Treating Uveitis in the Developing World Setting

Rathinam, Sivakumar R.

doi:10.1097/iio.0b013e3181d2d7b1

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…Laboratory support is crucial for a definitive diagnosis of dengue, chikungunya, or WNV. 19 In the present study, of 27 WNV patients, six did not have any systemic illness and two had a severe systemic illness with viral meningitis. During the previous outbreak of chikungunya viral infection in South India in 2005/2006, constitutional symptoms and arthralgia were very common clinical signs.…”

Section: Discussionmentioning

confidence: 50%

Molecular detection and characterization of West Nile virus associated with multifocal retinitis in patients from southern India

Shukla

Saxena

Rathinam

et al. 2012

International Journal of Infectious Diseases

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Section: Discussionmentioning

confidence: 50%

Molecular detection and characterization of West Nile virus associated with multifocal retinitis in patients from southern India

Shukla

Saxena

Rathinam

et al. 2012

International Journal of Infectious Diseases

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“…Uveitis is one of the most harmful ocular conditions and can affect any part of the eye. In developed countries, uveitis affects ~200/100,000 people in the general population, and 35% of patients suffer severe visual impairment ( 1 , 2 ). Endotoxin-induced uveitis (EIU) is an animal model of human uveitis induced by lipopolysaccharide (LPS), a component of the outer membranes of gram-negative bacteria ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis

Jiang

Qiu

Lou

et al. 2016

Molecular Medicine Reports

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Neutrophils have been recognized as critical response cells during the pathogenesis of endotoxin-induced uveitis (EIU). Apoptosis of neutrophils induced by roscovitine has previously been demonstrated to ameliorate inflammation in several in vivo models. The present study aimed to assess whether roscovitine ameliorates EIU. EIU was induced in female C57BL/6 mice by a single intravitreal injection of lipopolysaccharide (LPS; 250 ng). The mice were divided into three groups as follows: LPS alone, LPS plus vehicle, LPS plus roscovitine (50 mg/kg). The mice were euthanized 12, 24, 48 and 72 h after LPS-induced uveitis. Accumulation of inflammatory cells in the vitreous body was confirmed by immunohistochemistry, and quantified following hematoxylin and eosin staining. Terminal deoxynucleotidyl transferase dUTP nick-end labeling was performed to detect of apoptotic cells. The mRNA levels of inflammatory cytokines were analyzed by reverse transcription-quantitative polymerase chain reaction and the changes in protein levels were analyzed by western blotting. Inflammatory cells accumulated in the vitreous near the optic nerve head and the quantity peaked at 24 h after LPS injection. Immunohistochemistry revealed that the majority of the inflammatory cells were neutrophils. The number of infiltrating cells was similar in the LPS and LPS plus vehicle groups, while there were significantly less in the roscovitine group at 24 h. Apoptosis of neutrophils was observed between 12 and 48 h after roscovitine injection, while no apoptosis was observed in the other groups. The mRNA expression levels of GMCSF, CINC-1 and ICAM-1 peaked at 12 h after LPS injection, and decreased to normal levels at 72 h. This trend in mRNA expression was similar in the LPS and LPS plus vehicle groups; however, the expression levels decreased more quickly in the roscovitine group at 24 and 48 h. Following roscovitine administration, upregulated cleaved caspase 3 expression levels and downregulated Mcl-1 expression levels were observed. In conclusion, roscovitine ameliorates EIU by effecting neutrophil apoptosis. Timely apoptosis of neutrophils may be an effective process to promote the amelioration of EIU.

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“…Infectious uveitis is common, particularly in referral centers and in the developing world, where up to onethird or more of all patients with ocular inflammation have an identified infectious cause for their uveitis. [1][2][3][4][5] In addition, less direct contributions of infections to the development or continuation of ocular inflammation can also occur. [6][7][8][9][10] Perhaps best recognized is the so-called Jarisch-Herxheimer Reaction (JHR), named after Adolf Jarisch and Karl Herxheimer, two dermatologists who independently described transient worsening of syphilitic skin lesions following treatment with mercury in 1895 and 1902, respectively.…”

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confidence: 99%