Nörolojik Hastalıklarda Kök Hücre Nakli

Hergüner, M. Özlem

doi:10.17827/aktd.09093

Cited by 1 publication

(2 citation statements)

References 38 publications

(47 reference statements)

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“…They are classified as embryonic and adult according to their origin. Since the first embryonic stem cell growth in culture medium in 1998, experimental studies and investigations related to stem cell treatment have been conducted on many neurological degenerative disorders mainly spinal cord injuries, cerebral infarct, Parkinson disease, AD (83)(84)(85)(86)(87)(88)(89)(90)(91). Mainly two stem cell types are used: pluripotent stem cell/induced pluripotent stem cell originated from embryonic stem cell, and adult stem cells (neuronal stem cells, hematopoietic stem cells, mesenchymal stem cells).…”

Section: Discussionmentioning

confidence: 99%

“…Mainly two stem cell types are used: pluripotent stem cell/induced pluripotent stem cell originated from embryonic stem cell, and adult stem cells (neuronal stem cells, hematopoietic stem cells, mesenchymal stem cells). Neural stem cells are primarily located in hippocampal dental gyrus and subventricular zone in CNS (83)(84)(85)89). These cells are activated during conditions such as cerebral infarct, and facilitate recovery.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of Spatial Learning and Memory Impairments by Fetal Neural Tissue Transplantation in Experimental Rat Model of Alzheimer’s Disease

Can¹,

Barlas²

2019

BMB

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It is known that the acetylcholinergic afferents of the neocortex from subcortical areas participate in learning and memory. Autopsy studies in cases of Alzheimer's disease (AD) have shown that most of the neurons of nucleus basalis magnocellularis (NBM) are atrophic or decreased in number. In this study, we searched for whether or not it was possible to improve the impaired learning and memory functions with foetal neural tissue transplantation in an experimental model of AD. Method: A total of thirty seven young adult male Wistar albino rats were served as experimental subjects. NBM on the right side was destroyed by the injection of kainic acid stereotactically so as to make a model of AD. The grafts were obtained from 14-16 day foetuses of the same genus. After the tissue with cholinergic neurons dissected from ventral forebrain and tissue with non-cholinergic neurons dissected from telencephalic vesicle, cell suspensions were prepared and injected stereotactically to the ipsilateral frontal cortex. Spatial learning and memory functions were tested by Morris' water maze tasks. Results: Spatial learning and memory functions in rats were impaired by unilateral lesions of nucleus basalis magnocellularis. The impairment observed during the early period partially improved by the time. It was observed that this amelioration was accelerated with both cholinergic and non-cholinergic foetal neural tissue implantation. Amaç: Subkortikal alanlardan neokortekse uzanan asetilkolinerjik nöronların öğrenme ve bellek süreçlerinde rol aldığı bilinmektedir. Alzheimer hastalığında (AH) yapılan otopsi incelemeleri bazal magnoselüler çekirdekteki nöronlarda atrofi veya azalma olduğunu göstermiştir. Bu çalışmada deneysel AH modelinde bozuk öğrenme ve bellek fonksiyonlarının, fötal nöral doku transplantasyonu ile düzelmesinin mümkün olup olmadığı araştırıldı. Yöntem: Deneysel çalışmada 37 adet Wistar albino cinsi genç erişkin erkek sıçan kullanıldı. AH modeli sağ bazal magnoselüler çekirdeğe stereotaktik yöntemle verilen, nörotoksik bir ajan olan kainik asit ile oluşturuldu. Greftler aynı cins sıçanların 14-16 günlük fetuslarından alındı. Kolinerjik nöronları içeren doku ventral ön beyinden, non-kolinerjik nöronların olduğu doku ise telensefalik vezikülden elde edilerek, hücre süspansiyonu haline getirildi ve stereotaktik yöntemle ipsilateral frontal kortekse implante edildi. Hacimsel öğrenme ve bellek fonksiyonları Morris'in "water maze" testi ile değerlendirildi. Bulgular: Tek taraflı bazal magnoselüler nükleus lezyonları ile sıçanlarda hacimsel öğrenme ve bellek fonksiyonları bozuldu. Erken dönemde bozulan bu fonksiyonlar geç dönemde kısmen düzeldi. Hem kolinerjik hem de non-kolinerjik fötal doku implantasyonu ile bu düzelmenin hızlandığı saptandı.

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