Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy

Okamoto, Naoya; Kushida, Taketoshi; Oe, Kenichi; Umeda, Mikio; Ikehara, Susumu; Iida, Hiroya

doi:10.2106/jbjs.i.01325

Cited by 95 publications

(105 citation statements)

References 40 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…This finding is corroborated by the study by Okamoto et al (2010), who reported that MSCs promote the release of growth factors and cytokines, thus increasing local vasculariza-tion in addition to acting as an extrinsic factor for tendon healing. Moreover, the suture also increases vascularization, given its foreign body reaction (Manson and Allen, 1941).…”

Section: Discussionsupporting

confidence: 73%

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. Rupture of the Achilles tendon diminishes quality of life. The gold-standard therapy is a surgical suture, but this presents complications, including wound formation and inflammation. These complications spurred evaluation of the therapeutic potential of 10435 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 10434-10449 (2014) Stem cells for tendon injury treatment mesenchymal stem cells (MSCs) from adipose tissue. New Zealand rabbits were divided into 6 groups (three treatments with two time points each) evaluated at either 14 or 28 days after surgery: cross section of the Achilles tendon (CSAT); CSAT + Suture; and CSAT + MSC. A comparison between all groups at both time points showed a statistically significant increase in capillaries and in the structural organization of collagen in the healed tendon in the CSAT + Suture and CSAT + MSC groups at the 14-day assessment. Comparison between the two time points within the same group showed a statistically significant decrease in the inflammatory process and an increase in the structural organization of collagen in the CSAT and CSAT + MSC groups. A study of the genomic integrity of the cells suggested a linear correlation between an increase of injuries and culture time. Thus, MSC transplantation is a good alternative for treatment of Achilles tendon ruptures because it may be conducted without surgery and tendon suture and, therefore, has no risk of adverse effects resulting from the surgical wound or inflammation caused by nonabsorbable sutures. Furthermore, this alternative treatment exhibits a better capacity for wound healing and maintaining the original tendon architecture, depending on the arrangement of the collagen fibers, and has important therapeutic potential.

show abstract

Section: Discussionsupporting

confidence: 73%

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

et al. 2014

View full text Add to dashboard Cite

show abstract

“…To this end, direct cell injections were pioneered, with positive results in equine patients [44,214], even with low number of cells [215][216][217]. However, direct cell injections have failed to deliver in a consistent manner in humans due to poor cell localisation [218][219][220][221], triggering an extensive investigation into the optimal cell carrier for tendon repair [1]. The ideal carrier system should prevent cell membrane rupture during the injection process; create increased tissue integration through fast in situ self-assembly; facilitate long-term cell survival and functionality maintenance; and allow spatiotemporal release of the cargo [222][223][224][225][226][227][228][229][230][231].…”

Section: Delivery Of Viable Cell Populationsmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

172

188

View full text Add to dashboard Cite

“…Injections of MSCs into midsubstance Achilles tendon ruptures increased ultimate failure load in a rat model [103,104]. Huang et al found that failure load for the normoxic MSC group was 2.7 N/mm 2 at 4 weeks vs. 1.7 N/ mm 2 for untreated controls.…”

Section: Stem Cells Bone Marrow Stem Cellsmentioning

confidence: 99%

“…Okamoto also found MSCs to be more effective than bone marrow cells (BMCs). Ultimate failure loads following MSC treatment were 3.8 N vs. 0.9 N for controls and 2.1 N for bone marrow cell-treated tendons (p < 0.016) [104]. Nourissat et al [11] removed the enthesis of the Achilles tendon in a rat model.…”

Section: Stem Cells Bone Marrow Stem Cellsmentioning

confidence: 99%

Biologic and Tissue Engineering Strategies for Tendon Repair

Sigal

Grande

Dines

et al. 2016

Regen. Eng. Transl. Med.

View full text Add to dashboard Cite

This review summarizes recent developments in biologic treatments-including growth factors, platelet-rich plasma (PRP), stem cells, and cell-seeded scaffolds-for tendon repair. Growth and differentiation faction-5 (GDF-5), insulin-related growth factor-1 (IGF-1), and basic fibroblast growth factor (bFGF) all improved extracellular matrix (ECM) production and tensile strength of treated tendons; however, no clinical trials were done on GDF-5. Platelet-derived growth factor-BB (PDGF-BB) improved proliferation and ECM production, but did not consistently improve mechanical properties. The literature was mixed on the efficacy of PRP for the treatment of chronic and acute tendinopathies. However, PRP did cause any complications, and its benefits may be enhanced once an ideal, standardized composition is developed. Therefore, PRP may be a valid treatment, especially once nonsurgical management options have failed. Mesenchymal stem cells (MSCs) significantly and substantially improved the quality of tendon repairs and demonstrated the ability to regenerate an enthesis. Adipose-derived stem cells (ADSCs) have similar effects and are easier to harvest. The periosteum may also regenerate the tendon-bone attachment. Tenocytes, meanwhile, may be ideal for midsubstance tendon repairs. Cell-seeded scaffolds-especially ECMderived scaffolds-were demonstrated to improve ECM production, enhancing the healing abilities of tenocytes or stem cells while providing early mechanical support to healing tendons.Each of these treatments demonstrated enhanced healing compared to common surgical techniques; moreover, patient outcomes may be enhanced by combining these treatments. Lay SummaryTendon injuries are very prevalent and can be debilitating. Tendon heals poorly, and the scar tissue that forms is weak and susceptible to reinjury. A major focus of orthopedic research is regenerative medicine, encouraging the formation of healthy tendon rather than mechanically inferior scar tissue. This review summarizes the recent scientific literature on biologic treatments for tendon repair, such as growth factors, platelet-rich plasma, and stem cells. The purpose of which is to show which treatments are promising candidates for clinical use and research, helping to guide physicians and to lay out a path for future research.

show abstract

Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy

Abstract: Transplantation of whole bone marrow cells may be a better and more readily available treatment for Achilles tendon rupture than cultured mesenchymal stem cells.

Cited by 95 publications

References 40 publications

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

The past, present and future in scaffold-based tendon treatments

Biologic and Tissue Engineering Strategies for Tendon Repair

Contact Info

Product

Resources

About