In vivo gene electroinjection and expression in rat liver

Heller, Richard; Jaroszeski, Mark J.; Atkin, Andrew J.; Moradpour, Darius; Gilbert, R. Alton; Wands, Jack R.; Nicolau, Claude

doi:10.1016/0014-5793(96)00590-x

Cited by 393 publications

(233 citation statements)

References 13 publications

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“…These data corroborate previous studies that short length ( s) pulses efficiently enhance delivery of DNA to tissues such as normal hepatocytes 12 and rat brain tumor cells. 14 Longer length (ms) pulses also result in increased expression of injected plasmids to mouse testes, 15 rat hepatocytes, 13 mouse melanoma cells 16 and mouse skeletal muscle, [17][18][19] but tissue damage is observed at field strengths greater than 100 V/cm.…”

Section: (A) Psv-␤gal Injection Only; (B) Injection Of Psv-␤gal Follosupporting

confidence: 91%

“…[17][18][19] A previous study by this laboratory demonstrated in vivo gene delivery to normal liver tissue using electroporation. 12 In the present study, the delivery of plasmids encoding reporter genes to a solid visceral tumor is demonstrated. Reporter genes were used to evaluate delivery of plasmid DNA to rat hepatocellular carcinomas.…”

mentioning

confidence: 88%

“…8 This same pulsing protocol enhanced luciferase expression nine-fold over plasmid DNA injection alone in normal liver tissue. 12 In rat hepatocellular carcinomas, the application of a similar pulsing protocol resulted in a 15-fold increase in luciferase activity over simple injection ( Figure 1). Thus for rat hepatocellular carcinomas, the same pulsing protocol used to deliver chemotherapeutic agents effectively enhanced plasmid DNA delivery in vivo.…”

mentioning

confidence: 97%

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Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

et al. 2000

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Section: (A) Psv-␤gal Injection Only; (B) Injection Of Psv-␤gal Follosupporting

confidence: 91%

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confidence: 88%

mentioning

confidence: 97%

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Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

et al. 2000

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“…20 Furthermore, it was observed that a very efficient in vivo electroloading of large molecules other than plasmids was obtained for proteins, 19 dextran, 21 and antisense oligonucleotides. 22 Electrically mediated gene transfer had been shown to be effective on many tissues: liver, 23 skin, 24 muscle 21,25 and heart. 26 Delivery is targeted to the volume where the field pulse is applied, that is, under the control of the electrode localization.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

et al. 2004

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Owing to their capacity to induce strong, sequence-specific, gene silencing in cells, short interfering RNAs (siRNAs) represent new potential therapeutic tools. This development requires, however, new safe and efficient in vivo siRNA delivery methods. In the present technical report, we show that electrically mediated siRNA transfer can suppress transgene expression in adult mice muscles. Using electropulsation for siRNA delivery opens the way for a targeted gene silencing on a broad range of tissues. Clinical applications of electropulsation for delivery of other classes of molecules are under trials. We reported that gene silencing was efficiently obtained in vivo in an adult mammal (mouse) with chemically synthesized siRNA after its electrical delivery. The associated gene silencing was followed on the same animal and lasted at least 11 days. Gene silencing was obtained in muscles not only on young adult mice but also on much older animals. No tissue damages were detected under our electrical conditions. Therefore, this method should provide an efficient approach for a localized delivery of siRNAs in various tissues and organs. Gene Therapy (2005) 12, 246-251.

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“…22 However, in vivo electroporation has so far received little attention. [23][24][25] It has largely been used only in cell suspensions, since a high-voltage electric pulse is necessary to complete efficient gene transfer in vitro, and high voltages are harmful to animal and human tissues. Zheng et al 26 reported, however, that gene transfer could be accomplished efficiently using electric pulses in attached cells at lower voltages than those required for cell-suspension transfers.…”

Section: Possible Mechanismsmentioning

confidence: 99%

Targeted gene transfer to corneal endothelium in vivo by electric pulse

et al. 1998

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A novel method of in vivo targeted gene transfer to intenthelial cells as early as day 1 and lasted until day 21. The tionally selected areas of the corneal endothelium was most intense gene expression was observed on days 1 and developed. Plasmid DNA with the lacZ gene coding for ␤-3 (5.21% on day 1 and 6.45% on day 3). The expression galactosidase was injected into the anterior chamber of of ␤-galactosidase on day 3 was most evident following adult Wistar rats, and eight pulses of electricity at intendelivery of 20 V electric pulses (0.09% at 5 V, 0.03% at sities ranging from 5 to 40 V/cm were delivered for 50 ms 10 V, 6.45% at 20 V). ␤-Galactosidase expression was limto the cornea with a specially designed electric probe in ited to the corneal endothelial cells in highly selected areas order to determine the effect of gene transfer on the corand no ␤-galactosidase expression was detected in any neal endothelial cells. Gene expression was visualized by other intra-or extraocular tissues. In addition, no cell damenzymatic color reaction using X-gal in enucleated eyes on age was apparent in the cornea and no inflammation was days 1, 3, 7, 14 and 21 after gene transfer. The treated detected in any other intraocular tissues. Thus, low-voltage eyes were then photographed and the X-gal-positive areas electric pulses successfully transferred the gene of interest were evaluated by an image analyzer. The ratios of the to highly selective areas of the corneal endothelium without areas (X-gal-positive area/area of entire corneal endoinducing any pathological changes. This targeted gene thelium × 100%) were then calculated to determine gene transfer method appears to have great potential for use in transfection efficiency. The expression of ␤-galactosidase gene therapy for ocular diseases. was clearly detected in the cytoplasm of the corneal endo-

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In vivo gene electroinjection and expression in rat liver

Cited by 393 publications

References 13 publications

Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

Targeted gene transfer to corneal endothelium in vivo by electric pulse

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