Transport-in-vitro fertilization/intracellular sperm injection: a prospective randomized study

Coetsier, Tom; Verhoeff, Arie; Sutter, P. De; Roest, Jan; Dhont, Marc

doi:10.1093/humrep/12.8.1654

Cited by 20 publications

(17 citation statements)

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“…Recently, several methods for embryo transportation have been reported. Human oocytes and embryos have been transported between two IVF centers using portable but heavy CO 2 incubators with no detrimental effects on fertilization or pregnancy rates [34], [35]. Chen et al also reported that mouse oocytes could be transported to another facility using an incubator without a CO 2 supply, but the incubation time was only 5 h [36].…”

Section: Discussionmentioning

confidence: 99%

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

Itoi¹,

Tokoro²,

Terashita

et al. 2012

PLoS ONE

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To culture preimplantation embryos in vitro, water-jacketed CO2 incubators are used widely for maintaining an optimal culture environment in terms of gas phase, temperature and humidity. We investigated the possibility of mouse embryo culture in a plastic bag kept at 37°C. Zygotes derived from in vitro fertilization or collected from naturally mated B6D2F1 female mice were put in a drop of medium on a plastic culture dish and then placed in a commercially available plastic bag. When these were placed in an oven under air at 37°C for 96 h, the rate of blastocyst development and the cell numbers of embryos decreased. However, when the concentration of O2 was reduced to 5% using a deoxidizing agent and a small oxygen meter, most zygotes developed into blastocysts. These blastocysts were judged normal according to their cell number, Oct3/4 and Cdx2 gene expression levels, the apoptosis rate and the potential for full-term development after embryo transfer to pseudopregnant recipients. Furthermore, using this system, normal offspring were obtained simply by keeping the bag on a warming plate. This culture method was applied successfully to both hybrid and inbred strains. In addition, because the developing embryos could be observed through the transparent wall of the bag, it was possible to capture time-lapse images of live embryos until the blastocyst stage without needing an expensive microscope-based incubation chamber. These results suggest that mouse zygotes are more resilient to their environment than generally believed. This method might prove useful in economical culture systems or for the international shipment of embryos.

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Section: Discussionmentioning

confidence: 99%

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

Itoi¹,

Tokoro²,

Terashita

et al. 2012

PLoS ONE

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“…PREVIOUS TRANSPORT ART studies have already indicated no differences in rates of fertilization, cleavage, transfer or pregnancy with conventional IVF and ICSI using the transport oocyte method and performed entirely at the ART center. Delay as a result of transportation in commencement of culture does not reduce the pregnancy rate, reduce the ability during ICSI of the oocyte to endure sperm injection, or subsequent embryo development and ability to implant 7–16 . We have also achieved favorable results with our round trip method, where transported fresh embryos are implanted at satellite clinics 6,17,18 .…”

Section: Discussionmentioning

confidence: 88%

“…Delay as a result of transportation in commencement of culture does not reduce the pregnancy rate, reduce the ability during ICSI of the oocyte to endure sperm injection, or subsequent embryo development and ability to implant. [7][8][9][10][11][12][13][14][15][16] We have also achieved favorable results with our round trip method, where transported fresh embryos are implanted at satellite clinics. 6,17,18 These results suggest that transport does not affect the viability of oocytes or fresh embryos.…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation of frozen/thawed embryo transfer following transport of oocytes and embryos

