Transplantation of neural cells derived from retinoic acid-treated cynomolgus monkey embryonic stem cells successfully improved motor function of hemiplegic mice with experimental brain injury

Ikeda, Ritsuko; Kurokawa, Manae S.; Chiba, Shunmei; Yoshikawa, Hideshi; Ide, Michiko; Tadokoro, Mamoru; Nito, Shinji; Nakatsuji, Norio; Kawakami, Yasushi; Nagata, Ken; Hashimoto, Tsuyoshi; Suzuki, Noboru

doi:10.1016/j.nbd.2005.01.031

Cited by 73 publications

(48 citation statements)

References 50 publications

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“…Following the establishment of cultured human embryonic stem cell lines [26], several groups, including ours, have developed methods to generate NSCs from human or primate embryonic stem cells and have demonstrated their ability to enhance functional recovery following IC administration in preclinical models of stroke [21,[33][34][35][36][37]. Interestingly, in a study comparing IC delivery of embryonicand adult-derived NSCs into stroke-injured rats, embryonic-derived NSCs exhibited substantially higher survival that was correlated with a decreased host immune response [38].…”

Section: Embryonic-derived Neural Stem Cellsmentioning

confidence: 99%

Neural Stem Cells in Stroke: Intracerebral Approaches

Manley

Azevedo-Pereira

Bliss

et al. 2015

Cell Therapy for Brain Injury

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Section: Embryonic-derived Neural Stem Cellsmentioning

confidence: 99%

Neural Stem Cells in Stroke: Intracerebral Approaches

Manley

Azevedo-Pereira

Bliss

et al. 2015

Cell Therapy for Brain Injury

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“…Immunofluorescence staining was conducted as reported previously [16][17][18] . In brief, for all immunofluorescence procedures, cultured cells were fixed in 4 % paraformaldehyde for 15 minutes.…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells

Shiratsuchi

Misawa

Saito

et al. 2015

J St. Marianna Univ

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We have reported that mouse embryonic stem (ES) cells transfected with insulin-like growth factor (IGF) II differentiated into mature skeletal muscle cells in vitro and in vivo after transplantation. On the contrary, IGFII transfected human induced pluripotent stem (hiPS) cells did not demonstrate mature skeletal muscle differentiation. The morphogenic factor Sonic Hedgehog (SHH) was suggested to upregulate the myogenic process along with IGF through Phosphoinositide 3 kinase (PI3K)/Akt signaling pathway. We cultured hiPS cells with SHH to generate myoblasts and transplanted the cells to hind limbs of mice.SHH with IGFII supplementation rapidly enhanced myogenic gene and protein expressions (MyoD, myogenin, Mrf4, and dystrophin) of hiPS cells. After the transplantation, we observed severe inflammation in the transplanted sites with host immunocompetent cells despite systemic administration of dexamethasone and cyclosporine. Surviving transplanted myoblasts showed lower expressions of myogenic proteins (MyoD, myogenin, and dystrophin) than in vitro cultured myoblasts did.We successfully generated mature skeletal muscle cells from hiPS cells with SHH supplementation. We suggest that further studies are needed to characterize the underlying molecular mechanisms of transplanted myoblasts derived from hiPS cells for the formation of mature human skeletal muscle.

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“…Çoğu vakada greftler hücre yaşamı ve fonksiyonu için gerekli olan faktörleri aktive ederek etkili olmaktadır. Ancak klinik olarak etkinliğin olabilmesi için kök hücrelerin ölü nöronların yerine geçmesi, akson remyelinizasyonu yapmaları ve hasarlı nöronal devreleri onarmaları gerekir 6 . İlginç olan, hayvan modellerinde, hayvanın kendi nöral kök hücrelerinin zaman içinde hasarlı nöronların bir kısmının yerini alabildiği, strokdan aylar sonra oluşan yeni striatal nöronların hasarlanmanın olduğu bölgeye doğru göç ettiği gösterilmiştir 3,7 .…”

Section: Strok (İnme)unclassified

Nörolojik Hastalıklarda Kök Hücre Nakli

Hergüner¹

2014

Arşiv Kaynak Tarama Dergisi

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Neurological diseases are characterized by loss of cells in response to an injury or a progressive insult. In recent years, neurons and glia have been generated successfully from stem cells in cultures, fuelling efforts to develop stem-cell-based transplantation therapies for human neurological diseases, such as Parkinson disease, stroke or neuromuscular disorders. In this review, the potential of various cell therapies in childhood neurological diseases are discussed. Key words: Stem-cell therapy, neurological disorders, childhood. ÖZETNörolojik hastalıklar, sıklıkla nöronlarda kayıp veya progresif hasar nedeniyle oluşur. Son yıllarda kök hücre teknikleri ile gerek nöron, gerekse gliaların üretilmesi mümkün olmuş, bu gelişmelere paralel olarak da Parkinson, stroke, kas hastalıkları gibi pek çok nörolojik hastalığın tedavisinde kullanım çalışmaları başlamıştır. Bu yazıda, çocukluk döneminde çeşitli nörolojik hastalıkların tedavisinde hücre tedavilerinin rolü tartışılmıştır. Anahtar kelimeler: Kök hücre tedavisi, nörolojik hastalıklar, çocukluk dönemi. GirişKök hücre tedavisinin, beyin ve spinal kordu etkileyen pek çok hastalığın tedavisinde, gerek etkilenen nöron ve gliaların yenilenmesinde, gerekse nöroproduksiyonda önemli rol oynayacağı düşünülmektedir. Bu uygulama için embriyonik kök hücreler, fetal veya erişkin sinir sistemi kaynaklı hücreler veya diğer organlardan elde edilebilen kök hücreler kullanılabilir 1 . Ancak bu hücrelerin insan kaynaklı olmaları önerilmektedir. Kök hücre

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Transplantation of neural cells derived from retinoic acid-treated cynomolgus monkey embryonic stem cells successfully improved motor function of hemiplegic mice with experimental brain injury

Cited by 73 publications

References 50 publications

Neural Stem Cells in Stroke: Intracerebral Approaches

Neural Stem Cells in Stroke: Intracerebral Approaches

Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells

Nörolojik Hastalıklarda Kök Hücre Nakli

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