Treatment of B16 mouse melanoma with the combination of electropermeabilization and chemotherapy

Heller, Richard; Jaroszeski, Mark J.; Leo-Messina, J.; Perrot, Rodolphe; Voorhis, N. Van; Ds, Reintgen; Gilbert, R. Alton

doi:10.1016/0302-4598(94)05013-k

Cited by 95 publications

(54 citation statements)

References 17 publications

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“…However, electric pulses delivered alone did not elicit an anti-tumour response. Anti-tumour effects were observed in many different animal models Salford et al, 1993;Mir, 1994;Ser'sa et al, 1994;1995;Yamaguchi et al, 1994;Cemazar et al, 1995;Heller et al, 1995). Anti-tumour treatment with this combined therapy can be used in tumour systems in which bleomycin alone was not typically used .…”

Section: Carcinomamentioning

confidence: 99%

Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy

Mir

Glass²,

Serša³

et al. 1998

Br J Cancer

414

233

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Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy LM Mir', LF Glass23, G Sersa4, J Teissi65, C Domenge6, D Miklavdid7, MJ Jaroszeski38, S Orlowski9, DS Reintgen38, Z Rudolf4, M Belehradek6, R Gilbertl0, M-P Rols5, J Belehradek Jr', JM Bachaud", R DeConti23, B Stabuc4, M Cemazar4, P Coninx'2 and R Heller38 The application of electric pulses to the patients was safe and well tolerated. An instantaneous contraction of the underlying muscles was noticed. Minimal adverse side-effects were observed. ECT was shown to be an effective local treatment. ECT was effective regardless of the histological type of the tumour. Therefore, ECT offers an approach to the treatment of cutaneous and subcutaneous tumours in patients with minimal adverse side-effects and with a high response rate.

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Section: Carcinomamentioning

confidence: 99%

Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy

Mir

Glass²,

Serša³

et al. 1998

Br J Cancer

414

233

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“…For the 12 cats of the treated group, BLM at a dose of 0.5 mg kg-' was injected intravenously in a bolus that lasted 30 s. This dose, similar on a body weight basis to that used in other preclinical and clinical trials Salford et al, 1993;Heller et al, 1994;Sersa et al, 1994;Yamaguchi et al, 1994), is totally ineffective in the absence of the potentializing EP. The nodules of the first cats and the thin nodules of other cats were treated using the procedure previously tested in other animal species as well as in humans Mir et al, 1991Mir et al, , 1992, i.e.…”

Section: Treatment Procedures Using Externally Delivered Electric Pulsesmentioning

confidence: 99%

“…Thus, 100 js and 1300 V cm-' EPs were delivered at 1 Hz, in runs of four or eight pulses, through two external stainless-steel electrodes (A or B) placed over the treated nodule (Table 2). In the absence of BLM, these EPs are totally ineffective Heller et al, 1994;Sersa et al, 1994;Yamaguchi et al, 1994). In the electrochemotherapy sessions, all the EPs were delivered in less than 25 min, starting roughly 4 min after BLM administration.…”

Section: Treatment Procedures Using Externally Delivered Electric Pulsesmentioning

confidence: 99%

“…We have termed the new anti-tumour treatment, which combines systemic BLM and permeabilizing EPs locally delivered at the tumour site, electrochemotherapy Mir et al, 1991). Electrochemotherapy is effective in eradicating subcutaneously transplanted small tumours Serka et al, 1994;Heller et al, 1994;Yamaguchi et al, 1994). Good antitumour responses were also obtained in a murine spontaneous tumour model and with internally transplanted tumours (Salford et al, 1993).…”

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confidence: 99%

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First clinical trial of cat soft-tissue sarcomas treatment by electrochemotherapy

Mir

Devauchelle²,

Quintin-Colonna³

et al. 1997

Br J Cancer

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Summary Electrochemotherapy combines bleomycin and local electric pulses that allow cell permeabilization and free access of bleomycin to its intracellular target. We report the first veterinarian clinical trial of electrochemotherapy in 12 cats with spontaneous large soft-tissue sarcomas that suffered relapse after treatment with conventional therapies. Permeabilizing electric pulses were delivered using external surface electrodes, as well as new needle-shaped electrodes that were designed to be inserted in tumours for more effective treatment of several-centimetre-thick tumour nodules. The electric pulses were applied to the tumours several times from 4 to 15-30 min after a bolus intravenous injection of 0.5 mg kg-1 bleomycin. Tolerance to treatment was excellent without general side-effects. The cats showed local inflammatory reactions for a few days and disease stabilization lasted from 2 weeks to 7 months. One partial regression was observed, and the general absence of nodule volume decrease can be explained by local fibrotic reactions. Histological analysis of biopsies also revealed massive tumour cell death. The cats' lifespan increased (P40.001), with a mean survival time of 6.1 months (maximum 18 months) compared with 0.8 months (maximum 1.5 months) for a group of 11 untreated control cats displaying similar carcinological features. Electrochemotherapy is clearly effective as a salvage treatment for large spontaneous solid tumours in adverse clinical situations and this is promising for future applications.

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“…Electroporation has been used in vivo, in animals and patients [11][12][13], primarily to introduce anti-tumor drugs into cancer cells. Given the in vitro efficiency of this method of gene transfer, it appeared as a potentially efficient approach to target in vivo transfection.…”

Section: Introductionmentioning

confidence: 99%

In vivo gene electroinjection and expression in rat liver

et al. 1996

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In vivo targeted gene transfer by non-viral vectors is subjected to anatomical constraints depending on the route of administration. Transfection efficiency and gene expression in vivo using non-viral vectors is also relatively low. We report that in vivo electropermeabilization of the liver tissue of rats in the presence of genes encoding luciferase or ~-galactosidase resulted in the strong expression of these genetic markers in rat liver cells. About 31)-40% of the rat liver cells electroporated expressed the ~galactosidase genetic marker 48 h after electroporation. The marker expression was also detected at least 21 days after transfection at about 5% of the level 48 h after electroporation. The results indicate that gene transfer by electroporatinn in vivo may avoid anatomical constraints and low transfection efficiency.

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Treatment of B16 mouse melanoma with the combination of electropermeabilization and chemotherapy

Cited by 95 publications

References 17 publications

Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy

Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy

First clinical trial of cat soft-tissue sarcomas treatment by electrochemotherapy

In vivo gene electroinjection and expression in rat liver

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