Takanashi

2004

Reprod Medicine & Biology

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Background and Aims:We evaluated the efficacy of the transport oocyte/embryo frozen/thawed embryo transfer method, in which oocytes or embryos were transported from satellite clinics to the main assisted reproductive technology (ART) center, and surplus embryos were placed in cryopreservation. Methods:We evaluated 41 cycles in 34 patients in the transport oocyte group (TO group). In the TO group the oocytes were collected at the satellite clinics, transported to the main ART center and underwent in vitro fertilization or intracytoplasmic sperm injection. Surplus embryos were used for frozen/thawed embryo transfer. We also evaluated 17 cycles in 10 patients in the transport embryo group (TE group), where surplus embryos were transported to the main ART center and used for frozen/thawed embryo transfer; and 189 cycles in 134 patients in the center group (C group), where surplus embryos collected at the same time at the main ART center were used for frozen/thawed embryo transfer. Oocytes were transported from satellite clinics in HEPES buffered human tubal fluid (HTF) culture medium, and embryos in 30% synthetic serum substitute + HEPES buffered HTF, using a portable incubator we devised. Results:The proportions of undamaged embryos after freeze/ thawing were 47% for the C group, 46% for the TO group, and 46% for the TE group. The numbers of embryos transferred were 2.0 ± 0.7 for the C group, 2.0 ± 0.6 for the TO group, and 2.2 ± 0.4 for the TE group. The rate of embryo transfer was 63% for the C group, 68% for the TO group, and 76% for the TE group. Pregnancy rates per patient were 16% for the C group, 24% for the TO group, and 40% for the TE group. The embryo survival rates (number of embryos with ≥50% viable blastomeres/total number of embryos) were 55% for the C group, 60% for the TO group, and 54% for the TE group. No significant differences were seen between the C group and either the TO or TE groups in any of these parameters. Conclusions:

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“…In the present study, however, the same method of embryonic culture was used in the main ART center and satellites, and the only point of difference was oocyte transportation. It is generally believed that during transportation the follicular liquid does not disturb the oocyte 4 and others have reported that after oocyte collection the follicular liquid is transported unaltered, without identification or washing of the oocytes 4,7,18,19 . The satellites of our ART facility also do not identify the oocytes using stereoscopic microscopy, and do not transfer the cumulus–oocyte complex to a transportation medium, in order to prevent loss of the oocytes from poor procedures and to shorten the transportation time.…”

Section: Discussionmentioning

confidence: 99%

“…P REVIOUS REPORTS OF transport assisted reproductive technology (ART) suggest that transportation per se does not adversely affect the oocytes. [1][2][3][4][5][6][7][8] During the past 6 years, we have used transport ART in more than 250 patients, collecting oocytes from more than 350 cycles. Our transport ART network comprises the center facility (at the Department of Obstetrics and Gynecology, Omori Hospital attached to the Toho University School of Medicine, Tokyo, Japan) and satellites, including three facilities that use the round-trip method in which oocytes and embryos are transported between the main ART center and the satellite facilities, 9,10 11 facilities that use the one-way method for oocytes, one facility that transports embryos to the center for cryopreservation, 11 and three facilities where the ART treatment modality is selected on an individual basis ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Effect of oocyte transportation time on the clinical results of transport in vitro fertilization/intracytoplasmic sperm injection‐embryo transfer

Takanashi

Abe

Shibui

et al. 2004

Reprod Medicine & Biology

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In transport assisted reproductive technology (ART), the time taken to transport oocytes to the main center differs greatly among the satellite facilities and may influence the clinical results. For the conventional fertilization (IVF) groups in which oocytes were collected at the satellite facilities and transported to the main ART center for insemination and embryo transfer, there were 29 cycles in 27 patients with a transportation time within 60 min (short time transport IVF (ST-IVF)), 78 cycles in 62 patients with a time between 60 and 120 min (long time transport IVF (LT-IVF)), and there were 141 cycles in 110 patients at the main ART center (center IVF (C-IVF)). For the intracytoplasmic sperm injection (ICSI) group, there were 65 ST-ICSI cycles in 42 patients, 146 LT-ICSI cycles in 97 patients, and 326 cycles in 238 patients at the main ART center (C-ICSI). The morphologically favorable embryo rate was lower in the ST-ICSI group (33.8%, < 0.05) than in the C-ICSI group (38.1%), and the morphologically poor embryo rate in the LT-IVF group (38.6%, < 0.0001) was higher than in the C-IVF group (26.7%). The rate of embryo transfers resulting in pregnancies was 16.7% in the ST-ICSI group ( < 0.01) and 17.3% in the LT-ICSI group ( < 0.001), both less than that of 35.2% for the C-ICSI group. To improve both the morphologically favorable embryo rate and the pregnancy rate in transport ART, it is essential to improve the total quality control at the satellite facilities. (Reprod Med Biol 2004;: 123-131).

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Transport-in-vitro fertilization/intracellular sperm injection: a prospective randomized study

Cited by 20 publications

References 0 publications

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

Clinical evaluation of frozen/thawed embryo transfer following transport of oocytes and embryos

Effect of oocyte transportation time on the clinical results of transport in vitro fertilization/intracytoplasmic sperm injection‐embryo transfer

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