Gastrointestinal digestion and absorption characterization in vitro of zinc‐chelating hydrolysate from scallop adductor (Patinopecten yessoensis)

Abstract

BACKGROUD: Zinc (Zn) is an essential catalytic element in the human health system but its absorption in the intestinal system can be strongly affected by gastrointestinal (GI) digestion. In this study, the food-derived potential Zn carrier, scallop adductor hydrolysates (SAHs), was produced and characterized. RESULTS: During temporary storage at 4 °C, SAH decreased in Zn-chelating capacity in the aqueous phase, whereas the SAH-Zn complex exhibited high stability. Moreover, the secondary structure of SAH had no significant alteration. Zn morphologically altered the surface structures of SAH, which was involving in carboxyl group of SAH. Results of in vitro GI digestion suggested that the SAH-Zn maintained good stability in GI system and only proportion of high molecular weight cleaved. In addition, SAH could successfully carry and transport Zn while the fluorescence staining revealed free Zn accumulation inside the tissue. Finally, three representative absorbed peptides (around 600 Da) were identified and synthesized. Three synthetic peptides exhibit higher Zn-chelating capacity than SAH and could also successfully transported through the intestine.CONCLUSION: This study provided a theoretical basis for the investigation of digestion and absorption of marine animalderived peptides as Zn carriers.

Characterisation of zinc‐chelating peptides derived from scallop (Patinopecten yessoensis) mantle and their intestinal transport in everted rat sacs

“…Based on the results, the existence of signals of zinc was clearly detected from the SMH‐Zn complex, suggesting that zinc successfully chelated SMH. Compared with our previous results, we also used elemental signatures tests of SEM to scan both food‐derived hydrolysates and synthetic peptides and the consistent results were obtained, reflected by similar contents of zinc (2.83% (Sun et al ., 2021a) and 6.88% (Wang et al ., 2022)). Therefore, in zinc‐chelated sea food‐derived hydrolysates or peptides, the proportion of zinc content might be no more than 10%, meaning the molecular ratio of peptides and zinc during chelation might be no more than 1:2 that was consistent with our previous results (Liu et al ., 2019a).…”

“…In this study, we chose two groups of temperature (30–90 °C) and incubation time (10–50 min) into consideration of selection. Based on our results, we found that incubation temperature was not significantly involved in influencing the zinc‐chelating ability as well as our previous study (Sun et al ., 2021a) even though the other reposts discovered that proper heat would provide the energy (Sun et al ., 2021c) and improved the chelation between food‐derived peptides and metal (Zhang et al ., 2021). On the other hand, we also discovered that SMH could maintain relatively high zinc‐chelating ability in the high temperature of 80–90 °C with impressive heat stability.…”

Preliminary study on toxicological mechanism of golden cuttlefish (Sepia esculenta) larvae exposed to cd

“…Digestion and absorption are essential processes to maintain the life activities of organisms [ 74 ]. Disorder of this process will significantly reduce the efficiency of energy acquisition and inhibit the absorption and transport of nutrients [ 75 , 76 ]. In this study, protein digestion and absorption signaling pathway and carbohydrate digestion and absorption signaling pathway were enriched, and most DEGs enriched in pathways were down-regulated.…”