Transplantation of Simian Mesenchymal Stem Cells to Baboons with Experimentally Induced Myocardial Infarction

Abstract

Culture of mesenchymal stem cells isolated from the bone marrow of primates by their characteristics met the requirements of stem cells. It was shown that transplantation of allogeneic mesenchymal stem cells (2 million cells per 1 kg body weight) immediately after ligation of the left anterior descending coronary artery between the middle and upper thirds led to neovascularization and capillarization of the ischemic myocardium.

Baboon induced pluripotent stem cell generation bypiggyBactransposition of reprogramming factors

“…NHP iPSCs are of relevance for the preclinical evaluation of regenerative therapies (Phillips et al, 2014). This may particularly involve therapies of the central and peripheral nervous system, the eye, the heart, and the vascular system (Stevens et al, 2009;Grow et al, 2016a;Agrba et al, 2016;Längin et al, 2018;Shiba et al, 2016;Emborg et al, 2013;Wang et al, 2015;Chong and Murry, 2014). Marmosets and macaques (rhesus and cynomolgus monkeys) are the most frequently used NHP species in biomedical research, but they differ in some characteristics from humans, including body size (Behr, 2015).…”

“…In terms of body size, immunological characteristics, and other features, the baboon can be an excellent alternative model to mimic human pathology. The similar anatomy, specifically organ size, and immunology of baboons are reflected in their crucial role in (xeno-) transplantation research (Bailey, 2009;Längin et al, 2018). Since different iPSC lines from the same species, even from the same animal, have different properties and may respond differently to external cues, it is expedient to have a representative number of baboon iPSC lines available (Ohi et al, 2011;Carcamo-Orive et al, 2017).…”

“…The olive baboon (Papio anubis) is an Old World monkey with comparable evolutionary distance to humans as the macaques. It shares 92 % of the genome with humans, has a life expectancy of up to 45 years, and very closely resembles human development, anatomy, and physiology (Navara et al, 2018;Bailey, 2009;Rogers and Hixson, 1997). In comparison to macaques and other NHP species except for great apes, baboons have (i) a more similar size and weight in comparison with humans (16.4-21.3 kg for males and 10-15 kg for females) in contrast to rhesus (4-14.1 kg for males and 3-10 kg for females); (ii) they alternate quadrupedal and bipedal locomotion, and (iii) regarding transplantation studies, it is of relevance that their immune system closely resembles the human one, e.g., presenting the same four immunoglobulin G (IgG) subclasses (Grow et al, 2016a;Navara et al, 2018;Varilly and Chandler, 2008;Shearer et al, 1999).…”

“…Furthermore, controlled-breeding colonies exist that have been studied for many years. Overall, baboons are an excellent NHP model for stem-cell-based transplantation studies (Navara et al, 2013;Bailey, 2009;Simerly et al, 2009;Agrba et al, 2016;Cox et al, 2013;Längin et al, 2018).…”