Transurethral versus suprapubic catheterization to test urethral function in rats

Abstract

Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.

A 15-year retrospective review of urodynamic studies in children at Red Cross War Memorial Children’s Hospital, Cape town, South Africa

“…The concern regarding transurethral catheters is that it can affect urethral function and increase leak point pressure (LPP). However, several studies have demonstrated that the use of catheterization does not alter urethral function [22][23][24]. The ICCS Standardization Report on Urodynamic Studies of the Lower Urinary Tract in Children recommends the use of either methods; however, risks should be weighed against benefit when the suprapubic route is used.…”

Assessing Neurogenic Lower Urinary Tract Dysfunction after Spinal Cord Injury: Animal Models in Preclinical Neuro-Urology Research

“…For instance, while some mouse cystometric studies have estimated the flow rate during voiding [ 181 , 211 ], the current assessment of urine flow in mice commonly depends on the observation of urine output and increased bladder pressure, thereby hindering the ability to distinguish coordinated voiding from non-voiding urine flow (i.e., involuntary urine expulsion associated with detrusor overactivity). Although transurethral catheterization does not appear to affect urethral function, as assessed during rodent CMG [ 212 ], small PE-50 catheters are prone to blockage [ 212 ]. In addition, longitudinal assessment is more challenging with the chronic catheter and electrode implantation commonly performed in small animals (e.g., inflammation, urinary tract infection, stone formation, bleeding, and catheter blockade) [ 158 , 171 ] compared to transurethral approaches.…”

Study of the Results of Multipotent Stromal Cell Adsorption on Polymer Materials Used in Urology

“…Ургентная инконтиненция составляет 22-36%, смешанная -29%, на долю других типов приходится от 5% до 10%. Стандартные методы лечения часто обеспечивают симптоматическое облегчение, но не нацелены на основную этиологию и демонстрируют огромную вариабельность результатов от пациента к пациенту, ограниченный успех и осложнения, связанные с процедурами [1,2].…”