Transportation of Helicobacter pylori cultures by optimal systems

Xia, Hua-Xiang; Keane, C. T.; Chen, Jingjing; Zhang, Jianzhong; Walsh, Evelyn J.; Moran, Anthony P.; Hua, Jian; Mégraud, Françis; O’Moráin, Colm

doi:10.1128/jcm.32.12.3075-3077.1994

Cited by 16 publications

(13 citation statements)

References 11 publications

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“…on solid and in liquid media of the same composition although Colony count (log 10 cfu ml −1 ) of H. pylori some variations in growth between the strains occurred (data strains after incubation in: ------------------------------------ yield growth of any micro-organisms. --------------------------------------------- -------------------------------------------------- -------------------------------------------------- ---------------------------------------------------- Morgan et al 1987;Secker et al 1991;Shahamat et al 1991;Oliveri et al 1993;Xia et al 1993a;Morshed et al 1994) and on the influence of different solid media compositions on growth (Goodwin et al 1985;Buck and Smith 1987;Hazell et al 1989;Cellini et al 1992;Oliveri et al 1993) or on the survival of H. pylori in different transport systems (Soltesz et al 1992;Xia et al 1993bXia et al , 1994. To date, no intensive study has examined the influence of media composition and supplementation on the production of LPS by H. pylori.…”

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confidence: 99%

Influence of medium composition on the growth and antigen expression of Helicobacter pylori

Walsh

Moran

1997

J Appl Microbiol

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An evaluation of the ability of various solid and liquid media to support both growth and antigen expression, particularly lipopolysaccharide (LPS) expression, by Helicobacterpylori culture collection strains and clinical isolates was performed. Liquid‐based basal media (brain heart infusion, Brucella broth, Mueller–Hinton broth and tryptone soya broth) supported the growth of strains, whereas solid basal media of the same formulation did not support growth. Optimal growth of all strains was obtained on solid and in liquid media containing blood. Supplemented solid media containing supplements other than blood supported growth but only to a small extent. In liquid media excluding blood, serum supplements enhanced growth and horse serum was found to be superior to fetal calf serum. In general, β‐cyclodextrin did not increase growth. Mueller–Hinton broth or tryptone soya broth containing horse serum and a nitrogen source such as yeast extract or proteose peptone no. 3 were found to give optimal growth of H. pylori in a blood‐free environment. Strains after cultivation in liquid media, irrespective of composition, maintained production of high‐molecular weight (mol. wt) LPS with an O side chain independent of medium composition, whereas subculturing on solid media resulted in production of low‐mol. wt LPS. Expression of proteins differed in liquid and on solid media, particularly proteins of 57 and 60 kDa, but qualitatively no differences were observed upon supplementation of basal media.

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confidence: 99%

Influence of medium composition on the growth and antigen expression of Helicobacter pylori

Walsh

Moran

1997

J Appl Microbiol

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“…However, there is still no universal medium used to sustain the growth of the organism, and no medium has been identified that provides significant growth advantages in vitro. Plating media that have commonly been used to cultivate H. pylori contain either Columbia agar, brain heart infusion agar (BHIA) or charcoal base agar, supplemented with either whole, laked or chocolated blood or serum (Goodwin et al 1985;Dent and McNulty 1988;Xia et al 1994;Henriksen et al 1995;Benaïssa et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Growth of Helicobacter pylori in various liquid and plating media

Stevenson

Castillo

Lucia

et al. 2000

Lett Appl Microbiol

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The objectives of this research were to compare commonly used liquid and plating media to elucidate whether one medium provided superior growth of Helicobacter pylori in vitro. The liquid media compared were Mueller‐Hinton broth, brain heart infusion broth and H. pylori special peptone broth, formulated in this laboratory. No significant differences in growth rates were noted and shaking during the incubation of broths was not essential for good growth. The plating media compared included Columbia agar, Mueller‐Hinton agar, modified Glupczynski's Brussels campylobacter charcoal agar, Johnson‐Murano agar and H. pylori special peptone agar (HPSPA). None of the non‐specific plating media that have been used historically to culture H. pylori exhibited any particular advantage. However, HPSPA provided an obvious advantage in colony size. Helicobacter pylori special peptone agar enhances the cultivation of H. pylori and could improve the recovery of the bacterium from clinical samples in vitro.

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“…Therefore SATFM can be used as transport medium for H. pylori, provided that storage time preferentially stays below 5 days. Several other transport media have been described for H. pylori (Wang et al, 1980;Owen et al, 1988;Xia et al, 1994). Their success is probably due to the fact that they contain rich sources of nutrients using blood or serum and/ or other animal derived components.…”

Section: Satfm Can Be Used As Transport Medium For H Pylorimentioning

confidence: 99%

“…The important human pathogen Helicobacter pylori is a fastidious organism, which requires complex media and microaerobic conditions for survival and growth . Therefore, peptic digest of animal tissue, beef extract, bovine serum albumine, blood or serum are commonly used in growth, storage and in transport media for H. pylori (Shahamat et al, 1991;Olivieri et al, 1993;Xia et al, 1994). Special care must be taken in using these materials in transport or growth media used for production of H. pylori lysates for immunization (Suzuki et al, 2004), as their use could potentially lead to the unintentional spread of infectious agents.…”

Section: Introductionmentioning

confidence: 99%

“…A consequence of the importance of H. pylori as a human pathogen is that H. pylori strains are being shipped between various laboratories at different geographical regions. Different protocols have been developed for the storage and transport of H. pylori strains (Wang et al, 1980;Owen et al, 1988;Soltesz et al, 1992;Xia et al, 1994). It is possible to ship the bacteria on dry ice, but this is expensive, and not always feasible.…”

Section: Introductionmentioning

confidence: 99%

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Serum- and animal tissue-free medium for transport and growth ofHelicobacter pylori

Dierikx

Martodihardjo²,

Kuipers

et al. 2007

FEMS Immunol Med Microbiol

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The important human gastric pathogen Helicobacter pylori is the subject of many studies, and as a consequence it is frequently being transported between national and international laboratories. Unfortunately, common bacterial growth and transport media contain serum- and animal tissue-derived materials, which carry the risk of spreading infectious diseases. We have therefore developed a growth and transport medium for H. pylori, designated 'Serum- and Animal Tissue-Free Medium' (SATFM), which does not contain serum- or animal tissue-derived components. SATFM supported growth of H. pylori isolates to similar levels as obtained with serum-supplemented Brucella medium, and SATFM with 0.5% agar supported transport and storage of H. pylori strains, as 4/4 reference strains and 11/11 clinical isolates survived for at least 3 days at room temperature in SATFM, with some strains (2/15) even surviving for up to 7 days. In conclusion, SATFM can be used both as transport and growth medium for H. pylori. The formulation of SATFM may allow its use in international transport of H. pylori, and may also allow certified use in immunization studies requiring growth of H. pylori and other bacterial pathogens.

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Transportation of Helicobacter pylori cultures by optimal systems

Cited by 16 publications

References 11 publications

Influence of medium composition on the growth and antigen expression of Helicobacter pylori

Influence of medium composition on the growth and antigen expression of Helicobacter pylori

Growth of Helicobacter pylori in various liquid and plating media

Serum- and animal tissue-free medium for transport and growth ofHelicobacter pylori

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