Transplanted adipose-derived stem cells can be short-lived yet accelerate healing of acid-burn skin wounds: a multimodal imaging study

Abstract

The incidence of accidental and intentional acid skin burns is rising. Current treatment strategies are mostly inadequate, leaving victims disfigured and without treatment options. Here, we have shown that transplantation of adipose-derived stem cells (ASCs) accelerates the process of acid burn wound-healing. Pre-conditioning of ASCs using ascorbic acid (AA) or hypoxic conditions provided additional benefit. While the wounds were ultimately healed in all mice, histological analysis revealed that, in non-transplanted animals, the number of hair follicles was reduced. Bioluminescent imaging (BLI) of transplanted ASCs revealed a gradual loss of transplanted cells, with a similar rate of cell death for each treatment group. The signal of fluorinated cells detected by a clinically applicable 19F MRI method correlated with the BLI findings, which points to 19F MRI as a reliable method with which to track ASCs after transplantation to skin wounds. No difference in therapeutic effect or cell survival was observed between labeled and non-labeled cells. We conclude that, despite being short-lived, transplanted ASCs can accelerate wound-healing and reduce hair loss in acid-burn skin injury. The fluorine nanoemulsion is a clinically applicable cell label capable of reporting on the survival of transplanted cells.

Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels

“…7 Although it was initially thought that the therapeutic effects of MSCs depend on their capabilities for cell replacement, many studies have demonstrated that paracrine activity is the predominant mechanism by which MSCs participate in tissue repair. [8][9][10] It was shown that by the secretion of different bioactive molecules, MSCs may support endogenous stem cell proliferation, Dovepress Dovepress 1654 Dabrowska et al reduce apoptosis, promote angiogenesis, and modulate immune reactions. [11][12][13][14][15] Previous studies have focused on characterizing MSC-produced soluble factors, ie, cytokines, chemokines, and growth factors, but it has been recently documented that MSCs release also large numbers of extracellular vesicles (EVs).…”

Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats

“…Preparation of allograft ADS. The ADS were obtained from adipose tissue within the abdominal region of healthy adult rats as described previously 57,58 . About 3 cm 3 of adipose tissue was manually minced and subjected to digestion with 0.1% collagenase type I solution for 25 min at 37 °C, and then centrifuged.…”

“…The cell pellets were suspended in Dulbecco's Modified Eagle's Medium (DMEM) plus 20% fetal bovine serum and then seeded into T-75 flasks that contained DMEM, 20% fetal bovine serum, 100 U/ml penicillin, and 100 µg/ml streptomycin. The ADS were characterized for mesenchymal stem cell markers by flow cytometry, as reported previously 57,58 .…”

Comparable effect of adipose-derived stromal vascular fraction and mesenchymal stem cells for wound healing: An in vivo study

“…It has known that MSCs are often stressed and begin to die after being transplanted, releasing various cytokines and growth factors beneficial for wound healing 15 . In this study, we did not find any significant difference between the SVF-and MSC-treated groups.…”