Transplantation of neural stem cells into the spinal cord after injury

Okano, Hideyuki; Ogawa, Yoshifumi; Nakamura, Masaya; Kaneko, Shinjiro; Iwanami, Akio; Toyama, Yoshiaki

doi:10.1016/s1084-9521(03)00011-9

Cited by 143 publications

(102 citation statements)

References 50 publications

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“…Namely, the acute phase of SCI corresponds to the "inflammatory phase" due to the upregulation of inflammatory cytokines, excitatory neurotransmitters and free radicals, and is not suitable for transplantation, whereas in the chronic phase, about 2 weeks or more after injury, the injury enters the stage of glial scar formation, which prevents axonal regeneration. Therefore, the subacute phase of SCI is considered as the optimal time window for NS/PC transplantation in a rat SCI model [10,12,19] (Figure 1). However, we have to realize that the anatomy and functions of the spinal cord are considerably different between rodents and primates.…”

Section: Regenerative Medicine For Sci Using Fetal-derived Ns/pcsmentioning

confidence: 99%

Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells

2012

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Section: Regenerative Medicine For Sci Using Fetal-derived Ns/pcsmentioning

confidence: 99%

Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells

2012

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“…Additionally, the central nervous system (CNS) has limited intrinsic regenerative capacity. Transplantation of neural stem or precursor cells has been used as a reliable therapeutic strategy in restoring tissue damage and regaining lost function in the CNS [1][2][3][4]. Direct cell transplantation, however, suffers several disadvantages such as limited survival upon implant ation and the formation of abnormal cell architecture in vivo [5].…”

Section: Introductionmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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Objective. Nanomaterials, such as carbon nanotubes (CNTs), have been introduced to modify the surface properties of scaffolds, thus enhancing the interaction between the neural cells and biomaterials. In addition to superior electrical conductivity, CNTs can provide nanoscale structures similar to those present in the natural neural environment. The primary objective of this study is to investigate the proliferative capability and differential potential of neural stem cells (NSCs) seeded on a CNT incorporated scaffold. Approach. Amine functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated with a PEGDA polymer to provide enhanced electrical properties as well as nanofeatures on the surface of the scaffold. A stereolithography 3D printer was employed to fabricate a well-dispersed MWCNT-hydrogel composite neural scaffold with a tunable porous structure. 3D printing allows easy fabrication of complex 3D scaffolds with extremely intricate microarchitectures and controlled porosity. Main results. Our results showed that MWCNT-incorporated scaffolds promoted neural stem cell proliferation and early neuronal differentiation when compared to those scaffolds without the MWCNTs. Furthermore, biphasic pulse stimulation with 500 µA current promoted neuronal maturity quantified through protein expression analysis by quantitative polymerase chain reaction. Significance. Results of this study demonstrated that an electroconductive MWCNT scaffold, coupled with electrical stimulation, may have a synergistic effect on promoting neurite outgrowth for therapeutic application in nerve regeneration.

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“…These cells have the ability to self-renew and the multipotent potential to differentiate into neurons, astrocytes and oligodendrocytes. In the name of neuronal cell replacement, transplantation of NSCs has been challenged in a wide range of animal models of diseases and injuries such as Parkinson's disease, Huntington's disease, stroke and spinal cord injury, and functional recovery has often been reported [27,28,35,41,46,63]. As to muscle tissue, muscle stem cells and satellite cells isolated from adult and prenatal tissues [3,21,50,68] and myogenic stem cells contained in the bone marrow [25,64] are considered to be a source of stem cells, and there have been several attempts to ameliorate muscle degeneration by transplantation of these muscle stem cells [25].…”

Section: Introductionmentioning

confidence: 99%

Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

Dezawa

Ishikawa

Hoshino

et al. 2005

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Cell transplantation is a promising strategy for the treatment of neurodegenerative and muscle degenerative diseases. Many kinds of cells, including embryonic stem cells and tissue stem cells, have been considered as candidates for transplantation therapy. Bone marrow stromal cells (MSCs) have great potential as therapeutic agents since they are easy to isolate and can be expanded from patients without serious ethical or technical problems. We discovered a new method for the highly efficient and specific induction of functional Schwann cells, neurons and skeletal muscle lineage cells from both rat and human MSCs. These induced cells were transplanted into animal models of neurotraumatic injuries, Parkinson's disease, stroke and muscle dystrophies, resulting in the successful integration of transplanted cells and an improvement in behavior of the transplanted animals. Here we focus on the respective potentials of MSCderived cells and discuss the possibility of clinical application in degenerative diseases.

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Transplantation of neural stem cells into the spinal cord after injury

Cited by 143 publications

References 50 publications

Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells

Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

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