Transvaginal ultrasound-guided oocyte retrieval following FSH stimulation of domestic goats

Graff, K.J.; Meintjes, M.; Dyer, V.W.; Paul, J.B.; Denniston, R.S.; Ziomek, Carol A.; Godke, R.A.

doi:10.1016/s0093-691x(99)80014-4

Cited by 50 publications

(21 citation statements)

References 57 publications

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“…Collection of oocytes from donors was performed by follicular puncture with needles guided by laparascopy, based on the procedure described by Graff et al. (1999).…”

Section: Methodsmentioning

confidence: 99%

“…2001). Graff et al. (1999) compared both procedures in goats and they found that transvaginal aspiration in spite of harvesting a half of oocytes available and being less efficient than transabdominal aspiration of follicles, it produced less adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…(1999) compared both procedures in goats and they found that transvaginal aspiration in spite of harvesting a half of oocytes available and being less efficient than transabdominal aspiration of follicles, it produced less adhesion. Laparascopy‐guided follicular puncture doubles the efficiency of oocyte collection, but the adhesions reported by Graff et al. (1999) may limit their use on donors.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Fertilization and Development of OPU Derived Goat and Sheep Oocytes

Cox

Alfaro

2007

Reprod Domestic Animals

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The recent upgrade in IVP technology seen in cattle can be adapted to embryo production in small ruminants to overcome limitations exhibited by surgical procedures on preserving the reproductive potential of donors and the efficiency of embryo production. The aim of the present study was to assess the current procedures used in cattle for the production of IVP embryos in goats and sheep based on laparoscopic-aided ovum pick-up (LOPU) supplied oocytes. Sexually matured goat and sheep donors were treated during the breeding season with FSH and subjected to laparoscopic-guided follicular puncture under general anaesthesia. The collected cumulus-oocyte complexes were matured in medium 199 and fertilized by frozen-thawed spermatozoa using Talp medium supplemented with heparin and oestrus-sheep serum. Cleaved ova were either cultured in sheep in vitro fertilization medium plus amino acids or transferred to sheep oviducts. Blastocyst rate, hatching rate and development rate up to term were used as markers of embryo function. The results obtained for goat and sheep involving 30 and 35 donors respectively (10 and 9 LOPU sessions) were 81.2% and 85.2% of oocyte collection rate; 88.3% and 98.6% oocyte incubation rate; 85.6% and 76.0% fertilization rate; 82.4% and 93.4% of cleavage rate; 50.0% and 61.5% IVP blastocyst rate; 42.1% and 45.5% blastocyst rate in oviducts; 73.0% and 66.7% embryo survival up to term, respectively. The results are comparable to those obtained in small ruminants and in bovines suggesting that requirements for embryo production and development are similar.

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“…Collection of oocytes from donors was performed by follicular puncture with needles guided by laparascopy, based on the procedure described by Graff et al. (1999).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Fertilization and Development of OPU Derived Goat and Sheep Oocytes

Cox

Alfaro

2007

Reprod Domestic Animals

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“…1997), laparoscopic follicular aspiration (Kuhholzer et al . 1997) or transvaginal ultrasound‐guided methods (Graff et al . 1999).…”

Section: Resultsmentioning

confidence: 99%

Caprine somatic cell nuclear transfer using in vivo matured oocytes collected by laparoscopic follicular aspiration

Ohkoshi

Takahashi

Koyama

et al. 2003

Animal Science Journal

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In vivo matured oocytes collected by laparoscopic follicular aspiration (LFA) from hormone treated female goats were used as recipient ooplasts for somatic cell nuclear transfer (SCNT). Japanese native (Shiba) goats were used as donor females and some donor females were used repeatedly (two or three times) at intervals of a few months. To induce synchronization of estrus, a sponge containing 0.5 g of progesterone was inserted into the vagina of each goat for 14 days. These animals were also treated with follicle stimulating hormone (FSH) in a series of 8 injections over 4 days. The first FSH injection was administered on the morning of day 9 of sponge insertion. On the morning of day 13, 50 µg of gonadotropin‐releasing hormone (GnRH) was injected into each animal. Twenty‐nine hours after GnRH injection, LFA was performed. After removal of cumulus cells, collected oocytes with the first polar body were selected and enucleated for nuclear transfer. Anterior pituitary cells isolated from an adult male Shiba goat were transfected with a DNA fragment containing the enhanced green flourescent protein gene and the puromycin resistance gene. A single donor cell was inserted into the perivitelline space of each enucleated oocyte and fusion was induced with one electric pulse of 20 V for 10 µs. The SCNT goat eggs were cultured in chemically defined medium at 38.5°C in 5% CO2, 5% O2, 90% N2 for 9 days. By LFA, 396 oocytes were collected from a total of 30 females. After removal of cumulus cells, 64% of them extruded the first polar body. The percentage of SCNT goat eggs produced using in vivo matured oocytes which developed to the blastocyst stage (20–21%) was significantly higher (P < 0.05) than that produced with in vitro matured oocytes (3–8%).

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“…TUGA has been extensively tested in cattle; cycling cows beef [13,16,18] dairy [4,6,33], pregnant cows [23], post-partum cows [27,28], pre-pubertal calves [7,9,30] and cows with reproductive problems [15,20]. The procedure has also been used in oocyte production in the cyclic mare [10,11,14] and pregnant mares [22], the goat [17], zebra [24], swamp buffalo [26], llama [8] and hoof-stock [21]. Historically, manual rectal palpation in the sow had met with limited application as compared with that in the cow and mare.…”

mentioning

confidence: 99%

Development of a Chute to Facilitate Transvaginal Ultrasound Guided Oocyte Aspiration (TUGA) in the Sow

Ferguson

Bellows²,

LeMieux

et al. 2013

J. Vet. Med. Sci.

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ABSTRACT. A procedure developed for nonsurgical transvaginal ultrasound-guided oocyte aspiration in the mature sow was dependent upon development of a special chute. This chute was designed to immobilize and protect the sow. The floor of the chute possessed a metal bar that was elevated via a hydraulic lift until the weight of the sow rested on the bar, while her hooves were still in contact with the floor. This exact positioning of the female prevented her from resisting procedure and allowed for a comfortable setting for technician to manipulate the ovaries (per rectum) and perform transvaginal ultrasound guided oocyte aspiration (TUGA). Oocyte collection was performed in six sows, and none experienced detrimental health effects from the procedure.

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Transvaginal ultrasound-guided oocyte retrieval following FSH stimulation of domestic goats

Cited by 50 publications

References 57 publications

In Vitro Fertilization and Development of OPU Derived Goat and Sheep Oocytes

In Vitro Fertilization and Development of OPU Derived Goat and Sheep Oocytes

Caprine somatic cell nuclear transfer using in vivo matured oocytes collected by laparoscopic follicular aspiration

Development of a Chute to Facilitate Transvaginal Ultrasound Guided Oocyte Aspiration (TUGA) in the Sow

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