Treatment of androgenetic alopecia by exosomes secreted from hair papilla cells and the intervention effect of LTF

Abstract

BackgroundAndrogenetic alopecia (AGA) is the most common cause of chronic progressive hair loss in men, and AGA has a severe negative impact on the quality of life and physical and mental health of patients.MethodsFour female C57BL/6 mice were isolated from DP cells in culture (≤4 generations) after stimulation of DPC proliferation by herbal concentrations obtained by the CCK‐8 method, and exosomes were isolated by differential centrifugation at low temperature. Testosterone propionate and topical hair removal treatments were used together to establish the C57BL/6 mouse AGA model, which was treated with LTF, 5% minoxidil, and LTF‐DPC‐EXO, respectively. ELISA was used to detect serum hormone levels, in vivo tracing was used to observe dynamic changes in exosomes, H&E staining showed changes in mouse hair follicle tissue, and (q) RT‐PCR and WB were used to detect dorsal skin VEGF, AKT1, and CASP3 expression in dorsal skin tissues.ResultsHair regeneration was significant in the LTF group, minoxidil group, and LTF‐DPC‐EXO group mice, and the hair growth was only seen in the local skin in the model group. The hormone T in all treatment groups was lower than that in the model group, and e2 was higher than that in the model group. (q) RT‐PCR and western blot showed that VEGF and AKT1 expressions were upregulated and Caspase3 expression was downregulated in the skin sections of mice in the treatment groups.ConclusionDPC‐EXO obtained through LTF may activate AKT1 and VEGF in the PI3K/AKT signaling pathway to inhibit CASP3, thereby protecting DPC to restore the hair growth.

Medical Treatment for Androgenetic Alopecia

“…The study concluded that DPC-Exos contribute to the regulation of HF growth and development, and provide a potential avenue for the treatment of hair loss. 98 In summary, interest in extracellular vesicles is increasing both aimed at understanding their biological functions and using them towards practical applications such as non-invasive diagnostic technique and advanced therapeutic drug delivery system. These in vitro and in vivo findings have implications for the development of therapeutic strategies for hair loss.…”