Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

Marques, Marcelo Rodrigues; Cerda, Álvaro; Fontanari, Gustavo Guadagnucci; Pimenta, Daniel C.; Soares-Freitas, Rosana Manólio; Hirata, Mário Hiroyuki; Hirata, Rosário Dominguez Crespo; Arêas, José Alfredo Gomes

doi:10.1016/j.foodres.2018.01.031

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…Cardiovascular diseases have become a major cause of mortality and morbidity in both the developed and developing world, and dyslipidemia is considered to be a main reason for the present condition . Cholesterol metabolism is a complex scenario and, even after years of research, there is still a gap in the clear understanding of this process . So far, treatment of hypercholesterolemia mainly focuses on modulating the intestinal absorption of fat and the endogenous biosynthesis of cholesterol …”

Section: Functional Propertiesmentioning

confidence: 99%

“…In addition, the molecular mechanisms involved in the reduction of hyperlipidemia by compounds present in cowpea are not yet completely understood. A recent study reported that cowpea peptides interfere with the intestinal absorption of cholesterol by inhibiting the expression of NPC1L1 and reducing cholesterol synthesis via a lowered expression of the transcription factor SREBP2 (consequently HMGCR and LDLR) . However, there is still a huge gap to be filled in the literature regarding the actual mechanism by which cowpea components exert their hypocholesterolemic activity.…”

Section: Functional Propertiesmentioning

confidence: 99%

“…41,115,148 Cholesterol metabolism is a complex scenario and, even after years of research, there is still a gap in the clear understanding of this process. 116,148 So far, treatment of hypercholesterolemia mainly focuses on modulating the intestinal absorption of fat and the endogenous biosynthesis of cholesterol. 41,148 Observational studies throughout the world indicate that increased consumption of legumes is a promising route for reducing cardiovascular disease risk factors, such as elevated blood cholesterol, blood glucose, blood pressure and body weight.…”

Section: Hypocholesterolemic Activitymentioning

confidence: 99%

“…A recent study reported that cowpea peptides interfere with the intestinal absorption of cholesterol by inhibiting the expression of NPC1L1 and reducing cholesterol synthesis via a lowered expression of the transcription factor SREBP2 (consequently HMGCR and LDLR). 116 However, there is still a huge gap to be filled in the literature regarding the actual mechanism by which cowpea components exert their hypocholesterolemic activity. Thus, further studies should be carried out to enable a clear view with respect to the mechanistic approach of cowpea on hypocholesterolemia.…”

Section: Hypocholesterolemic Activitymentioning

confidence: 99%

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Cowpea: an overview on its nutritional facts and health benefits

et al. 2018

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Cowpea (Vigna unguiculata) is a legume consumed as a high-quality plant protein source in many parts of the world. High protein and carbohydrate contents with a relatively low fat content and a complementary amino acid pattern to that of cereal grains make cowpea an important nutritional food in the human diet. Cowpea has gained more attention recently from consumers and researchers worldwide as a result of its exerted health beneficial properties, including anti-diabetic, anti-cancer, anti-hyperlipidemic, anti-inflammatory and anti-hypertensive properties. Among the mechanisms that have been proposed in the prevention of chronic diseases, the most proven are attributed to the presence of compounds such as soluble and insoluble dietary fiber, phytochemicals, and proteins and peptides in cowpea. However, studies on the anti-cancer and anti-inflammatory properties of cowpea have produced conflicting results. Some studies support a protective effect of cowpea on the progression of cancer and inflammation, whereas others did not reveal any. Because there are only a few studies addressing health-related effects of cowpea consumption, further studies in this area are suggested. In addition, despite the reported favorable effects of cowpea on diabetes, hyperlipidemia and hypertension, a long-term epidemiological study investigating the association between cowpea consumption and diabetes, cardiovascular disease and cancer is also recommended. © 2018 Society of Chemical Industry.

