The past, present and future in scaffold-based tendon treatments

Lomas, Alex; Ryan, Colin; Sorushanova, Anna; Shologu, Naledi; Sideri, Aikaterini; Tsioli, V.; Fthenakis, George C.; Tzora, Athina; Skoufos, Ioannis; Quinlan, Leo R.; ÓLaighin, Gearóid; Mullen, Anne Maria; Kelly, Jack L.; Kearns, Stephen; Biggs, Manus; Pandit, Abhay; Zeugolis, Dimitrios I.

doi:10.1016/j.addr.2014.11.022

Cited by 171 publications

(183 citation statements)

References 468 publications

(322 reference statements)

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“…As such, repair of larger tissue defects likely involves cells from the epitenon and endotenon that migrate into the wound [23,183]. The coordinated activities of cells from these two compartments has been suggested to promote optimal healing [184], and extrinsic compartment involvement in healing is often demonstrated as extensive vascular and nerve outgrowth from the peritenon into the tendon proper [19,[184][185][186][187][188]. Here the tissue barriers between the intrinsic and extrinsic compartments are violated, following a classic wound healing paradigm that includes bleeding, clot formation, recruitment of immune and progenitor cells to an assembly of granulation tissue, early tissue remodeling, and finally late tissue remodeling that should ideally involve resolution and retreat of neo-vasculature and neo-innervation (Fig.…”

Section: Tendon Damage and Repair: Intrinsic Microdamage Vs Damage Cmentioning

confidence: 99%

“…However, injured tendon often does not fully attain ''normal" levels of tissue quality and function, particularly in torn tendons with pre-existing tissue degeneration [5,167]. (Right): Following acute injury, the extrinsic healing system (vasculature, nerve, and immune system) is heavily involved in early stages of wound healing [19,[184][185][186][187][188]. Ideally, the involvement of these systems resolves over time, with vascular system suppression perhaps being tightly coordinated by the intrinsic tendon compartment.…”

Section: Unmet Clinical Needs and The Role Of Biomaterials In Addresmentioning

confidence: 99%

“…Here the tissue barriers between the intrinsic and extrinsic compartments are violated, following a classic wound healing paradigm that includes bleeding, clot formation, recruitment of immune and progenitor cells to an assembly of granulation tissue, early tissue remodeling, and finally late tissue remodeling that should ideally involve resolution and retreat of neo-vasculature and neo-innervation (Fig. 9) [9,18,187]. Inflammation plays a key role in the early stages of healing, with intricate coordination and cross-talk between the tendon core and the vascular, nervous, We extend Arnoczky's paradigm of damage-mediated tissue remodeling to consider in detail how tendon damage leads to shunting of mechanical loads to the remaining intact tissues that may provoke a (dys)functional cellular response [43,275,276].…”

Section: Tendon Damage and Repair: Intrinsic Microdamage Vs Damage Cmentioning

confidence: 99%

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Tendon injury and repair – A perspective on the basic mechanisms of tendon disease and future clinical therapy

2017

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Section: Tendon Damage and Repair: Intrinsic Microdamage Vs Damage Cmentioning

confidence: 99%

Section: Unmet Clinical Needs and The Role Of Biomaterials In Addresmentioning

confidence: 99%

Section: Tendon Damage and Repair: Intrinsic Microdamage Vs Damage Cmentioning

confidence: 99%

See 1 more Smart Citation

Tendon injury and repair – A perspective on the basic mechanisms of tendon disease and future clinical therapy

2017

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“…Given that tissue graft based therapies have failed to restore native tendon function, it is anticipated that the tissue-engineering arpeggio (scaffolds, cells, biologics alone or in combination) would provide a functional therapy in the years to come [1][2][3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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“…Tissue engineering of tendon can potentially overcome some of these deficiencies and promote the healing of tendon injury [9,10]. One of the key components of tendon tissue engineering is the scaffold.…”

Section: Introductionmentioning

confidence: 99%

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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The past, present and future in scaffold-based tendon treatments

Cited by 171 publications

References 468 publications

Tendon injury and repair – A perspective on the basic mechanisms of tendon disease and future clinical therapy

Tendon injury and repair – A perspective on the basic mechanisms of tendon disease and future clinical therapy

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

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