal-producing Appalachian states.

Surface mining has altered a vast land area in the Appalachian Region, threatening highly biodiverse native forest, contributing to habitat fragmentation, and generating severely disturbed sites that are unsuitable for succession to native ecosystems. Although there are many factors that influence species colonization and establishment on these sites, selection of topsoil substitutes suitable for native species is of particular concern. A series of experimental plots was installed in 2005 on a reclaimed mine site in eastern Kentucky, United States, to examine the suitability of three spoil types (unweathered GRAY sandstone, weathered BROWN sandstone, and MIXED sandstone/shale) as topsoil substitutes. Bareroot 1:0 seedlings of four native hardwood species (Fraxinus pennsylvanica, Quercus rubra, Q. alba, and Liriodendron tulipifera) were planted in the spoil. Seed required for ground cover was not applied so that natural colonization could be evaluated. Two years after installation, researchers concluded that tree growth was highest on BROWN; in addition, species richness and ground cover of volunteer vegetation were higher on BROWN. In 2013, tree volume was over 50 times higher in BROWN than GRAY. In addition to planted hardwoods, naturally colonizing vegetation provided nearly 100% cover on BROWN compared to 20% on MIXED and less than 10% on GRAY plots. Species richness of volunteer vegetation continued to be higher on BROWN (41) than GRAY (30) or MIXED (30), with native species comprising 65–70% of total species richness on all plots. Findings suggest that when topsoil substitutes are used, weathered spoils are more favorable to reforestation than unweathered spoil.

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Abstract. In 1996, a multidisciplinary group of researchers at the University of Kentucky initiated a study on the Starfire surface mine in eastern Kentucky to evaluate the effects of soil compaction and two organic amendments on the survivability and growth of high value tree species. Three types of prepared rooting medium were examined: compacted spoil, lightly compacted spoil, and uncompacted spoil. The compacted spoil was prepared using normally accepted spoil handling techniques that resulted in a smooth graded surface. The lightly compacted spoil was loose-dumped and struck-off with one or two passes of a bulldozer. The uncompacted spoil was loose-dumped and not further disturbed. In addition, organic amendments (mulches) were evaluated within the three reclamation techniques. The organic amendments used were processed hardwood bark mulch and a combination of straw and horse manure mulch. The following six species of trees were planted: white oak (Quercus alba), white ash (Fraxinus americana), eastern white pine (Pinus strobus), northern red oak (Quercus rubra), black walnut (Juglans nigra), and yellow poplar (Liriodendron tulipifera). Five of the six species, the exception being white ash, showed increased survivability as compaction was minimized. Additionally, the loose-graded techniques led to enhanced growth in height for the seedlings. The addition of organic amendments also showed additional benefit but results varied by species and by treatment. Results definitively show that strike-off and loose-dump techniques improve seedling height and survival. The data also suggest that even a small amount of traffic (i.e., one or two passes per the strike-off method) may result in enough compaction to significantly reduce survival and growth in some species, such as yellow poplar and white pine. In the backfilling and grading process, spoil material should be placed and compacted according to standard engineering practices so that the required stability and approximate original contour is achieved. However, the top 1.2 to 1.8 meters (4 to 6 feet) of material should not be graded or only lightly graded so that it is as uncompacted as possible.

The three-dimensional (3-D) arrangement of cells within tissues is integral to their development and function. Advances in stem cell science and regenerative medicine have stimulated interest in the replication of this architecture in vitro. We have developed a versatile method for controlling short-term cell-cell and cell-matrix interactions via a facile cell surface engineering process that enables the rapid formation of specific 3-D interactions for a range of cell types. We demonstrate that chemical modification of cell surfaces and matrix proteins can artificially accelerate the cell adhesion process and confirm the ability to control the formation of multicellular aggregates with defined architectures and heterotypic cell types. Direct comparison with a natural aggregation process seen during differentiation of embryonic stem (ES) cells revealed increased expression of developmental regulatory proteins and a concomitant enhancement of ES cell differentiation. Furthermore, this new methodology has numerous applications in generating layered structures. For example, we demonstrate improved transfer of therapeutic human keratinocytes onto a dermal layer in a skin repair model.