An Overview of Genetic Transformation of Soybean

Lee, Hyeyoung; Park, So‐Yon; Zhang, Zhanyuan J.

doi:10.5772/51076

Cited by 17 publications

(12 citation statements)

References 99 publications

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“…Under these protocol conditions, Mexican varieties such as Huasteca-100, Nainari and Suaqui-86 had a better regeneration capacity than Jack in terms of the number of shoots produced from one explant. In previous studies, Jack has been found to induce highly embryogenic responses (Lee et al, 2013), which makes it optimal for somatic embryogenesis regeneration approaches. However, our results have shown that the Jack genotype was not highly proliferative in a direct regeneration approach using the cotyledonary node as explant, which is in agreement with recently reported results (Raza et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection

2018

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Background and Aims: Soybean regeneration and transformation are considered highly genotype-specific; however, little is known about desirable seed traits that could be useful indicators of their regeneration and transformation capacities. In the present study, eight commercially available Mexican soybean varieties, as well as the Jack genotype as a control, were phenotypically characterized to determine the seed traits that are important factors for their in vitro performance and susceptibility to Agrobacterium tumefaciens infection.Methods: Grain dimensions, seed weight, moisture, oil, protein, ash, carbohydrate content and macro and micro elements (Mg, K, Ca, P, Na, Mn, Fe, Cu and Zn) were measured and correlated with the corresponding direct shoot organogenesis capability and Agrobacterium infection susceptibility using the cotyledonary node as explant.Key results: Notably, the ash content was the only important factor that inversely correlated with the capacity for shoot organogenesis, whereas carbohydrate and phosphorus content were positively correlated with susceptibility to Agrobacterium infection. The best response in terms of multiple shoot formation and Agrobacterium transient transformation was observed with the soybean varieties Huasteca-100, Nainari and Suaqui-86, which have lower ash content and a higher carbohydrate and phosphorus content.Conclusions: In most reported studies, seed phenotypic traits have been overlooked as factors that influence their regeneration and transformation potential. In the present study, we present evidence of associations between some specific seed traits and regeneration and transient transformation of soybean.

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Section: Discussionmentioning

confidence: 99%

Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection

2018

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“…Soybean plants overexpressing GmNHX5 were generated using Agrobacterium tumefaciens - mediated cotyledonary node transformation following a previously published protocol ( Paz et al, 2004 ; Lee et al, 2013 ), and the soybean cultivar Mustang was used as the transformation recipient. Agrobacterium tumefaciens strain EHA105 carrying plasmid for GmNHX5 -overexpression was used for soybean transformation.…”

Section: Methodsmentioning

confidence: 99%

A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

Sun

Wang

et al. 2021

Front. Plant Sci.

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Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene GmNHX5 and revealed its functional mechanism involved in the salt tolerance process in soybean. GmNHX5 responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of GmNHX5 improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when GmNHX5 was knockout by CRISPR/Cas9. Soybean seedlings overexpressing GmNHX5 also showed an increased expression of GmSOS1, GmSKOR, and GmHKT1, higher K+/Na+ ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.

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“…Soybean can be routinely transformed using either Agrobacterium (preferably with cotyledonary node explants) or particle bombardment (preferably with meristems), but the most efficient transformation protocols are not genotype-independent [199,200,201]. A variety of other explant tissues have been tested to identify the best targets for transformation and regeneration [105,202].…”

Section: Regenerationmentioning

confidence: 99%

Genome Editing in Agriculture: Technical and Practical Considerations

Jansing

Schiermeyer

Schillberg

et al. 2019

IJMS

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The advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture.

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An Overview of Genetic Transformation of Soybean

Cited by 17 publications

References 99 publications

Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection

Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection

A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

Genome Editing in Agriculture: Technical and Practical Considerations

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