Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs

Sakita, Gabriel Zanuto; Lima, Paulo de Mello Tavares; Filho, Adibe Luiz Abdalla; Bompadre, Thiago Francisco Ventoso; Ovani, V.S.; Chaves, Camila de Miranda e Silva; Bizzuti, Beatriz Elisa; Costa, Wilian dos Santos da; Paim, Tiago do Prado; Campioni, Tania Sila; Neto, Pedro de Oliva; Bremer‐Neto, Hermann; Louvandini, Hélder; Abdalla, Adibe Luiz

doi:10.1016/j.anifeedsci.2022.115253

Cited by 11 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to this, Pinos-Rodriguez et al [74] confirmed in an in situ and in vivo study that EFE (Fibrozyme) has no significant (P>0.05) effect on feed intake of ruminally cannulated Ramboulliet lambs which were fed TMR with different forage:concentrate (F:C) ratios. Similar results were obtained in lambs by Sakita et al [75]. Furthermore, Bueno et al [76] supplemented EFE (Fibrozyme) to lambs @ 0, 5 and 10 g enzyme per kg of forage respectively along with the basal diet and observed no significant difference (P>0.05) in average daily gain and feed conversion ratio; the only linear effect was observed (P=0.04) with respect to feed intake.…”

Section: Small Ruminantssupporting

confidence: 76%

“…Sheikh et al [71] observed that feeding of complete feed to Corriedale Sheep prepared from urea molasses treated paddy straw (T2) and exogenous enzyme (9 g/kg DM) plus urea molasses treated paddy straw (T3) significantly (P<0.01) increased digestibility of DM, CP, NDF, ADF and cellulose in T3 group which is followed by T2 and T1 (control). While, digestibility of NFE and hemicellulose was similar in all the groups.Treating tifton-85 hay with fibrolytic enzymes extract 24 hours before feeding to lambs resulted 12% higher ADF digestibility [75].…”

Section: Small Ruminantsmentioning

confidence: 93%

See 1 more Smart Citation

A Review on the Role of Exogenous Fibrolytic Enzymes in Ruminant Nutrition

Anil

Yadav²,

Anand³

et al. 2022

CJAST

View full text Add to dashboard Cite

Dairy production system in developing countries mainly depends on forage plants and crop residues as major portion of the Ruminants diet. The majority of the dry matter in forage crops is made up of fibre whose digestibility is limited in rumen ecosystem. Use of exogenous fibrolytic enzymes (EFE) is gaining popularity in recent days as they overcome the limitations of other methodologies which are used to improve the digestibility of fibre. Due to microbial enzyme activity, ruminants are able to break down fibrous feedstuff, but structural polysaccharides like cellulose, hemicellulose, and lignin will only be partially broken down. The primary purpose of these enzymes is to provide as many nutrients as possible from the indigestible, potentially digestible, and digestible portions of the cell wall. EFE employed in ruminant diets can be divided into three primary categories based on the specific substrates on which their enzyme activity can take place: fibrolytic, amylolytic, and proteolytic. Enzymes can be applied in liquid or granular form to hay, silage, concentrate, TMR, supplement or premix. Even though positive results were obtained, up to date animal responses to EFE supplements have varied greatly due to enzymatic handling, dosage, diet constituents, time and method of applications. This renders need for further dedicated research efforts for the broad generalization of exogenous enzyme usage in ruminant nutrition. The goal of this study was to give a concise summary of the current state of knowledge about EFE usage in ruminant diets and to discuss potential future research areas.

show abstract

Section: Small Ruminantssupporting

confidence: 76%

Section: Small Ruminantsmentioning

confidence: 93%

A Review on the Role of Exogenous Fibrolytic Enzymes in Ruminant Nutrition

Anil

Yadav²,

Anand³

et al. 2022

CJAST

View full text Add to dashboard Cite

show abstract

“…Another limitation of respiration chambers is that animals may not exhibit normal behaviors, e.g., feed consumption may decrease, thus resulting in an under-estimation of actual CH 4 emissions compared to free-ranging animals under farm conditions ( Huhtanen et al, 2019 ). In research studies, animals usually undergo a metabolism or performance trial, and CH 4 is measured over 3 to 5 consecutive days by moving the trained animals to the chambers ( Sakita et al, 2022 ). Several factors are essential when using this technology for controlled experiments, such as gas recovery routine maintenance, chamber temperature (<27 °C), relative humidity (<90%), CO 2 concentration (<0.5%), ventilation rate (VR; 250 to 260 L/min), and constant gas flow as suggested by Pinares-Patiño and Waghorn (2014) .…”

Section: Measurements Using Animal-based Techniquesmentioning

confidence: 99%

“…Alternative low-cost systems exist ( Abdalla et al, 2012 ; Hellwing et al, 2012 ; Canul Solis et al, 2017 ) that use the same principles as open-circuit indirect calorimetry, but with lower-cost materials available locally and simplified air-conditioning systems. These alternative chambers are typically set up in the daily environment of the cow ( Hellwing et al, 2012 ; Canul Solis et al, 2017 ) or sheep ( Abdalla et al, 2012 ; Sakita et al, 2022 ). With open-circuit chambers, gas concentrations are measured in the outlet using an infrared analyzer, gas chromatograph, or laser.…”

