Effect of oocyte transportation time on the clinical results of transport in vitro fertilization/intracytoplasmic sperm injection‐embryo transfer

Abstract

In transport assisted reproductive technology (ART), the time taken to transport oocytes to the main center differs greatly among the satellite facilities and may influence the clinical results. For the conventional fertilization (IVF) groups in which oocytes were collected at the satellite facilities and transported to the main ART center for insemination and embryo transfer, there were 29 cycles in 27 patients with a transportation time within 60 min (short time transport IVF (ST-IVF)), 78 cycles in 62 patients with a time between 60 and 120 min (long time transport IVF (LT-IVF)), and there were 141 cycles in 110 patients at the main ART center (center IVF (C-IVF)). For the intracytoplasmic sperm injection (ICSI) group, there were 65 ST-ICSI cycles in 42 patients, 146 LT-ICSI cycles in 97 patients, and 326 cycles in 238 patients at the main ART center (C-ICSI). The morphologically favorable embryo rate was lower in the ST-ICSI group (33.8%, < 0.05) than in the C-ICSI group (38.1%), and the morphologically poor embryo rate in the LT-IVF group (38.6%, < 0.0001) was higher than in the C-IVF group (26.7%). The rate of embryo transfers resulting in pregnancies was 16.7% in the ST-ICSI group ( < 0.01) and 17.3% in the LT-ICSI group ( < 0.001), both less than that of 35.2% for the C-ICSI group. To improve both the morphologically favorable embryo rate and the pregnancy rate in transport ART, it is essential to improve the total quality control at the satellite facilities. (Reprod Med Biol 2004;: 123-131).

Transport IVF-ICSI: Results of a 25-year experience

“…Transport IVF is used in other countries in Europe ( Kingsland et al ., 1992 ; De Sutter et al ., 1996 ; Qureshi et al ., 1997 ), Canada ( Buckett et al ., 1999 ) and Japan ( Takanashi et al ., 2004 ). Fresh embryo transport is also available in the USA ( Langley et al ., 2001 ), Ukraine ( Levron et al , 2014 ), and Japan ( Takanashi et al, 2005 ), with satisfying results.…”

Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

“…8 Transported non-human oocytes are also regarded as valuable translational research materials for human oocyte studies, because it is difficult to obtain sufficiently large numbers of human oocytes. 10 There have also been reports on the transportation of human follicular fluid (HFF) [11][12][13] and ovaries. [14][15][16][17] Prior to two decades ago, only a few institutions performed intra-cytoplasmic sperm injection (ICSI), and therefore, HFF aspirated from stimulated ovaries was transported by car and airplane from satellite clinics to in vitro fertilization centers [11][12][13] ; this transportation occurred at distances of 90-500 km at temperatures of 37°C with no 5% CO 2 chamber.…”

“…10 There have also been reports on the transportation of human follicular fluid (HFF) [11][12][13] and ovaries. [14][15][16][17] Prior to two decades ago, only a few institutions performed intra-cytoplasmic sperm injection (ICSI), and therefore, HFF aspirated from stimulated ovaries was transported by car and airplane from satellite clinics to in vitro fertilization centers [11][12][13] ; this transportation occurred at distances of 90-500 km at temperatures of 37°C with no 5% CO 2 chamber. [11][12][13] Transportation of resected human ovaries has also been examined, as it is an important fertility preservation (FP) technique in the field of oncofertility for patients with malignant tumors such as breast and blood cancers.…”

“…[14][15][16][17] Prior to two decades ago, only a few institutions performed intra-cytoplasmic sperm injection (ICSI), and therefore, HFF aspirated from stimulated ovaries was transported by car and airplane from satellite clinics to in vitro fertilization centers [11][12][13] ; this transportation occurred at distances of 90-500 km at temperatures of 37°C with no 5% CO 2 chamber. [11][12][13] Transportation of resected human ovaries has also been examined, as it is an important fertility preservation (FP) technique in the field of oncofertility for patients with malignant tumors such as breast and blood cancers. [14][15][16][17] Researchers have investigated a variety of outcomes of in vitro maturation (IVM) and artificial reproductive technology (ART) after ovarian transportation for clinical purposes.…”

“…In our previous report, the numbers of collected oocytes and IVM outcomes were investigated in eight patients with EMA. There have been several reports of transportation of HFF, but none have examined outcomes of IVM of human oocytes collected from transported HFF aspirated from resected ovaries. Currently, HFF transportation is not necessary for ICSI because this technique has become widespread, and is performed even in satellite clinics.…”