Study of the Results of Multipotent Stromal Cell Adsorption on Polymer Materials Used in Urology

Abstract

The subcutaneous tissue of rats in various times after implantation of polypropylene materials used in urology, after passive adsorption of multipotent stromal cells (MSCs) with membranes labeled with Vybrant® CM-Dil, was study by methods of fluorescent microscopy. 1 week after implantation of both prolene monofilament and polypropylene mesh, numerous inclusions of different sizes and shapes with very intense fluorescence were found in large tissue cells near the foreign body when a rhodamine filter was applied. One month after the operation, only rare single objects with brighter luminescence were found when using a filter for rhodamine. It is most likely that large cells containing fluorescent inclusions are macrophages, and objects with luminescence when using a rhodamine filter are the detritus of injected MSCs with labeled Vybrant® CM-DiI membranes. Thus, almost all adsorbed MSCs undergo destruction in the tissues of the implantation site 1 week after the surgery, and by 1 month, the detritus of these MSCs is completely eliminated from the tissues. Thus, all effects of MSC adsorption on the surface of polymeric materials should be transient and limited to only 1 week, next the effect of cellular technologies on the inflammation, vascularization and structural organization of tissues surrounding a foreign body will most likely be invisible.