<i>In Vitro</i> Fertilization and Development of OPU Derived Goat and Sheep Oocytes

Cox, JF; Alfaro, Vicente

doi:10.1111/j.1439-0531.2006.00735.x

Cited by 43 publications

(35 citation statements)

References 29 publications

(76 reference statements)

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“…Furthermore, the rate of oocyte recovery in prior studies were within 40 to 90% (Cognié et al, 2004;Morton et al, 2005;Rodriguez et al, 2006;Cox and Alfaro, 2007;Gibbons et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Cordeiro

Teixeira

Oliveira

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration [ ABSTRACTThe objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.

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“…Furthermore, the rate of oocyte recovery in prior studies were within 40 to 90% (Cognié et al, 2004;Morton et al, 2005;Rodriguez et al, 2006;Cox and Alfaro, 2007;Gibbons et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Cordeiro

Teixeira

Oliveira

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…Indeed, in vitro maturation (IVM) of cumulus-oocytes complexes (COCs) is a key step in improving the outcome of ARTs. Wellestablished culture systems used for IVM have substantially influenced the maturation rate, in vitro fertilization (IVF) rate, and subsequent embryonic development rate (Brackett and Zuelke, 1993;Cox and Alfaro, 2007;Combelles et al, 2009;Souza-Fabjan et al, 2014;Fakruzzaman et al, 2015). Under in vivo conditions, COCs are provided with all essential minerals, growth factors, proteins, and natural buffering agents that cannot be mimicked totally under in vitro counterparts.…”

Section: Introductionmentioning

confidence: 99%

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Veshkini¹,

Mohammadi‐Sangcheshmeh²,

Ghanem³

et al. 2018

Anim Reprod

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Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 mg/ml than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJtreated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was upregulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts.

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“…Estes autores utilizaram o mesmo protocolo de estimulação folicular relatado até o momento e obtiveram 18,4 ± 1,1 folículos por doadoras adultas e 32,0 ± 3,7 por doadoras pré-púberes, resultado superior aos obtidos neste trabalho, mostrando a possível influência da idade na atividade ovariana. Os 28,3 folículos por ovelhas Dorper e 22,1 folículos por ovelhas Santa Inês encontrados nesta pesquisa foram superiores aos resultados de Rodriguez et al (13) com 17,4 folículos, Cox & Alfaro (14) com 17,2 folículos, Wieczoreck et al…”

Section: Resultsunclassified

Aspiração Folicular Videolaparoscópica Comparativa Em Ovelhas Dorper E Santa Inês

Araújo

Oliveira

Tabet³

et al. 2016

Ciênc. anim. bras.

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ResumoEste estudo teve por objetivo comparar o número de folículos aspirados, oócitos recuperados, qualidade e viabilidade oocitária entre ovelhas das raças Dorper e Santa Inês, submetidas à aspiração folicular por vídeolaparoscopia. Para realização deste estudo, foram efetuadas 118 aspirações foliculares, das quais 65 foram realizadas em ovelhas da raça Dorper e 53 em ovelhas da raça Santa Inês. Os resultados encontrados para as ovelhas Dorper foram 28,31 ± 17,42 folículos/ovelha, 12,21 ± 6,74 oócitos/ovelha, taxa de recuperação de 47,9% e 8,93 ± 5,25 oócitos viáveis/ovelha. Para as ovelhas Santa Inês foram, 22,14 ± 16,08 folículos/ovelha, 12,64 ± 8,29 oócitos/ovelha, taxa de recuperação de 55% e 8,92 ± 6,58 oócitos viáveis/ovelha. Não houve influência das raças sobre o número de folículos aspirados, oócitos recuperados, taxa de recuperação, qualidade e viabilidade oocitária em aspiração folicular por vídeolaparoscopia. Palavras-chave: folículo; oócito; ovino. AbstractThis study aimed to compare the number of aspirated follicles, recovered oocytes, oocyte quality and viability between Dorper and Santa Inês ewes submitted to the videolaparoscopic ovum pick-up. To conduct this study, 118 follicular aspirations were performed, of which 65 were performed in Dorper ewes and 53 in Santa Ines ewes. The results for Dorper sheep were 28.31 ± 17.42 follicles/ewe, 12.21 ± 6.74 oocytes/ewe, recovery rate of 47.9% and 8.93 ± 5.25 viable oocytes/ewe. For Santa Inês ewes the results were 22.14 ± 16.08 follicles/ewe, 12.64 ± 8.29 oocytes/ewe, recovery rate of 55% and 8.92 ± 6.58 viable oocytes/ewe. There was no influence of breed on the number of aspirated follicles, recovered oocytes, recovery rate, oocyte quality and viability in videolaparoscopic ovum pick-up.

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In Vitro Fertilization and Development of OPU Derived Goat and Sheep Oocytes

Cited by 43 publications

References 29 publications

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Aspiração Folicular Videolaparoscópica Comparativa Em Ovelhas Dorper E Santa Inês

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