Treatment of boar sperm with nanoparticles for improved fertility

Feugang, Jean M.; Rhoads, Carley E.; Mustapha, Popoola A.; Tardif, Steve; Parrish, J.J.; Willard, Scott T.; Ryan, Peter L.

doi:10.1016/j.theriogenology.2019.05.040

Cited by 40 publications

(32 citation statements)

References 74 publications

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“…The present results indicated that beneficial effects of CU or CUNPs nanoparticles, at a high level (1 or 1.5 µg/mL) supplemented to the freezing medium, on post-thaw survival of rabbit spermatozoa via elevated the antioxidant capacity in seminal plasma. Hence, the nanoparticle has a promising strategy for using the maintenance of livestock sperm fertility during extended storage [ 16 ]. Besides, the use of POC as an indicator of oxidative stress has certain usefulness in contrast with the other oxidation parameters because of the relative early formation and relative stability of carbonylated proteins [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…It was confirmed that the bioavailability and absorption of some drugs were considerably improved when managed by nanoparticles [ 11 ]. Reports exhibited that the use of nanoparticles can enhance semen quality and freezability by decreasing the OS and protection the spermatozoa from cryo-damage [ 7 , 11 , 16 , 17 , 18 ]. Although, several previous studies have demonstrated that curcumin nanoparticles (CUNPs) could improve sperm kinetics, motion characteristics and testis function subjected to the induced OS in cryopreserved spermatozoa of different mammalian species [ 12 , 15 , 18 , 19 ]; however, no previous report explore the protective role of CUNPs in cryopreserved rabbit semen.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

Abdelnour

Hassan²,

Mohammed

et al. 2020

Animals

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The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

Abdelnour

Hassan²,

Mohammed

et al. 2020

Animals

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“…Interestingly, the current progress in nanotechnology is increasing the popularity of nanoparticles in biomedicine. These nanoparticles possess specific properties (e.g., sizedependent synthesis, bright and broad-spectrum fluorescence, biocompatibility and bioconjugation capability) that can be exploited for further investigation and improvement of reproductive outcomes [22,32,36]. In the present study, we used liposomes shaped like 192 extracellular membrane microvesicles (EVs), which are nano-and micro-size vesicles naturally released from cells to mediate a new type of cell-to-cell communication through passive interactions [17,18,37].…”

Section: Confocal Microscope Fluorescence Imaging Of Fwb Samples and mentioning

confidence: 99%

“…Liposomes are clinically relevant carbon-based 69 nanoparticles shaped like spherical vesicles constituted of phospholipid bilayers providing 70 biocompatibility, biodegradability, and low toxicity to cells [20]. These nanoparticles have been used as effective carriers for controlled and rapid deliveries of various hydrophilic and 72 hydrophobic molecules to specific biological sites, with indubitable benefits during in vivo and in 73 vitro applications [21,22]. For fertility treatments, several drugs, including biomolecules that are found in the FF [23][24][25][26][27], can be loaded into liposomes for intrafollicular injections.…”

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confidence: 99%

A Nano-Platform for in situ Investigations of Ovarian Follicles

Feugang

Ishak

Eggert

et al. 2020

Preprint

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Background: Despite the growing array of assisted reproductive techniques, there is still a lack of rapid, non-cytotoxic, and minimally invasive in situ approaches for further enhancements through cell targeting. Here we synthesized clinically relevant liposome nanoparticles for real-time cellular targeting and drug (doxorubicin) delivery, using pigs and mares as animal models. In Experiment 1, fluorescently labeled and doxorubicin-loaded (without fluorescent probe) liposomes were injected in cultured pig ovarian follicles to assess plasma membrane binding and intracellular doxorubicin delivery. In Experiment 2, fluorescent liposomes were in vivo injected into small and large ovarian follicles of living mares to assess their binding capability to follicular cells. Twenty-four hours post-injection, all cultured pig follicles were collected while mare samples (i.e., follicle wall fragments, granulosa cells and follicular fluids) were harvested through follicle wall biopsy (FWB), and follicle aspiration and flushing techniques using transvaginal ultrasound-guided approach.Results: All injected follicles were healthy and samples were subjected to fluorescence imaging before and after fixation. Findings revealed successful intrafollicular migration and binding of liposomes to all follicle cell layers (granulosa, theca interna, and theca externa) regardless of the follicle size. The intracellular delivery of doxorubicin was confirmed with the staining of nuclei of follicle cells. Conclusions: This study demonstrates the promising combination of the FWB technique and nanotechnology tools for real-time monitoring of intrafollicular treatment, follicular health and oocyte development, which in turn has the potential to help understand the mechanisms of ovulatory dysfunction and to select high-quatity oocytes for assisted reproduction techniques.

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“…The concept of this technology is to transform chemical molecules into small-scale size particles (range 1-200 nm). Consequently, new physical and chemical properties are achieved, including greater cellular uptake, reactivity, surface area and charge, and binding properties which might have opened a new platform for biosciences innovation [3,4]. Nano-sized drug delivery systems ( Figure 1) are being developed, both as therapeutic agents and as therapy delivery systems, because of the use of germicidal properties of metal and polymeric nanoparticles as antimicrobials, while natural and nanostructured materials can entrap and protect compounds for delivery [5].…”

Section: Introductionmentioning

confidence: 99%

State-of-the-Art and Prospective of Nanotechnologies for Smart Reproductive Management of Farm Animals

Hashem

González-Bulnes

2020

Animals

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Many biotechnological assisted reproductive techniques (ART) are currently used to control the reproductive processes of farm animals. Nowadays, smart ART that considers technique efficiency, animal welfare, cost efficiency and environmental health are developed. Recently, the nanotechnology revolution has pervaded all scientific fields including the reproduction of farm animals, facilitating certain improvements in this field. Nanotechnology could be used to improve and overcome many technical obstacles that face different ART. For example, semen purification and semen preservation processes have been developed using different nanomaterials and techniques, to obtain semen doses with high sperm quality. Additionally, nanodrugs delivery could be applied to fabricate several sex hormones (steroids or gonadotrophins) used in the manipulation of the reproductive cycle. Nanofabricated hormones have new specific biological properties, increasing their bioavailability. Applying nanodrugs delivery techniques allow a reduction in hormone dose and improves hormone kinetics in animal body, because of protection from natural biological barriers (e.g., enzymatic degradation). Additionally, biodegradable nanomaterials could be used to fabricate hormone-loaded devices that are made from non-degradable materials, such as silicon and polyvinyl chloride-based matrixes, which negatively impact environmental health. This review discusses the role of nanotechnology in developing some ART outcomes applied in the livestock sector, meeting the concept of smart production.

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Treatment of boar sperm with nanoparticles for improved fertility

Cited by 40 publications

References 74 publications

The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

A Nano-Platform for in situ Investigations of Ovarian Follicles

State-of-the-Art and Prospective of Nanotechnologies for Smart Reproductive Management of Farm Animals

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