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Section: Functional Propertiesmentioning

confidence: 99%

Section: Functional Propertiesmentioning

confidence: 99%

Section: Hypocholesterolemic Activitymentioning

confidence: 99%

Section: Hypocholesterolemic Activitymentioning

confidence: 99%

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Cowpea: an overview on its nutritional facts and health benefits

et al. 2018

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“…Five bands with a molecular weight of 35,23,20,12, and 11 kDa were separated from the axseed protein (Fig. 2A).…”

Section: Protein Characterization and Ippf-speci C Antibody Western Blot Reactionmentioning

confidence: 99%

Flaxseed-Derived Peptide, IPPF, Inhibits Intestinal Cholesterol Absorption and Hepatic Cholesterol Synthesis in Caco-2 and HepG2 Cells

Bao

Yuan

et al. 2021

Preprint

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Background：Flaxseed peptide (FPs) showed serum cholesterol-lowering activity in SD rats fed a high-cholesterol diet, but the cholesterol-lowering amino acid sequences and mechanism of FPs were still unclear. Methods: FPs were separated via ultrafiltration, and the amino acid sequences of the selected fractions were determined via high-performance liquid chromatography- Electrospray Ionisation - Orbitrap- Mass spectrometry (HPLC-ESI-Orbitrap MS). These peptides then were synthesized by solid-phase synthesis (SPPS). IPPF with the highest CMSR was determined to exist in flaxseed protein by specific antibodies. The effects of IPPF on intestinal cholesterol absorption and hepatic cholesterol metabolism were investigated in Caco-2 cells and HepG2 cells.Results：1 kDa FP5 fraction had the highest cholesterol micelle solubility inhibition rate (CMSR) 72.39% compared with the other ultrafiltration fractions. Then Eleven peptides were identified from FP5. Ile-Pro-Pro-Phe (IPPF), with the highest CMSR 93.47%, was selected to research the cholesterol-lowering mechanism in Caco-2 and HepG2 cells. IPPF significantly reduces the amount of cholesterol transported in Caco2 cells and the amount of total cholesterol in HepG2 cells. IPPF significantly modulated the protein levels of NCP1L1 and ABCG5/8 in Caco2 cells and significantly reduced the mRNA levels of Srebp-2 and Hmgcr in HepG2 cells. Conclusion: IPPF inhibits cholesterol intestinal absorption through modulating the expression of cholesterol transporters in Caco-2 cells and reduces hepatic cholesterol synthesis through inhibiting the SREBP2-regulated mevalonate (HMGCR) pathway in HepG2 cells. IPPF is a new food-derived inhibitor of intestinal cholesterol absorption and hepatic cholesterol synthesis without side effects and provides a nutritional therapy component for hypercholesterolemia.

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Gastrointestinal digestion and absorption characterization in vitro of zinc‐chelating hydrolysate from scallop adductor (Patinopecten yessoensis)

et al. 2021

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BACKGROUD: Zinc (Zn) is an essential catalytic element in the human health system but its absorption in the intestinal system can be strongly affected by gastrointestinal (GI) digestion. In this study, the food-derived potential Zn carrier, scallop adductor hydrolysates (SAHs), was produced and characterized. RESULTS: During temporary storage at 4 °C, SAH decreased in Zn-chelating capacity in the aqueous phase, whereas the SAH-Zn complex exhibited high stability. Moreover, the secondary structure of SAH had no significant alteration. Zn morphologically altered the surface structures of SAH, which was involving in carboxyl group of SAH. Results of in vitro GI digestion suggested that the SAH-Zn maintained good stability in GI system and only proportion of high molecular weight cleaved. In addition, SAH could successfully carry and transport Zn while the fluorescence staining revealed free Zn accumulation inside the tissue. Finally, three representative absorbed peptides (around 600 Da) were identified and synthesized. Three synthetic peptides exhibit higher Zn-chelating capacity than SAH and could also successfully transported through the intestine.CONCLUSION: This study provided a theoretical basis for the investigation of digestion and absorption of marine animalderived peptides as Zn carriers.

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Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

Cited by 23 publications

References 30 publications

Cowpea: an overview on its nutritional facts and health benefits

Cowpea: an overview on its nutritional facts and health benefits

Flaxseed-Derived Peptide, IPPF, Inhibits Intestinal Cholesterol Absorption and Hepatic Cholesterol Synthesis in Caco-2 and HepG2 Cells

Gastrointestinal digestion and absorption characterization in vitro of zinc‐chelating hydrolysate from scallop adductor (Patinopecten yessoensis)

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