Transposition-Based Plant Transformation

Hua, Yan; Rommens, Caius M.

doi:10.1104/pp.106.090126

Cited by 8 publications

(5 citation statements)

References 44 publications

(44 reference statements)

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“…1). Although regeneration and transformation frequencies for this conventional-type vector approached that for pSIM108 (83.2% and 40.5%, respectively), we found that removal of the ipt gene lowered the frequency of backbone-free transformation by about six fold to similar levels as determined previously for the conventional vector pSIM401 (Yan and Rommens 2007) (Table 1). Regeneration of transformed plants on hormonefree media…”

Section: High Backbone-free Transformation Frequencies With Vectors Csupporting

confidence: 61%

Cytokinin vectors mediate marker-free and backbone-free plant transformation

et al. 2008

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Conventional Agrobacterium-mediated transformation methods rely on complex and genotype-specific tissue culture media for selection, proliferation, and regeneration of genetically modified cells. Resulting transgenic plants may not only contain selectable marker genes but also carry fragments of the vector backbone. Here, we describe a new method for the production of transgenic plants that lack such foreign DNA. This method employs vectors containing the bacterial isopentenyltransferase (ipt) gene as backbone integration marker. Agrobacterium strains carrying the resulting ipt gene-containing "cytokinin" vectors were used to infect explants of various Solanaceous plant species as well as canola (Brassica napus). Upon transfer to hormone-free media, 1.8% to 9.9% of the infected explants produced shoots that contained a marker-free T-DNA while lacking the backbone integration marker. These frequencies often equal or exceed those for backbone-free conventional transformation.

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Section: High Backbone-free Transformation Frequencies With Vectors Csupporting

confidence: 61%

Cytokinin vectors mediate marker-free and backbone-free plant transformation

et al. 2008

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“…This problem has raised potential biosafety issues related to environmental concerns and human health risks. To overcome these potential risks, methods of developing marker free transgenic plants have been developed, such as cotransformation [77], transposon-mediated transformation [78] and site-specific recombination [79].…”

Section: New Directions Of Soybean Genetic Engineering Skills and Vementioning

confidence: 99%

An Overview of Genetic Transformation of Soybean

Lee¹,

Park²,

Zhang³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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“…Thus, this strategy may not be compatible with vegetatively propagated plants. Although using transposition vectors results in a lower transformation efficiency, a low frequency of single-copy integration, and a low number of backbone-free transformation events compared to conventional T-DNA, a higher expression level of transgenes inserted by transposition has been observed (Yan and Rommens, 2007).…”

Section: Binary and Alternative Vectorsmentioning

confidence: 99%

Insights Into Genetic and Molecular Elements for Transgenic Crop Development

et al. 2020

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Climate change and the exploration of new areas of cultivation have impacted the yields of several economically important crops worldwide. Both conventional plant breeding based on planned crosses between parents with specific traits and genetic engineering to develop new biotechnological tools (NBTs) have allowed the development of elite cultivars with new features of agronomic interest. The use of these NBTs in the search for agricultural solutions has gained prominence in recent years due to their rapid generation of elite cultivars that meet the needs of crop producers, and the efficiency of these NBTs is closely related to the optimization or best use of their elements. Currently, several genetic engineering techniques are used in synthetic biotechnology to successfully improve desirable traits or remove undesirable traits in crops. However, the features, drawbacks, and advantages of each technique are still not well understood, and thus, these methods have not been fully exploited. Here, we provide a brief overview of the plant genetic engineering platforms that have been used for proof of concept and agronomic trait improvement, review the major elements and processes of synthetic biotechnology, and, finally, present the major NBTs used to improve agronomic traits in socioeconomically important crops.

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Transposition-Based Plant Transformation

Cited by 8 publications

References 44 publications

Cytokinin vectors mediate marker-free and backbone-free plant transformation

Cytokinin vectors mediate marker-free and backbone-free plant transformation

An Overview of Genetic Transformation of Soybean

Insights Into Genetic and Molecular Elements for Transgenic Crop Development

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