Treating Soil PCP at Optimal Conditions Using Heme and Peroxide

Chen, Shyi Tien; Stevens, David King; Kang, Guyoung; Hsieh, Ming-Chia

doi:10.1061/(asce)0733-9372(2006)132:7(704)

Cited by 10 publications

(13 citation statements)

References 14 publications

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“…The extent of 14 CO 2 evolution during treatment was measured by LS-6500 liquid scintillation counter (Beckman) as described previously [13][14]. After 14 CO 2 evolution experiment, 14 C remaining in soil samples was extracted with dichloromethane for 24 h using Soxhlet procedure. The extracted solvent was concentrated to be solvent-exchanged with acetonitrile.…”

Section: Degradation Of Pyrene By Hemoglobin-catalyzed Reactionmentioning

confidence: 99%

“…One mL of 2 mL solvent sample was used to measure 14 C extent of sample solvent by LS-6500 liquid scintillation counter. For the remaining soil after Soxhlet procedure, 14 C extent of solvent non-extractable minerals was determined [11,17].…”

Section: Degradation Of Pyrene By Hemoglobin-catalyzed Reactionmentioning

confidence: 99%

“…The other 1 mL of 2 mL sample solvent was subjected to HPLC. A part of HPLC eluent was used to measure the extent of radioactivity with a Beckman LS-6500 liquid scintillation counter [13,14]. The other part of HPLC eluent was extracted with hexane to be concentrated to 1 mL with a solvent concentrator.…”

Section: Pyrene Degradation Intermediatesmentioning

confidence: 99%

“…Thus, organic radicals are generated during the catalytic cycle of Hb in the presence of H 2 O 2 . These organic radicals might participate in chemical reactions such as oxidation and polymerization, during which various intermediate products can be formed during detoxification of contaminants [10][11][12][13][14][15]. In our recent study with Hb-catalyzed reaction, when pyrene in liquid solution is oxidatively removed, hydrophilic degradation intermediates are formed [15].…”

Section: Introductionmentioning

confidence: 99%

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Analyses of intermediate products during degradation of pyrene in soil by hemoglobin-catalyzed reaction

Keum

Kang

2018

JABC

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Hemoglobin (Hb) is a member of heme-protein that can perform catalytic non-specific chain reaction in the presence of hydrogen peroxide (H 2 O 2). Catalytic ability of Hb to degrade pyrene was demonstrated using soil contaminated with 14 C pyrene and 10 mg pyrene /kg soil. The composition of soil was similar to previously used soil except that it had lower organic carbon content. Bench scale laboratory tests were conducted in the presence of buffer only, H 2 O 2 only, or Hb with H 2 O 2 for 24 h. After 24 h reaction, 0.1 and 1.3% of 14 C pyrene in contaminated soil were mineralized with H 2 O 2 only or Hb plus H 2 O 2. No mineralization to 14 CO 2 was detected with buffer only. Approximately 12.2% of pyrene was degraded in the presence of H 2 O 2 only while 44.0% of pyrene was degraded in the presence of Hb plus H 2 O 2 during 24 h of catalytic reaction. When degradation intermediate products were examined, two chemicals were observed in the presence of H 2 O 2 only while 25 chemicals were found in the presence of Hb plus H 2 O 2. While most degradation products were simple hydrocarbons, four of the 27 chemicals had aromatic rings. However, none of these four chemicals was structurally related to pyrene. These results suggest that Hb catalytic system could be used to treat pyrene-contaminated soil as an efficient and speedy remediation technology. In addition, intermediate products generated by this system are not greatly affected by composition change in soil organic matter content.

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Section: Degradation Of Pyrene By Hemoglobin-catalyzed Reactionmentioning

confidence: 99%

Section: Degradation Of Pyrene By Hemoglobin-catalyzed Reactionmentioning

confidence: 99%

Section: Pyrene Degradation Intermediatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analyses of intermediate products during degradation of pyrene in soil by hemoglobin-catalyzed reaction

Keum

Kang

2018

JABC

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“…Among the remediation strategies for treating TPH-contaminated soils, combining chemical and biological processes has been shown to effective (Palmroth etal., 2006), Using abiotic processes, hydrogen peroxide has been widely used to degrade xenobiotics in slurry systems requiring large amounts of hydrogen peroxide, and the generated hydroxyl radical is strongly reactive and toxic to microorganisms (Danner et al, 1973;Umezawa and Higuchi, 1989;Palmroth et al, 2006;Oh and Shin, 2013) A wide range of pentachlorophenol (PCP), polycyclic aromatic hydrocarbons (PAHs), lipids, and fatty acids are catalytically oxidized by heme and heme proteins (Rice et al, 1983;Chen et al, 2006;Chen et al, 2009;Chung et al, 2014;Kang et al, 2015). By analogy to the peroxidase system, it was verified that adding hydrogen peroxide to soil containing heme, in which the iron is present as Fe…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biodegradation of Total Petroleum Hydrocarbons (TPHs) in Contaminated Soil using Biocatalyst

Owen¹,

Pyo²,

Kang³

2015

Journal of Soil and Groundwater Environment

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Biocatalytic degradation of total petroleum hydrocarbons (TPHs) in contaminated soil by hemoglobin and hydrogen peroxide is an effective soil remediation method. This study used a laboratory soil reactor experiment to evaluate the effectiveness of a nonspecific biocatalytic reaction with hemoglobin and H 2 O 2 for treating TPH-contaminated soil. We also quantified changes in the soil microbial community using real-time PCR analysis during the experimental treatment. The results show that the measured rate constant for the reaction with added hemoglobin was 0.051/day, about 3.5 times higher than the constant for the reaction with only H 2 O 2 (0.014/day). After four weeks of treatment, 76% of the initial soil TPH concentration was removed with hemoglobin and hydrogen peroxide treatment. The removal of initial soil TPH concentration was 26% when only hydrogen peroxide was used. The soil microbial community, based on 16S rRNA gene copy number, was higher (7.1 × 10 6 copy number/g of bacteria, and 7.4 × 10 5 copy number/g of Archaea, respectively) in the hemoglobin catalyzed treatment. Our results show that TPH treatment in contaminated soil using hemoglobin catalyzed oxidation led to the enhanced removal effectiveness and was non-toxic to the native soil microbial community in the initial soil.

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Degradation of Pyrene Contaminated Soil with Spiked 14C Pyrene by Hemoglobin Catalysis

et al. 2018

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Treating Soil PCP at Optimal Conditions Using Heme and Peroxide

Cited by 10 publications

References 14 publications

Analyses of intermediate products during degradation of pyrene in soil by hemoglobin-catalyzed reaction

Analyses of intermediate products during degradation of pyrene in soil by hemoglobin-catalyzed reaction

Enhanced Biodegradation of Total Petroleum Hydrocarbons (TPHs) in Contaminated Soil using Biocatalyst

Degradation of Pyrene Contaminated Soil with Spiked 14C Pyrene by Hemoglobin Catalysis

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