Transplantation of isolated islets of langerhans in diabetic dogs. I. Results after allogeneic intraportal islet transplantation

Lorenz, D.; Lippert, H.; Tietz, W; Worm, V.; Hahn, Hans-Jürgen; Dorn, A.; G, Koch; Ziegler, M.; Rosenbaum, K D

doi:10.1016/0022-4804(79)90129-x

Cited by 12 publications

(7 citation statements)

References 41 publications

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“…Islets were first transplanted into the liver via the portal vein in 1973 by the Lacy group and subsequent studies have shown that fewer islets are needed to ameliorate diabetes when transplanted into the portal venous drainage than other sites. [34][35][36][37][38] Transplantation of collagenase-isolated islets from large animal pancreata such as pig, 39 monkey 40 and dog 41 met with short-term success, but limited analysis of post-transplant graft function was presented. 42 Initial clinical trials of both auto-and allotransplantation followed quickly from the Sutherland group and others: these showed promise, [43][44][45][46] but again with limited evaluation of graft function and without long-term insulin independence.…”

Section: The Path From Islet Isolation To Transplantationmentioning

confidence: 99%

“…Transplantation of collagenase‐isolated islets from large animal pancreata such as pig, 39 monkey 40 and dog 41 met with short‐term success, but limited analysis of post‐transplant graft function was presented 42 . Initial clinical trials of both auto‐ and allotransplantation followed quickly from the Sutherland group and others: these showed promise, 43‐46 but again with limited evaluation of graft function and without long‐term insulin independence.…”

Section: The Path From Islet Isolation To Transplantationmentioning

confidence: 99%

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From insulin injections to islet transplantation: An overview of the journey

Paget

Murray

Bailey

et al. 2021

Diabetes Obesity Metabolism

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When, in 1869, Paul Langerhans detected the "islands of tissue" in the pancreas, he took the first step on a journey towards islet transplantation as a treatment for type 1 diabetes. The route has embraced developments across biosciences, surgery, gene therapy and clinical research. This review highlights major milestones along that journey involving whole pancreas transplantation, islet transplantation, the creation of surrogate insulinsecreting cells and novel islet-like structures using genetic and bio-engineering technologies. To obviate the paucity of human tissue, pluripotent stem cells and non-β-cells within the pancreas have been modified to create physiologically responsive insulinsecreting cells. Before implantation, these can be co-cultured with endothelial cells to promote vascularisation and with immune defence cells such as placental amnion cells to reduce immune rejection. Scaffolds to contain grafts and facilitate surgical placement provide further opportunities to achieve physiological insulin delivery. Alternatively, xenotransplants such as porcine islets might be reconsidered as opportunities exist to circumvent safety concerns and immune rejection. Thus, despite a long and arduous journey, the prospects for increased use of tissue transplantation to provide physiological insulin replacement are drawing ever closer.

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Section: The Path From Islet Isolation To Transplantationmentioning

confidence: 99%

Section: The Path From Islet Isolation To Transplantationmentioning

confidence: 99%

From insulin injections to islet transplantation: An overview of the journey

Paget

Murray

Bailey

et al. 2021

Diabetes Obesity Metabolism

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“…Naturally enough, the first method to be applied to the more fibrous large mammalian pancreas was that developed for the rat. Initial reports on monkey pancreas were encouraging [5] and the technique was applied to transplantation studies in the pig [70], monkey [71], and dog [72]. The islet preparation was not satisfactory in the pig experiments [70] and, although claimed to be satisfactory in the monkey [71] and dog [72], inadequate information was presented to confirm these claims and the findings have not been confirmed by other workers.…”

Section: Isolation Of Islets From the Pancreas Of Large Nonhuman Mammalsmentioning

confidence: 99%

Prospects for pancreatic islet transplantation

Gray

Morris

1986

World j. surg.

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It is over a decade since the first description of cure of experimental diabetes in rats by transplantation of isolated adult islets of Langerhans. Despite a remarkable number of experimental studies, clinical islet transplantation is still not a reality, raising questions about the feasibility of this approach. Experiments in rodents have demonstrated the potential of isolated islet transplantation. It is possible to cure experimentally induced diabetes and restore normal glucose metabolism with prevention or even reversal of diabetic complications. The problem of allograft rejection, while formidable, is not insurmountable and a number of techniques are now available to achieve this aim.Studies in animals larger than the rodent have been less successful and no repeatable method for isolated islet transplantation has so far emerged. Attempts at clinical purified islet transplantation have also proven unsuccessful. Transplantation of unpurified dispersed pancreatic tissue has been used successfully in dogs, but the technique has proven dangerous and inefficient when applied clinically. These disappointing results suggest, superficially, that the prospects for clinical islet transplantation are bleak. In reality, the problem in all the studies conducted in large mammals and humans has been the lack of an efficient method for separation of viable purified islets from the human and large mammalian pancreas. The recent description of more efficient islet separation methods allows new hope for clinical transplantation.

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“…In large animals, diabetes has been induced either by total pancreatectomy [83,84], an absolutely reliable model for both autograft and allograft experiments, but complicated by exocrine deficiency; or by streptozotocin and alloxan with or without partial pancreatectomy [85,86], a less reliable model of diabetes and one that makes autotransplant experiments difficult. This is an important drawback because the lack of inbred strains dictates the use of allogeneic donors in situations where the immunological and metabolic consequences of the transplant are not easily distinguished.…”

Section: Ill Experimental Transplantation Of Free Grafts Of Islet Tismentioning

confidence: 99%

“…The collagenase digestion/Ficoll gradient separation technique has been used to isolate viable islets from mouse [97], dog [86], pig [98], monkey [85], and human pancreases [99]. In general, application to large animals has been difficult [100].…”

Section: Isolation Of Islets From Adultmentioning

confidence: 99%

Pancreas and islet transplantation

Sutherland

1981

Diabetologia

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Transplantation of isolated islets of langerhans in diabetic dogs. I. Results after allogeneic intraportal islet transplantation

Cited by 12 publications

References 41 publications

From insulin injections to islet transplantation: An overview of the journey

From insulin injections to islet transplantation: An overview of the journey

Prospects for pancreatic islet transplantation

Pancreas and islet transplantation

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