Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improves Cartilage Repair in a Rabbit Model

Yang, Guihua; Shao, Jiashen; Lin, Jiachen; Yang, Haixia; Jin, Jing; Yu, Chenxi; Shen, Bo; Hu, Xiaorui; Si, Huijie; Li, Xiaoxin; Niu, Yuchen; Wu, Zhihong

doi:10.1155/2021/6380141

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…After 8 weeks after injection, the defect in the MSCs-UB injection group was reduced by 1/2, while in the MSCs-BM group it was reduced by only 1/3 [46].…”

Section: Results Discussionmentioning

confidence: 86%

“…However, it is worth noting that the simulation of joint cartilage damage in numerous publications is carried out surgically, creating mechanical damage to the cartilage [46]. Therefore, some researchers, considering the prospects of using MSCs in acute cartilage injury, take into account the data obtained in such studies [43].…”

Section: Results Discussionmentioning

confidence: 99%

“…The central reason for this is the biological properties of cartilage tissue. For example, articular cartilage is known to have a limited and insufficient capacity for self-healing, and to date no standard treatment considered effective can fully restore it after damage [46]. Therefore, cell therapy strategies involving stem cells from a wide range of tissues are attracting increasing attention both in research and when used in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

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Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Fishchenko¹,

Botsul²,

Shuvalova³

et al. 2022

Rep. of Vinnytsia Nation. Med. Univ.

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Annotation. Since standard methods of treatment of cartilage trauma and inflammatory diseases lack effectiveness, at present one of the most promising strategies is mesenchymal stem cell transplantation. Despite the successes in this field, many issues, such as the selection of the tissue-source of mesenchymal stem cells (MSCs) and their optimization or modification in culture in order to achieve the maximum therapeutic effect, remains an actual task for both global and domestic scientists. The purpose of the study is to prepare a review of literature sources for the period 2004-2022 (in the vast majority on publications of the last five years) and to systematize new data on the biological characteristics of MSCs from various tissue sources, which are used in strategies for the treatment of cartilage lesions tissues, to analyze existing problems and further prospects, as well as potential ways of optimizing MSC cultivation methods to increase their regenerative potential. A study of 49 sources that met the inclusion criteria was carried out. A significant variety of strategies for the use of MSCs in animal models and in clinical practice was revealed. The main mechanisms ensuring the therapeutic effect of MSCs have been determined and can be used as criteria for optimizing cellular material. However, it was found that the question of the effectiveness of the use of differentiated MSCs, the selection of the tissue-source of MSCs, and the specifics of the use of MSCs from neonatal sources remains open. Thus, the analysis of literary sources revealed further promising ways to improve the therapeutic efficiency of MSC grafting in a wide range of cartilaginous lesions. There is a need to research the effects of MSCs from neonatal tissues, and especially the umbilical cord. This will become possible due to experimental research that will be carried out in the future.

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“…After 8 weeks after injection, the defect in the MSCs-UB injection group was reduced by 1/2, while in the MSCs-BM group it was reduced by only 1/3 [46].…”

Section: Results Discussionmentioning

confidence: 86%

Section: Results Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Fishchenko¹,

Botsul²,

Shuvalova³

et al. 2022

Rep. of Vinnytsia Nation. Med. Univ.

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“…The surface of cartilage slices was evenly covered with DAB, stained with hematoxylin, and soaked in 1% hydrochloric acid alcohol after rinsing with distilled water for 5 min. Tissue samples were deparaffinized and rehydrated by using xylene substitute and graded alcohols [ 34 ]. The images were observed using a microscope (Olympus BX-50, Tokyo, Japan) with high magnification (×200).…”

Section: Methodsmentioning

confidence: 99%

Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis

Liu

Wei

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The current study was designed to investigate the effect and underlying mechanism of warm acupuncture combined with bone marrow mesenchymal stem cells (BMSC) transplantation on cartilage tissue injury in rabbit knee osteoarthritis (KOA). In the study, 50 rabbits were randomly divided into 5 groups: blank group, KOA group, warm acupuncture group, BMSCs group, and warm acupuncture combined with BMSCs group. After warm acupuncture combined with BMSCs, the Modified Lequesne MG knee joint assessment scale was used to evaluate the degree of knee joint behavior, the Taiping Peng method generally observed the histomorphology changes of KOA rabbit cartilage, and hematoxylin-eosin staining, safranin O green staining, and toluidine blue staining were conducted to evaluate the extent of cartilage tissue pathology. Furthermore, transmission electron microscopy and TUNEL staining were used to observe cell apoptosis, and immunohistochemistry and qPCR analysis were used to detect the expression of apoptosis-related proteins and mRNA. Results showed that administration of warm acupuncture combined with BMSCs recovered the joint function and significantly decreased Lequesne MG score. The degree of cartilage tissue pathological damage has been improved, cartilage ultrastructure degeneration has recovered, peripheral blood vessels have mild edema, blood supply has gradually recovered, and even small amounts of red blood cells have appeared. In addition, warm acupuncture combined with BMSCs treatment suppressed chondrocyte apoptosis in rabbits with knee osteoarthritis by reduced TUNEL-positive chondrocytes and simultaneously reversed the mRNA expression of Bax, Bcl-2, and Caspase-3. These results indicate that warm acupuncture combined with BMSCs transplantation has a potential protective effect on rabbit KOA, which may be mediated by inhibiting chondrocyte apoptosis.

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“…In addition, Yan et al verified that the umbilical cord MSCs (UMSCs-EXO) exosomes had powerful cartilage tissue protection effects, containing activating the proliferation and migration of chondrocytes, enhancing matrix synthesis and impeding chondrocyte apoptosis [39]. In the constructed animal model of cartilage defect in vivo, after intra-articular injection of UMSCs-EXO, the cartilage defect was repaired well [40].…”

Section: Umscs Derived Exosomesmentioning

confidence: 99%

Biomaterials-assisted exosomes therapy in osteoarthritis

Chen

Wang

et al. 2022

Biomed. Mater.

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Due to the avascular characteristic of articular cartilage, its self-repair capacity is limited. When cartilage is damaged or forms osteoarthritis, clinical treatment is necessary. However, conventional treatments, including joint replacement, microfracture, cell and drug therapies, have certain limits. Lately, the exosomes derived from mesenchymal stem cells (MSCs-EXO), which consist of complex transcription factors, proteins and targeting ligand components, have shown great therapeutic potentials. With recent advancements in various biomaterials to extend MSCs-EXO’s retention time and control the release properties in vivo, biomaterials-assisted exosomes therapy has been soon becoming a practically powerful tool in treating OA. This review analyzes the effects of MSCs-EXO on osteoarthritis inflammation, metabolism, ageing and apoptosis, and introduces the combinational systems of MSCs-EXO with biomaterials to enhance the repair, anti-inflammatory, and homeostasis regulation functions. Moreover, different types of natural or synthetic biomaterials and their applications with MSCs-EXO were also described and discussed. And finally, we presage the future perspective in the development of biomaterial-assisted exosome therapies, as well as the potential to incorporate with other treatments to enhance their therapeutic effects in osteoarthritis.

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Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improves Cartilage Repair in a Rabbit Model

Cited by 7 publications

References 27 publications

Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis

Biomaterials-assisted exosomes therapy in osteoarthritis

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