Section: Measurements Using Animal-based Techniquesmentioning

confidence: 99%

Quantification of methane emitted by ruminants: a review of methods

Tedeschi

Abdalla

Álvarez

et al. 2022

Journal of Animal Science

Self Cite

View full text Add to dashboard Cite

The contribution of greenhouse gas (GHG) emissions from ruminant production systems varies between countries and between regions within individual countries. The appropriate quantification of GHG emissions, specifically methane (CH4), has raised questions about the correct reporting of GHG inventories and, perhaps more importantly, how best to mitigate CH4 emissions. This review documents existing methods and methodologies to measure and estimate CH4 emissions from ruminant animals and the manure produced therein over various scales and conditions. Measurements of CH4 have frequently been conducted in research settings using classical methodologies developed for bioenergetic purposes, such as gas exchange techniques (respiration chambers, headboxes). While very precise, these techniques are limited to research settings as they are expensive, labor-intensive, and applicable only to a few animals. Head-stalls, such as the GreenFeed system, have been used to measure expired CH4 for individual animals housed alone or in groups in confinement or grazing. This technique requires frequent animal visitation over the diurnal measurement period and an adequate number of collection days. The tracer gas technique can be used to measure CH4 from individual animals housed outdoors, as there is a need to ensure low background concentrations. Micrometeorological techniques (e.g., open-path lasers) can measure CH4 emissions over larger areas and many animals, but limitations exist, including the need to measure over more extended periods. Measurement of CH4 emissions from manure depends on the type of storage, animal housing, CH4 concentration inside and outside the boundaries of the area of interest, and ventilation rate, which is likely the variable that contributes the greatest to measurement uncertainty. For large-scale areas, aircraft, drones, and satellites have been used in association with the tracer flux method, inverse modeling, imagery, and lidar, but research is lagging in validating these methods. Bottom-up approaches to estimating CH4 emissions rely on empirical or mechanistic modeling to quantify the contribution of individual sources (enteric and manure). In contrast, top-down approaches estimate the amount of CH4 in the atmosphere using spatial and temporal models to account for transportation from an emitter to an observation point. While these two estimation approaches rarely agree, they help identify knowledge gaps and research requirements in practice.

show abstract

“…Agricultural activity is facing many challenges such as the need to increase food production to meet the growing world population [ 1 ] while adapting to environmental and economic changes by improving animal performance in more sustainable production systems [ 2 ]. Among the issues surrounding the growth of this sector is the increased use and degradation of natural resources, directly contributing to worsening the global climate change scenario due to greenhouse gas (GHG) emissions, depleting water resources, causing soil erosion and impairing natural habitats [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Pigeon Pea Intercropped with Tropical Pasture as a Mitigation Strategy for Enteric Methane Emissions of Nellore Steers

Furtado

Filho

Bruno

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

In this study, we evaluate the effects of intercropping pigeon pea (Cajanus cajan (L.) Millsp.) with tropical pastures for feeding Nellore cattle and compared animal performance and enteric CH4 emissions with other pasture-based systems during the dry and rainy seasons of 2021. Thirty-six Nellore steers (with a body weight of 221 ± 7 kg and an age of 15–16 months) were randomly distributed in three treatments with three replicates (in paddocks of 1.5 hectares each): (1) a degraded pasture of Urochloa spp. (DEG); (2) a recovered and fertilized pasture of Urochloa spp. (REC); and (3) pigeon pea intercropped with Urochloa spp. (MIX). Enteric CH4 emissions were estimated using the sulfur hexafluoride (SF6) tracer gas technique, and dry matter intake (DMI) was determined using internal (iNDF) and external (TiO2) markers. Forages were collected by hand plucking after observations of ingestive behavior, and feces was collected after voluntary defecation. The proportion of grass and legume intake was estimated by C stable isotopes, and the forage nutritional quality was determined, while animal performance was monitored monthly, and the stocking rate was adjusted by the “put and take” technique. The results indicated that intercropping pigeon pea with tropical grasses is an interesting strategy for sustainable livestock production based on pastures. The MIX treatment was able to meet the nutritional requirements of the animals, which presented higher performance. In addition, there was a reduction in CH4 emissions up to 70% when expressed per average daily weight gain in comparison to the DEG treatment.

show abstract

Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs

Cited by 11 publications

References 63 publications

A Review on the Role of Exogenous Fibrolytic Enzymes in Ruminant Nutrition

A Review on the Role of Exogenous Fibrolytic Enzymes in Ruminant Nutrition

Quantification of methane emitted by ruminants: a review of methods

Pigeon Pea Intercropped with Tropical Pasture as a Mitigation Strategy for Enteric Methane Emissions of Nellore Steers

Contact Info

Product

Resources

About